Endothelial progenitor cells (EPCs) as gene carrier system for rat model of human glioma

Nadimpalli Ravi S. Varma, Branislava Janic, A. S.M. Iskander, Adarsh Shankar, Mohammed P.I. Bhuiyan, Hamid Soltanian-Zadeh, Quan Jiang, Kenneth Barton, Meser M. Ali, Ali Syed Arbab

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Due to their unique property to migrate to pathological lesions, stem cells are used as a delivery vehicle for therapeutic genes to tumors, especially for glioma. It is critically important to track the movement, localization, engraftment efficiency and functional capability or expression of transgenes of selected cell populations following transplantation. The purposes of this study were to investigate whether 1) intravenously administered, genetically transformed cord blood derived EPCs can carry human sodium iodide symporter (hNIS) to the sites of tumors in rat orthotopic model of human glioma and express transgene products, and 2) whether accumulation of these administered EPCs can be tracked by different in vivo imaging modalities. Methods and Results: Collected EPCs were cultured and transduced to carry hNIS. Cellular viability, differential capacity and Tc-99m uptake were determined. Five to ten million EPCs were intravenously administered and Tc-99-SPECT images were acquired on day 8, to determine the accumulation of EPCs and expression of transgenes (increase activity of Tc-99m) in the tumors. Immunohistochemistry was performed to determine endothelial cell markers and hNIS positive cells in the tumors. Transduced EPCs were also magnetically labeled and accumulation of cells was confirmed by MRI and histochemistry. SPECT analysis showed increased activity of Tc-99m in the tumors that received transduced EPCs, indicative of the expression of transgene (hNIS). Activity of Tc-99m in the tumors was also dependent on the number of administered transduced EPCs. MRI showed the accumulation of magnetically labeled EPCs. Immunohistochemical analysis showed iron and hNIS positive and, human CD31 and vWF positive cells in the tumors. Conclusion: EPC was able to carry and express hNIS in glioma following IV administration. SPECT detected migration of EPCs and expression of the hNIS gene. EPCs can be used as gene carrier/delivery system for glioma therapy as well as imaging probes.

Original languageEnglish (US)
Article numbere30310
JournalPloS one
Volume7
Issue number1
DOIs
StatePublished - Jan 20 2012
Externally publishedYes

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Endothelial cells
Glioma
endothelial cells
stem cells
Rats
Genes
animal models
symporters
iodides
Tumors
genes
sodium
Transgenes
neoplasms
transgenes
Single-Photon Emission-Computed Tomography
Neoplasms
Cells
Endothelial Progenitor Cells
Magnetic resonance imaging

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Varma, N. R. S., Janic, B., Iskander, A. S. M., Shankar, A., Bhuiyan, M. P. I., Soltanian-Zadeh, H., ... Arbab, A. S. (2012). Endothelial progenitor cells (EPCs) as gene carrier system for rat model of human glioma. PloS one, 7(1), [e30310]. https://doi.org/10.1371/journal.pone.0030310

Endothelial progenitor cells (EPCs) as gene carrier system for rat model of human glioma. / Varma, Nadimpalli Ravi S.; Janic, Branislava; Iskander, A. S.M.; Shankar, Adarsh; Bhuiyan, Mohammed P.I.; Soltanian-Zadeh, Hamid; Jiang, Quan; Barton, Kenneth; Ali, Meser M.; Arbab, Ali Syed.

In: PloS one, Vol. 7, No. 1, e30310, 20.01.2012.

Research output: Contribution to journalArticle

Varma, NRS, Janic, B, Iskander, ASM, Shankar, A, Bhuiyan, MPI, Soltanian-Zadeh, H, Jiang, Q, Barton, K, Ali, MM & Arbab, AS 2012, 'Endothelial progenitor cells (EPCs) as gene carrier system for rat model of human glioma', PloS one, vol. 7, no. 1, e30310. https://doi.org/10.1371/journal.pone.0030310
Varma NRS, Janic B, Iskander ASM, Shankar A, Bhuiyan MPI, Soltanian-Zadeh H et al. Endothelial progenitor cells (EPCs) as gene carrier system for rat model of human glioma. PloS one. 2012 Jan 20;7(1). e30310. https://doi.org/10.1371/journal.pone.0030310
Varma, Nadimpalli Ravi S. ; Janic, Branislava ; Iskander, A. S.M. ; Shankar, Adarsh ; Bhuiyan, Mohammed P.I. ; Soltanian-Zadeh, Hamid ; Jiang, Quan ; Barton, Kenneth ; Ali, Meser M. ; Arbab, Ali Syed. / Endothelial progenitor cells (EPCs) as gene carrier system for rat model of human glioma. In: PloS one. 2012 ; Vol. 7, No. 1.
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AU - Shankar, Adarsh

AU - Bhuiyan, Mohammed P.I.

AU - Soltanian-Zadeh, Hamid

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