Epigenetic regulation of apoptosis and cell cycle regulatory genes in human colon carcinoma cells

Amy V. Paschall, Kebin Liu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

5-Fluorouracil (5-FU) is the standard chemotherapy for certain high risk stage 2 and all stage 3 and 4 human colorectal cancer patients. However, patients often develop chemoresistance to 5-FU. We have identified verticillin A from Verticillium-infected wild mushrooms as a potent anti-cancer agent that effectively suppresses 5-FU-resistant human colon cancer cells. Interestingly, a sublethal dose of verticillin A also acts as a potent sensitizer that overcomes human colon carcinoma cell resistance to FasL- and TRAIL-induced apoptosis. To identify verticillin A-regulated genes, we performed a genome-wide gene expression analysis and identified 1287 genes whose expression levels were either up- or down-regulated 1.5 fold. Forty-six of these genes have known function in regulation of apoptosis, and ninety genes have function in cell cycle regulation. Our recent study has identified verticillin A as a selective histone methyltransferase inhibitor. These identified genes are thus potential molecular targets for epigenetic-based therapy to overcome human colon cancer 5-FU resistance. The entire dataset is deposited in the NIH GEO database; accession number GSE51262.

Original languageEnglish (US)
Pages (from-to)189-191
Number of pages3
JournalGenomics Data
Volume5
DOIs
StatePublished - Sep 1 2015

Fingerprint

cdc Genes
Regulator Genes
Epigenomics
Fluorouracil
Colon
Genes
Cells
Apoptosis
Carcinoma
Colonic Neoplasms
Gene expression
Verticillium
Gene Expression
Agaricales
Chemotherapy
Colorectal Neoplasms
Cell Cycle
Genome
Databases
Drug Therapy

Keywords

  • Apoptosis
  • Cell cycle arrest
  • Colon cancer
  • H3K9 methylation
  • Histone methyltransferase

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Genetics

Cite this

Epigenetic regulation of apoptosis and cell cycle regulatory genes in human colon carcinoma cells. / Paschall, Amy V.; Liu, Kebin.

In: Genomics Data, Vol. 5, 01.09.2015, p. 189-191.

Research output: Contribution to journalArticle

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