Erbin interacts with c-Cbl and promotes tumourigenesis and tumour growth in colorectal cancer by preventing c-Cbl-mediated ubiquitination and down-regulation of EGFR

Su Yao, Ping Zheng, Hua Wu, Li Ming Song, Xiao Fang Ying, Cheng Xing, Ying Li, Zheng Quan Xiao, Xing Ni Zhou, Tong Shen, Lin Chen, Yu Hong Liu, Mao De Lai, Lin Mei, Tian Ming Gao, Jian Ming Li

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The epidermal growth factor receptor (EGFR) is implicated in many types of cancer, including colorectal cancer (CRC), and has become one of the most common candidates for targeted therapy. Here, we found that Erbin, a member of the leucine-rich repeat and PDZ domain (LAP) family, plays a key role in EGFR signalling. Erbin inhibited EGFR ubiquitination and stabilized the EGFR protein by interacting with c-Cbl. Moreover, the PDZ domain of Erbin was critical for the interaction between Erbin and c-Cbl and EGFR ubiquitination. Interestingly, Erbin expression was elevated in tumour samples from CRC patients, increased in advanced clinical stage disease and correlated with EGFR expression. In vivo studies using mouse xenograft models of CRC showed that Erbin promotes tumour growth, and that the effects of Erbin on tumour growth are mainly related to the regulatory effects of Erbin on EGFR. The azoxymethane (AOM)-induced colon carcinogenesis model in ErbinΔC/ΔC mice, with the PDZ domain of Erbin deleted, demonstrated that the PDZ domain of Erbin and its regulation of EGFR signalling are necessary for the tumourigenesis and tumour growth of CRC. We found that Erbin promotes tumourigenesis and tumour growth in CRC by stabilizing EGFR. Our study sheds light on developing Erbin, especially its PDZ domain, as a potential target for CRC treatment.

Original languageEnglish (US)
Pages (from-to)65-77
Number of pages13
JournalJournal of Pathology
Volume236
Issue number1
DOIs
StatePublished - May 1 2015

Keywords

  • EGFR
  • Erbin
  • colorectal cancer
  • tumour growth
  • tumourigenesis
  • ubiquitination

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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