Ethanol-wet bonding and chlorhexidine improve resin-dentin bond durability: Quantitative analysis using Raman spectroscopy

Supitcha Talungchit, Julie L.P. Jessop, Deborah S. Cobb, Fang Qian, Saulo Geraldeli, David Henry Pashley, Steven R. Armstrong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: To directly test the effectiveness of ethanol-wet bonding (EW) in improving monomer infiltration into demineralized dentin through quantitative measurement of bis-GMA and TEG-DMA molar concentrations within hybrid layers, and to comprehensively evaluate the effect of EW and chlorhexidine on durability of resin-dentin bonds compared to conventional water-wet bonding (WW). Materials and Methods: A three-step etch-and-rinse adhesive (70% bis-GMA/28.75%TEG-DMA) was applied to coronal dentin using a clinically relevant ethanol-wet bonding protocol (EW) or the conventional water-wet bonding (WW) technique. Bis-GMA and TEG-DMA molar concentrations at various positions across the resin/dentin interfaces formed by EW and WW were measured using micro-Raman spectroscopy. The experiment was repeated at the same positions after 7-month storage in phosphate buffer solution containing 0.1% sodium azide. The μTBS and hybrid layer morphology (TEM) of bonding groups with and without chlorhexidine application were compared immediately and after 1-year storage in terms of nanoleakage, collagen fibril diameter, collagen interfibrillar width, and hybrid layer thickness. Results: Specimens bonded with EW showed significantly higher monomer molar concentrations and μTBS throughout the hybrid layer immediately and after storage, providing direct evidence of superior infiltration of hydrophobic monomers in EW compared to WW. Microscopically, EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. The application of chlorhexidine further preserved collagen integrity and limited the degree of nanoleakage in EW after 1-year storage. Conclusion: EW enhances infiltration of hydrophobic monomers into demineralized dentin. The results suggest that a more durable resin-dentin bond may be achieved with combined usage of a clinically relevant EW and chlorhexidine.

Original languageEnglish (US)
Pages (from-to)441-450
Number of pages10
JournalJournal of Adhesive Dentistry
Volume16
Issue number5
DOIs
StatePublished - Jan 1 2014

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Chlorhexidine
Raman Spectrum Analysis
Dentin
Ethanol
Bisphenol A-Glycidyl Methacrylate
Collagen
Water
Sodium Azide
Adhesives
Buffers
Phosphates

Keywords

  • Adhesives
  • Bonding
  • Collagen(s)
  • Dentin
  • Matrix metalloproteinases (MMPs)
  • Ultrastructure

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics

Cite this

Talungchit, S., Jessop, J. L. P., Cobb, D. S., Qian, F., Geraldeli, S., Pashley, D. H., & Armstrong, S. R. (2014). Ethanol-wet bonding and chlorhexidine improve resin-dentin bond durability: Quantitative analysis using Raman spectroscopy. Journal of Adhesive Dentistry, 16(5), 441-450. https://doi.org/10.3290/j.jad.a32695

Ethanol-wet bonding and chlorhexidine improve resin-dentin bond durability : Quantitative analysis using Raman spectroscopy. / Talungchit, Supitcha; Jessop, Julie L.P.; Cobb, Deborah S.; Qian, Fang; Geraldeli, Saulo; Pashley, David Henry; Armstrong, Steven R.

In: Journal of Adhesive Dentistry, Vol. 16, No. 5, 01.01.2014, p. 441-450.

