Abstract
Purpose: To directly test the effectiveness of ethanol-wet bonding (EW) in improving monomer infiltration into demineralized dentin through quantitative measurement of bis-GMA and TEG-DMA molar concentrations within hybrid layers, and to comprehensively evaluate the effect of EW and chlorhexidine on durability of resin-dentin bonds compared to conventional water-wet bonding (WW). Materials and Methods: A three-step etch-and-rinse adhesive (70% bis-GMA/28.75%TEG-DMA) was applied to coronal dentin using a clinically relevant ethanol-wet bonding protocol (EW) or the conventional water-wet bonding (WW) technique. Bis-GMA and TEG-DMA molar concentrations at various positions across the resin/dentin interfaces formed by EW and WW were measured using micro-Raman spectroscopy. The experiment was repeated at the same positions after 7-month storage in phosphate buffer solution containing 0.1% sodium azide. The μTBS and hybrid layer morphology (TEM) of bonding groups with and without chlorhexidine application were compared immediately and after 1-year storage in terms of nanoleakage, collagen fibril diameter, collagen interfibrillar width, and hybrid layer thickness. Results: Specimens bonded with EW showed significantly higher monomer molar concentrations and μTBS throughout the hybrid layer immediately and after storage, providing direct evidence of superior infiltration of hydrophobic monomers in EW compared to WW. Microscopically, EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. The application of chlorhexidine further preserved collagen integrity and limited the degree of nanoleakage in EW after 1-year storage. Conclusion: EW enhances infiltration of hydrophobic monomers into demineralized dentin. The results suggest that a more durable resin-dentin bond may be achieved with combined usage of a clinically relevant EW and chlorhexidine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 441-450 |
| Number of pages | 10 |
| Journal | Journal of Adhesive Dentistry |
| Volume | 16 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 2014 |
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Keywords
- Adhesives
- Bonding
- Collagen(s)
- Dentin
- Matrix metalloproteinases (MMPs)
- Ultrastructure
ASJC Scopus subject areas
- Orthodontics
- Oral Surgery
- Periodontics
Cite this
Ethanol-wet bonding and chlorhexidine improve resin-dentin bond durability : Quantitative analysis using Raman spectroscopy. / Talungchit, Supitcha; Jessop, Julie L.P.; Cobb, Deborah S.; Qian, Fang; Geraldeli, Saulo; Pashley, David Henry; Armstrong, Steven R.
In: Journal of Adhesive Dentistry, Vol. 16, No. 5, 01.01.2014, p. 441-450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ethanol-wet bonding and chlorhexidine improve resin-dentin bond durability
T2 - Quantitative analysis using Raman spectroscopy
AU - Talungchit, Supitcha
AU - Jessop, Julie L.P.
AU - Cobb, Deborah S.
AU - Qian, Fang
AU - Geraldeli, Saulo
AU - Pashley, David Henry
AU - Armstrong, Steven R.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Purpose: To directly test the effectiveness of ethanol-wet bonding (EW) in improving monomer infiltration into demineralized dentin through quantitative measurement of bis-GMA and TEG-DMA molar concentrations within hybrid layers, and to comprehensively evaluate the effect of EW and chlorhexidine on durability of resin-dentin bonds compared to conventional water-wet bonding (WW). Materials and Methods: A three-step etch-and-rinse adhesive (70% bis-GMA/28.75%TEG-DMA) was applied to coronal dentin using a clinically relevant ethanol-wet bonding protocol (EW) or the conventional water-wet bonding (WW) technique. Bis-GMA and TEG-DMA molar concentrations at various positions across the resin/dentin interfaces formed by EW and WW were measured using micro-Raman spectroscopy. The experiment was repeated at the same positions after 7-month storage in phosphate buffer solution containing 0.1% sodium azide. The μTBS and hybrid layer morphology (TEM) of bonding groups with and without chlorhexidine application were compared immediately and after 1-year storage in terms of nanoleakage, collagen fibril diameter, collagen interfibrillar width, and hybrid layer thickness. Results: Specimens bonded with EW showed significantly higher monomer molar concentrations and μTBS throughout the hybrid layer immediately and after storage, providing direct evidence of superior infiltration of hydrophobic monomers in EW compared to WW. Microscopically, EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. The application of chlorhexidine further preserved collagen integrity and limited the degree of nanoleakage in EW after 1-year storage. Conclusion: EW enhances infiltration of hydrophobic monomers into demineralized dentin. The results suggest that a more durable resin-dentin bond may be achieved with combined usage of a clinically relevant EW and chlorhexidine.
AB - Purpose: To directly test the effectiveness of ethanol-wet bonding (EW) in improving monomer infiltration into demineralized dentin through quantitative measurement of bis-GMA and TEG-DMA molar concentrations within hybrid layers, and to comprehensively evaluate the effect of EW and chlorhexidine on durability of resin-dentin bonds compared to conventional water-wet bonding (WW). Materials and Methods: A three-step etch-and-rinse adhesive (70% bis-GMA/28.75%TEG-DMA) was applied to coronal dentin using a clinically relevant ethanol-wet bonding protocol (EW) or the conventional water-wet bonding (WW) technique. Bis-GMA and TEG-DMA molar concentrations at various positions across the resin/dentin interfaces formed by EW and WW were measured using micro-Raman spectroscopy. The experiment was repeated at the same positions after 7-month storage in phosphate buffer solution containing 0.1% sodium azide. The μTBS and hybrid layer morphology (TEM) of bonding groups with and without chlorhexidine application were compared immediately and after 1-year storage in terms of nanoleakage, collagen fibril diameter, collagen interfibrillar width, and hybrid layer thickness. Results: Specimens bonded with EW showed significantly higher monomer molar concentrations and μTBS throughout the hybrid layer immediately and after storage, providing direct evidence of superior infiltration of hydrophobic monomers in EW compared to WW. Microscopically, EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. The application of chlorhexidine further preserved collagen integrity and limited the degree of nanoleakage in EW after 1-year storage. Conclusion: EW enhances infiltration of hydrophobic monomers into demineralized dentin. The results suggest that a more durable resin-dentin bond may be achieved with combined usage of a clinically relevant EW and chlorhexidine.
KW - Adhesives
KW - Bonding
KW - Collagen(s)
KW - Dentin
KW - Matrix metalloproteinases (MMPs)
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=84922394115&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922394115&partnerID=8YFLogxK
U2 - 10.3290/j.jad.a32695
DO - 10.3290/j.jad.a32695
M3 - Article
C2 - 25202747
AN - SCOPUS:84922394115
VL - 16
SP - 441
EP - 450
JO - The journal of adhesive dentistry
JF - The journal of adhesive dentistry
SN - 1461-5185
IS - 5
ER -