Endothelin-1/Endothelin A receptor-mediated biased signaling is a new player in modulating human ovarian cancer cell tumorigenesis

Jian peng Teoh, Kyoung mi Park, Yongchao Wang, Qiuping Hu, Sangmi Kim, Guangyu Wu, Shuang Huang, Nita Jane Maihle, Il-man Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The endothelin-1 (ET-1)/endothelin A receptor (ET A R, a G protein-coupled receptor) axis confers pleiotropic effects on both tumor cells and the tumor microenvironment, modulating chemo-resistance and other tumor-associated processes by activating Gαq- and β-arrestin-mediated pathways. While the precise mechanisms by which these effects occur remain to be elucidated, interference with ET A R signaling has emerged as a promising antitumor strategy in many cancers including ovarian cancer (OC). However, current clinical approaches using ET A R antagonists in the absence of a detailed knowledge of downstream signaling have resulted in multiple adverse side effects and limited therapeutic efficacy. To maximize the safety and efficacy of ET A R-targeted OC therapy, we investigated the role of other G protein subunits such as Gα s in the ET A R-mediated ovarian oncogenic signaling. In HEY (human metastatic OC) cells where the ET-1/ET A R axis is well-characterized, Gαs signaling inhibits ET A R-mediated OC cell migration, wound healing, proliferation and colony formation on soft agar while inducing OC cell apoptosis. Mechanistically, ET-1/ET A R is coupled to Gαs/cAMP signaling in the same ovarian carcinoma-derived cell line. Gαs/cAMP/PKA activation inhibits ET A R-mediated β-arrestin activation of angiogenic/metastatic Calcrl and Icam2 expression. Consistent with our findings, Gαs overexpression is associated with improved survival in OC patients in the analysis of the Cancer Genome Atlas data. In conclusion, our results indicate a novel function for Gαs signaling in ET-1/ET A R-mediated OC oncogenesis and may provide a rationale for a biased signaling mechanism, which selectively activates Gαs-coupled tumor suppressive pathways while blocking Gαq-/β-arrestin-mediated oncogenic pathways, to improve the targeting of the ET A R axis in OC.

Original languageEnglish (US)
Pages (from-to)2885-2895
Number of pages11
JournalCellular Signalling
Volume26
Issue number12
DOIs
StatePublished - Sep 3 2014

Fingerprint

Endothelin A Receptors
Endothelin-1
Ovarian Neoplasms
Carcinogenesis
Arrestin
Neoplasms
Cellular Microenvironment
Tumor Microenvironment
Atlases
Protein Subunits
Therapeutic Uses
G-Protein-Coupled Receptors
GTP-Binding Proteins
Wound Healing
Agar
Cell Movement
Genome
Apoptosis
Carcinoma
Safety

Keywords

  • Biased G protein-coupled receptor signaling
  • Endothelin axis
  • Metastatic genes
  • β-arrestin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Endothelin-1/Endothelin A receptor-mediated biased signaling is a new player in modulating human ovarian cancer cell tumorigenesis. / Teoh, Jian peng; Park, Kyoung mi; Wang, Yongchao; Hu, Qiuping; Kim, Sangmi; Wu, Guangyu; Huang, Shuang; Maihle, Nita Jane; Kim, Il-man.

In: Cellular Signalling, Vol. 26, No. 12, 03.09.2014, p. 2885-2895.

Research output: Contribution to journalArticle

Teoh, Jian peng ; Park, Kyoung mi ; Wang, Yongchao ; Hu, Qiuping ; Kim, Sangmi ; Wu, Guangyu ; Huang, Shuang ; Maihle, Nita Jane ; Kim, Il-man. / Endothelin-1/Endothelin A receptor-mediated biased signaling is a new player in modulating human ovarian cancer cell tumorigenesis. In: Cellular Signalling. 2014 ; Vol. 26, No. 12. pp. 2885-2895.
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abstract = "The endothelin-1 (ET-1)/endothelin A receptor (ET A R, a G protein-coupled receptor) axis confers pleiotropic effects on both tumor cells and the tumor microenvironment, modulating chemo-resistance and other tumor-associated processes by activating Gαq- and β-arrestin-mediated pathways. While the precise mechanisms by which these effects occur remain to be elucidated, interference with ET A R signaling has emerged as a promising antitumor strategy in many cancers including ovarian cancer (OC). However, current clinical approaches using ET A R antagonists in the absence of a detailed knowledge of downstream signaling have resulted in multiple adverse side effects and limited therapeutic efficacy. To maximize the safety and efficacy of ET A R-targeted OC therapy, we investigated the role of other G protein subunits such as Gα s in the ET A R-mediated ovarian oncogenic signaling. In HEY (human metastatic OC) cells where the ET-1/ET A R axis is well-characterized, Gαs signaling inhibits ET A R-mediated OC cell migration, wound healing, proliferation and colony formation on soft agar while inducing OC cell apoptosis. Mechanistically, ET-1/ET A R is coupled to Gαs/cAMP signaling in the same ovarian carcinoma-derived cell line. Gαs/cAMP/PKA activation inhibits ET A R-mediated β-arrestin activation of angiogenic/metastatic Calcrl and Icam2 expression. Consistent with our findings, Gαs overexpression is associated with improved survival in OC patients in the analysis of the Cancer Genome Atlas data. In conclusion, our results indicate a novel function for Gαs signaling in ET-1/ET A R-mediated OC oncogenesis and may provide a rationale for a biased signaling mechanism, which selectively activates Gαs-coupled tumor suppressive pathways while blocking Gαq-/β-arrestin-mediated oncogenic pathways, to improve the targeting of the ET A R axis in OC.",
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AU - Hu, Qiuping

AU - Kim, Sangmi

AU - Wu, Guangyu

AU - Huang, Shuang

AU - Maihle, Nita Jane

AU - Kim, Il-man

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