Epidermal growth factor and transforming growth factor a characteristics of human oral carcinoma cell lines

S. S. Prime, S. M. Gamel, J. B. Matthews, A. Stone, M. J. Donnelly, William Andrew Yeudall, V. Patel, R. Sposto, A. Silverthorne, C. Scully

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study examined the expression of epidermal growth factor (EGF) cell-surface receptors, the response to exogenous ligand and the autocrine production of transforming growth factor alpha (TGF-alpha) in normal and carcinoma-derived human oral keratinocytes. One of eight malignant cell lines overexpressed EGF receptors, while the remainder expressed receptor numbers similar to normal cells. Exogenous EGF stimulated incorporation of tritiated thymidine in a dose-dependent manner. In keratinocytes expressing normal numbers of EGF receptors, the cellular response to exogenous EGF correlated positively with total EGF receptor number. SCC-derived keratinocytes produced more TGF-alpha than normal cells. There was no statistical correlation between the autocrine production of TGF-alpha, EGF cell-surface receptor expression and cellular response to exogenous EGF. While the growth-stimulatory effects of exogenous TGF-alpha were inhibited by the addition of a neutralising antibody, the presence of this antibody in conditioned medium failed to produce a similar decrease in growth. The results indicate that overexpression of EGF receptors is not an invariable characteristic of human oral squamous carcinoma-derived cell lines. Further, the contribution of TGF-alpha to the growth of normal and carcinoma-derived human oral keratinocytes in vitro may be less significant than previously documented.

Original languageEnglish (US)
Pages (from-to)8-15
Number of pages8
JournalBritish Journal of Cancer
Volume69
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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Transforming Growth Factor alpha
Transforming Growth Factors
Epidermal Growth Factor
Keratinocytes
Carcinoma
Cell Line
Cell Surface Receptors
Growth
Conditioned Culture Medium
Neutralizing Antibodies
Epidermal Growth Factor Receptor
Thymidine
Squamous Cell Carcinoma
Ligands
Antibodies
ErbB Receptors

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Epidermal growth factor and transforming growth factor a characteristics of human oral carcinoma cell lines. / Prime, S. S.; Gamel, S. M.; Matthews, J. B.; Stone, A.; Donnelly, M. J.; Yeudall, William Andrew; Patel, V.; Sposto, R.; Silverthorne, A.; Scully, C.

In: British Journal of Cancer, Vol. 69, No. 1, 01.01.1994, p. 8-15.

Research output: Contribution to journalArticle

Prime, SS, Gamel, SM, Matthews, JB, Stone, A, Donnelly, MJ, Yeudall, WA, Patel, V, Sposto, R, Silverthorne, A & Scully, C 1994, 'Epidermal growth factor and transforming growth factor a characteristics of human oral carcinoma cell lines', British Journal of Cancer, vol. 69, no. 1, pp. 8-15. https://doi.org/10.1038/bjc.1994.2
Prime, S. S. ; Gamel, S. M. ; Matthews, J. B. ; Stone, A. ; Donnelly, M. J. ; Yeudall, William Andrew ; Patel, V. ; Sposto, R. ; Silverthorne, A. ; Scully, C. / Epidermal growth factor and transforming growth factor a characteristics of human oral carcinoma cell lines. In: British Journal of Cancer. 1994 ; Vol. 69, No. 1. pp. 8-15.
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