Epidermal growth factor, a vascular smooth muscle mitogen, induces rat aortic contraction

B. C. Berk, T. A. Brock, R Clinton Webb, M. B. Taubman, W. J. Atkinson, M. A. Gimbrone, R. W. Alexander

Research output: Contribution to journalArticle

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Abstract

Atherosclerotic arteries have enhanced reactivity to vasoconstrictors, which suggests that features of the atherosclerotic process itself may result in this abnormal responsiveness. Since vascular smooth muscle proliferation is a prominent feature of atherosclerosis, we postulated that vasoactive agonists and smooth muscle mitogens may share certain common cellular mechanisms of action which potentially contribute to this hyperreactivity. To test the hypothesis, we studied the effects of epidermal growth factor (EGF), a well-characterized mitogen, on rat aortic vascular smooth muscle, both in intact aortic strips and in culture. EGF caused contraction (EC50 = 19 nM) of rat aortic strips which maximally was equivalent to 40% of that induced by angiotensin II, a potent vasoconstrictor. EGF increased 45Ca efflux (EC50 = 3 nM) from cultured rat aortic smooth muscle cells, which was an effect shared by angiotensin II and thought to reflect increased cytosolic-free calcium concentration. EGF (7.5 nM) also stimulated growth of these cultured cells to the same extent as 10% calf serum. These results demonstrate that EGF is both a vasoconstrictor and mitogen for rat aortic smooth muscle cells. The similarities in the effects of EGF and angiotensin II suggest that certain common intracellular mechanisms of action may exist for vasoactive agonists and growth factors which may contribute to the altered vasoreactivity of atherosclerotic vessels.

Original languageEnglish (US)
Pages (from-to)1083-1086
Number of pages4
JournalJournal of Clinical Investigation
Volume75
Issue number3
DOIs
StatePublished - Jan 1 1985

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Vascular Smooth Muscle
Mitogens
Epidermal Growth Factor
Vasoconstrictor Agents
Angiotensin II
Smooth Muscle Myocytes
Smooth Muscle
Cultured Cells
Atherosclerosis
Intercellular Signaling Peptides and Proteins
Arteries
Calcium
Growth
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Berk, B. C., Brock, T. A., Webb, R. C., Taubman, M. B., Atkinson, W. J., Gimbrone, M. A., & Alexander, R. W. (1985). Epidermal growth factor, a vascular smooth muscle mitogen, induces rat aortic contraction. Journal of Clinical Investigation, 75(3), 1083-1086. https://doi.org/10.1172/JCI111772

Epidermal growth factor, a vascular smooth muscle mitogen, induces rat aortic contraction. / Berk, B. C.; Brock, T. A.; Webb, R Clinton; Taubman, M. B.; Atkinson, W. J.; Gimbrone, M. A.; Alexander, R. W.

In: Journal of Clinical Investigation, Vol. 75, No. 3, 01.01.1985, p. 1083-1086.

Research output: Contribution to journalArticle

Berk, BC, Brock, TA, Webb, RC, Taubman, MB, Atkinson, WJ, Gimbrone, MA & Alexander, RW 1985, 'Epidermal growth factor, a vascular smooth muscle mitogen, induces rat aortic contraction', Journal of Clinical Investigation, vol. 75, no. 3, pp. 1083-1086. https://doi.org/10.1172/JCI111772
Berk, B. C. ; Brock, T. A. ; Webb, R Clinton ; Taubman, M. B. ; Atkinson, W. J. ; Gimbrone, M. A. ; Alexander, R. W. / Epidermal growth factor, a vascular smooth muscle mitogen, induces rat aortic contraction. In: Journal of Clinical Investigation. 1985 ; Vol. 75, No. 3. pp. 1083-1086.
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