Epitope repertoire of human CD4+ T cells on tetanus toxin: Identification of immunodominant sequence segments

B. M. Diethelm-Okita, R. Raju, D. K. Okita, B. M. Conti-Fine

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Sequence regions of tetanus toxin-forming CD4+ cell epitopes in 8 HLA- disparate subjects were identified. Overlapping synthetic peptides corresponding to the complete tetanus toxin sequence were used to test, in a proliferation assay, unselected blood CD4+ cells or CD4+ cell lines propagated by stimulation with tetanus toxoid. The CD4+ cell lines recognized most peptides recognized by the blood CD4+ cells and they recognized additional peptides. Their responses were stronger than those of unselected blood CD4+ cells. Two peptides were recognized by all subjects: one largely overlapped a tetanus toxin sequence region previously identified as a 'universal' T cell epitope. Thirteen other peptides elicited a CD4+ cell response in 6 or 7 of the 8 subjects, and another 10 elicited responses in 5 subjects.

Original languageEnglish (US)
Pages (from-to)382-391
Number of pages10
JournalJournal of Infectious Diseases
Issue number2
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


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