Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease

Julie V. Philley, Anbarasu Kannan, David E. Griffith, Megan S. Devine, Jeana L. Benwill, Richard J. Wallace, Barbara A. Brown-Elliott, Foram Thakkar, Varsha Surendranath Taskar, James G. Fox, Ammar Alqaid, Hernaina Bains, Sudeep Gupta, Santanu Dasgupta

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bronchiectasis Nontuberculous mycobacterium (NTMnb) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTMnb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa- NTMnb cases. Co-culture of ECM1+ exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production. Co-culture of the ECM1+ exosomes with normal human T cells modulated their cytokine production. The ECM1+ exosomes were markedly higher in sera obtained from BCa-NTMnb subjects. Exclusive expression of APN, APOC4 and AZGP1 was evident in the circulating exosomes of these BCa-NTMnb cases, which predicts disease prevalence independent of the body max index in concert with ECM1. Monitoring ECM1, APN, APOC4 and AZGP1 in the circulating exosomes could be beneficial for risk assessment, monitoring and surveillance of BCa-NTMnb.

Original languageEnglish (US)
Pages (from-to)18070-18081
Number of pages12
JournalOncotarget
Volume8
Issue number11
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

Exosomes
Extracellular Matrix Proteins
Breast Neoplasms
Coculture Techniques
Nontuberculous Mycobacterium Infections
Bronchiectasis
Epithelial-Mesenchymal Transition
Vimentin
Proteome
Serum
Glutamic Acid
Mass Spectrometry
Breast
Epithelial Cells
Cytokines
T-Lymphocytes
Health

Keywords

  • Biomarker
  • Breast cancer
  • ECM1
  • Exosomes
  • Nontuberculous Mycobacterium

ASJC Scopus subject areas

  • Oncology

Cite this

Philley, J. V., Kannan, A., Griffith, D. E., Devine, M. S., Benwill, J. L., Wallace, R. J., ... Dasgupta, S. (2017). Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. Oncotarget, 8(11), 18070-18081. https://doi.org/10.18632/oncotarget.14964

Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. / Philley, Julie V.; Kannan, Anbarasu; Griffith, David E.; Devine, Megan S.; Benwill, Jeana L.; Wallace, Richard J.; Brown-Elliott, Barbara A.; Thakkar, Foram; Taskar, Varsha Surendranath; Fox, James G.; Alqaid, Ammar; Bains, Hernaina; Gupta, Sudeep; Dasgupta, Santanu.

In: Oncotarget, Vol. 8, No. 11, 01.01.2017, p. 18070-18081.

Research output: Contribution to journalArticle

Philley, JV, Kannan, A, Griffith, DE, Devine, MS, Benwill, JL, Wallace, RJ, Brown-Elliott, BA, Thakkar, F, Taskar, VS, Fox, JG, Alqaid, A, Bains, H, Gupta, S & Dasgupta, S 2017, 'Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease', Oncotarget, vol. 8, no. 11, pp. 18070-18081. https://doi.org/10.18632/oncotarget.14964
Philley JV, Kannan A, Griffith DE, Devine MS, Benwill JL, Wallace RJ et al. Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. Oncotarget. 2017 Jan 1;8(11):18070-18081. https://doi.org/10.18632/oncotarget.14964
Philley, Julie V. ; Kannan, Anbarasu ; Griffith, David E. ; Devine, Megan S. ; Benwill, Jeana L. ; Wallace, Richard J. ; Brown-Elliott, Barbara A. ; Thakkar, Foram ; Taskar, Varsha Surendranath ; Fox, James G. ; Alqaid, Ammar ; Bains, Hernaina ; Gupta, Sudeep ; Dasgupta, Santanu. / Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. In: Oncotarget. 2017 ; Vol. 8, No. 11. pp. 18070-18081.
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