Research output: Contribution to journalArticle

Talungchit, S, Jessop, JLP, Cobb, DS, Qian, F, Geraldeli, S, Pashley, DH & Armstrong, SR 2014, 'Ethanol-wet bonding and chlorhexidine improve resin-dentin bond durability: Quantitative analysis using Raman spectroscopy', Journal of Adhesive Dentistry, vol. 16, no. 5, pp. 441-450. https://doi.org/10.3290/j.jad.a32695
Talungchit, Supitcha ; Jessop, Julie L.P. ; Cobb, Deborah S. ; Qian, Fang ; Geraldeli, Saulo ; Pashley, David Henry ; Armstrong, Steven R. / Ethanol-wet bonding and chlorhexidine improve resin-dentin bond durability : Quantitative analysis using Raman spectroscopy. In: Journal of Adhesive Dentistry. 2014 ; Vol. 16, No. 5. pp. 441-450.
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abstract = "Purpose: To directly test the effectiveness of ethanol-wet bonding (EW) in improving monomer infiltration into demineralized dentin through quantitative measurement of bis-GMA and TEG-DMA molar concentrations within hybrid layers, and to comprehensively evaluate the effect of EW and chlorhexidine on durability of resin-dentin bonds compared to conventional water-wet bonding (WW). Materials and Methods: A three-step etch-and-rinse adhesive (70{\%} bis-GMA/28.75{\%}TEG-DMA) was applied to coronal dentin using a clinically relevant ethanol-wet bonding protocol (EW) or the conventional water-wet bonding (WW) technique. Bis-GMA and TEG-DMA molar concentrations at various positions across the resin/dentin interfaces formed by EW and WW were measured using micro-Raman spectroscopy. The experiment was repeated at the same positions after 7-month storage in phosphate buffer solution containing 0.1{\%} sodium azide. The μTBS and hybrid layer morphology (TEM) of bonding groups with and without chlorhexidine application were compared immediately and after 1-year storage in terms of nanoleakage, collagen fibril diameter, collagen interfibrillar width, and hybrid layer thickness. Results: Specimens bonded with EW showed significantly higher monomer molar concentrations and μTBS throughout the hybrid layer immediately and after storage, providing direct evidence of superior infiltration of hydrophobic monomers in EW compared to WW. Microscopically, EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. The application of chlorhexidine further preserved collagen integrity and limited the degree of nanoleakage in EW after 1-year storage. Conclusion: EW enhances infiltration of hydrophobic monomers into demineralized dentin. The results suggest that a more durable resin-dentin bond may be achieved with combined usage of a clinically relevant EW and chlorhexidine.",
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AU - Geraldeli, Saulo

AU - Pashley, David Henry

AU - Armstrong, Steven R.

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AB - Purpose: To directly test the effectiveness of ethanol-wet bonding (EW) in improving monomer infiltration into demineralized dentin through quantitative measurement of bis-GMA and TEG-DMA molar concentrations within hybrid layers, and to comprehensively evaluate the effect of EW and chlorhexidine on durability of resin-dentin bonds compared to conventional water-wet bonding (WW). Materials and Methods: A three-step etch-and-rinse adhesive (70% bis-GMA/28.75%TEG-DMA) was applied to coronal dentin using a clinically relevant ethanol-wet bonding protocol (EW) or the conventional water-wet bonding (WW) technique. Bis-GMA and TEG-DMA molar concentrations at various positions across the resin/dentin interfaces formed by EW and WW were measured using micro-Raman spectroscopy. The experiment was repeated at the same positions after 7-month storage in phosphate buffer solution containing 0.1% sodium azide. The μTBS and hybrid layer morphology (TEM) of bonding groups with and without chlorhexidine application were compared immediately and after 1-year storage in terms of nanoleakage, collagen fibril diameter, collagen interfibrillar width, and hybrid layer thickness. Results: Specimens bonded with EW showed significantly higher monomer molar concentrations and μTBS throughout the hybrid layer immediately and after storage, providing direct evidence of superior infiltration of hydrophobic monomers in EW compared to WW. Microscopically, EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. The application of chlorhexidine further preserved collagen integrity and limited the degree of nanoleakage in EW after 1-year storage. Conclusion: EW enhances infiltration of hydrophobic monomers into demineralized dentin. The results suggest that a more durable resin-dentin bond may be achieved with combined usage of a clinically relevant EW and chlorhexidine.

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