Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

Mahmut Kusdemir, Solen Gunal, Fusun Ozer, Satoshi Imazato, Naomi Izutani, Shigeyuki Ebisu, Markus B. Blatz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study evaluated the cytotoxicity of self-etching primers/adhesives by direct contact and dentin barrier tests. The three two-step self-etching systems Clearfl SE Bond (CSE), Clearfl Protect Bond (CPB), Prime&Bond NT/NRC (PB) and one-step self-etching systems Reactmer Bond (RB), Clearfl Tri-S Bond (CTS), and Adper Prompt L-Pop (AP) were examined. In direct contact tests, L929 cells were cultured in the presence of diluted solutions (50, 20, 10, and 1%) of primer/conditioner of adhesive systems. For dentin barrier tests, each system was applied onto 0.5 or 1.5 mm thick human dentin assembled in a simple pulp chamber device and incubated for 24 h at 37°C to make the diffusive components contact the L929 cells placed at the bottom of the chamber. The cytotoxic effects were assessed by MTT assay. Cell culture without application of any primers/adhesives served as the control for both tests. One-way ANOVA and Tukey HSD tests were used for statistical analyses. The direct contact tests demonstrated that CSE and CPB were less toxic than the other materials at all dilutions. In the dentin barrier tests, toxic effects of materials were reduced with an increase in thickness of intervening dentin. CSE and CPB showed less cytotoxicity than the other adhesives (p<0.05) when applied to 0.5 mm-thick dentin, and CSE was the least toxic in the 1.5 mm-dentin group (p<0.05). Dentin thickness positively affected biocompatibility of the tested bonding systems. Two-step self-etching systems with HEMA-based primers were more biocompatible than other self-etching adhesives.

Original languageEnglish (US)
Pages (from-to)799-805
Number of pages7
JournalDental Materials Journal
Volume30
Issue number6
DOIs
StatePublished - Dec 9 2011

Fingerprint

Dentin
Adhesives
Etching
Poisons
Cytotoxicity
Analysis of variance (ANOVA)
Biocompatibility
Cell culture
Dilution
Pulp
Assays
Dental Pulp Cavity
Cultured Cells
Analysis of Variance
Cell Culture Techniques
Clearfil Protect Bond
Equipment and Supplies

Keywords

  • Cytotoxicity
  • MTT assay
  • Self-etching adhesives

ASJC Scopus subject areas

  • Ceramics and Composites
  • Dentistry(all)

Cite this

Kusdemir, M., Gunal, S., Ozer, F., Imazato, S., Izutani, N., Ebisu, S., & Blatz, M. B. (2011). Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests. Dental Materials Journal, 30(6), 799-805. https://doi.org/10.4012/dmj.2011-046

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests. / Kusdemir, Mahmut; Gunal, Solen; Ozer, Fusun; Imazato, Satoshi; Izutani, Naomi; Ebisu, Shigeyuki; Blatz, Markus B.

In: Dental Materials Journal, Vol. 30, No. 6, 09.12.2011, p. 799-805.

Research output: Contribution to journalArticle

Kusdemir, M, Gunal, S, Ozer, F, Imazato, S, Izutani, N, Ebisu, S & Blatz, MB 2011, 'Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests', Dental Materials Journal, vol. 30, no. 6, pp. 799-805. https://doi.org/10.4012/dmj.2011-046
Kusdemir, Mahmut ; Gunal, Solen ; Ozer, Fusun ; Imazato, Satoshi ; Izutani, Naomi ; Ebisu, Shigeyuki ; Blatz, Markus B. / Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests. In: Dental Materials Journal. 2011 ; Vol. 30, No. 6. pp. 799-805.
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