Ethanol wet-bonding technique sensitivity assessed by AFM

E. Osorio, M. Toledano, F. S. Aguilera, Franklin Chi Meng Tay, R. Osorio

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In ethanol wet bonding, water is replaced by ethanol to maintain dehydrated collagen matrices in an extended state to facilitate resin infiltration. Since short ethanol dehydration protocols may be ineffective, this study tested the null hypothesis that there are no differences in ethanol dehydration protocols for maintaining the surface roughness, fibril diameter, and interfibrillar spaces of acid-etched dentin. Polished human dentin surfaces were etched with phosphoric acid and water-rinsed. Tested protocols were: (1) water-rinse (control); (2) 100% ethanol-rinse (1-min); (3) 100% ethanol-rinse (5-min); and (4) progressive ethanol replacement (50-100%). Surface roughness, fibril diameter, and interfibrillar spaces were determined with atomic force microscopy and analyzed by one-way analysis of variance and the Student-Newman-Keuls test (α = 0.05). Dentin roughness and fibril diameter significantly decreased when 100% ethanol (1-5 min) was used for rinsing (p < 0.001). Absolute ethanol produced collapse and shrinkage of collagen fibrils. Ascending ethanol concentrations did not collapse the matrix and shrank the fibrils less than absolute ethanol-rinses.

Original languageEnglish (US)
Pages (from-to)1264-1269
Number of pages6
JournalJournal of Dental Research
Volume89
Issue number11
DOIs
StatePublished - Nov 1 2010

Fingerprint

Ethanol
Dentin
Dehydration
Water
Collagen
Atomic Force Microscopy
Analysis of Variance
Students
Acids

Keywords

  • acid-etched dentin
  • atomic force microscopy
  • ethanol-saturated
  • surface topography
  • water replacement

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Ethanol wet-bonding technique sensitivity assessed by AFM. / Osorio, E.; Toledano, M.; Aguilera, F. S.; Tay, Franklin Chi Meng; Osorio, R.

In: Journal of Dental Research, Vol. 89, No. 11, 01.11.2010, p. 1264-1269.

Research output: Contribution to journalArticle

Osorio, E, Toledano, M, Aguilera, FS, Tay, FCM & Osorio, R 2010, 'Ethanol wet-bonding technique sensitivity assessed by AFM', Journal of Dental Research, vol. 89, no. 11, pp. 1264-1269. https://doi.org/10.1177/0022034510376403
Osorio, E. ; Toledano, M. ; Aguilera, F. S. ; Tay, Franklin Chi Meng ; Osorio, R. / Ethanol wet-bonding technique sensitivity assessed by AFM. In: Journal of Dental Research. 2010 ; Vol. 89, No. 11. pp. 1264-1269.
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