Expression and cyclic variations of catechol-O-methyl transferase in human endometrial stroma

Sana M. Salih, Salama A. Salama, Amin A. Fadl, Manubai Nagamani, Ayman Al-Hendy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To investigate the role of catechol-O-methyl transferase (COMT) in the regulation of estrogen metabolism in human endometrium. Design: Laboratory study. Setting: Academic research laboratory. Intervention(s): Immunohistochemistry was used to localize COMT protein in human endometrial tissues. Catechol-O-methyl transferase promoter-luciferace reporter gene transactivation assay was used to assess COMT promoter activity in response to estrogen and progesterone treatment in primary human endometrial stroma (pHES) cells. Catechol-O-methyl transferase protein and mRNA expression were determined by Western blot and/or real-time polymerase chain reaction. The effect of 2-methoxy estrogen treatment on DNA proliferation, B-cell lymphoma 2, and vascular epithelial growth factor protein expression were assessed by Hoechst and Western blot analyses, respectively. Main Outcome Measure(s): Catechol-O-methyl transferase protein and mRNA subcellular localization and expression in human endometrial tissues and pHES cells. Result(s): Catechol-O-methyl transferase protein expression in human endometrial tissues was up-regulated in the proliferative phase and down-regulated in the midsecretory phase of the menstrual cycle. Estrogen induced a dose-dependent increase in COMT proximal promotor-luciferace transactivation in pHES cells whereas progesterone inhibited it. Estrogen up-regulated soluble COMT protein isoform expression whereas the addition of progesterone down-regulated it in pHES cells. High doses of 2-methoxy estrogen inhibited endometrial stroma cell proliferation, and down-regulated B-cell lymphoma 2 and vascular epithelial growth factor protein expression. Conclusion(s): Catechol-O-methyl transferase expression is hormonally regulated in human endometrial stroma. Catechol-O-methyl transferase product, 2-methoxy estrogen, inhibited endometrial stroma cell proliferation and decreased vascular epithelial growth factor and B-cell lymphoma 2 protein expression.

Original languageEnglish (US)
Pages (from-to)789-797
Number of pages9
JournalFertility and Sterility
Volume90
Issue number3
DOIs
StatePublished - Sep 1 2008

Fingerprint

Guaiacol
Transferases
Estrogens
B-Cell Lymphoma
Proteins
Progesterone
Blood Vessels
Intercellular Signaling Peptides and Proteins
Transcriptional Activation
Western Blotting
Cell Proliferation
Messenger RNA
Menstrual Cycle
Endometrium
Reporter Genes
Real-Time Polymerase Chain Reaction
Protein Isoforms
Immunohistochemistry

Keywords

  • 2-methoxy estrogen
  • catechol estrogen
  • Catechol-O-methyl transferase
  • human endometrial stroma

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Expression and cyclic variations of catechol-O-methyl transferase in human endometrial stroma. / Salih, Sana M.; Salama, Salama A.; Fadl, Amin A.; Nagamani, Manubai; Al-Hendy, Ayman.

In: Fertility and Sterility, Vol. 90, No. 3, 01.09.2008, p. 789-797.

Research output: Contribution to journalArticle

Salih, Sana M. ; Salama, Salama A. ; Fadl, Amin A. ; Nagamani, Manubai ; Al-Hendy, Ayman. / Expression and cyclic variations of catechol-O-methyl transferase in human endometrial stroma. In: Fertility and Sterility. 2008 ; Vol. 90, No. 3. pp. 789-797.
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abstract = "Objective: To investigate the role of catechol-O-methyl transferase (COMT) in the regulation of estrogen metabolism in human endometrium. Design: Laboratory study. Setting: Academic research laboratory. Intervention(s): Immunohistochemistry was used to localize COMT protein in human endometrial tissues. Catechol-O-methyl transferase promoter-luciferace reporter gene transactivation assay was used to assess COMT promoter activity in response to estrogen and progesterone treatment in primary human endometrial stroma (pHES) cells. Catechol-O-methyl transferase protein and mRNA expression were determined by Western blot and/or real-time polymerase chain reaction. The effect of 2-methoxy estrogen treatment on DNA proliferation, B-cell lymphoma 2, and vascular epithelial growth factor protein expression were assessed by Hoechst and Western blot analyses, respectively. Main Outcome Measure(s): Catechol-O-methyl transferase protein and mRNA subcellular localization and expression in human endometrial tissues and pHES cells. Result(s): Catechol-O-methyl transferase protein expression in human endometrial tissues was up-regulated in the proliferative phase and down-regulated in the midsecretory phase of the menstrual cycle. Estrogen induced a dose-dependent increase in COMT proximal promotor-luciferace transactivation in pHES cells whereas progesterone inhibited it. Estrogen up-regulated soluble COMT protein isoform expression whereas the addition of progesterone down-regulated it in pHES cells. High doses of 2-methoxy estrogen inhibited endometrial stroma cell proliferation, and down-regulated B-cell lymphoma 2 and vascular epithelial growth factor protein expression. Conclusion(s): Catechol-O-methyl transferase expression is hormonally regulated in human endometrial stroma. Catechol-O-methyl transferase product, 2-methoxy estrogen, inhibited endometrial stroma cell proliferation and decreased vascular epithelial growth factor and B-cell lymphoma 2 protein expression.",
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AU - Salih, Sana M.

AU - Salama, Salama A.

AU - Fadl, Amin A.

AU - Nagamani, Manubai

AU - Al-Hendy, Ayman

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N2 - Objective: To investigate the role of catechol-O-methyl transferase (COMT) in the regulation of estrogen metabolism in human endometrium. Design: Laboratory study. Setting: Academic research laboratory. Intervention(s): Immunohistochemistry was used to localize COMT protein in human endometrial tissues. Catechol-O-methyl transferase promoter-luciferace reporter gene transactivation assay was used to assess COMT promoter activity in response to estrogen and progesterone treatment in primary human endometrial stroma (pHES) cells. Catechol-O-methyl transferase protein and mRNA expression were determined by Western blot and/or real-time polymerase chain reaction. The effect of 2-methoxy estrogen treatment on DNA proliferation, B-cell lymphoma 2, and vascular epithelial growth factor protein expression were assessed by Hoechst and Western blot analyses, respectively. Main Outcome Measure(s): Catechol-O-methyl transferase protein and mRNA subcellular localization and expression in human endometrial tissues and pHES cells. Result(s): Catechol-O-methyl transferase protein expression in human endometrial tissues was up-regulated in the proliferative phase and down-regulated in the midsecretory phase of the menstrual cycle. Estrogen induced a dose-dependent increase in COMT proximal promotor-luciferace transactivation in pHES cells whereas progesterone inhibited it. Estrogen up-regulated soluble COMT protein isoform expression whereas the addition of progesterone down-regulated it in pHES cells. High doses of 2-methoxy estrogen inhibited endometrial stroma cell proliferation, and down-regulated B-cell lymphoma 2 and vascular epithelial growth factor protein expression. Conclusion(s): Catechol-O-methyl transferase expression is hormonally regulated in human endometrial stroma. Catechol-O-methyl transferase product, 2-methoxy estrogen, inhibited endometrial stroma cell proliferation and decreased vascular epithelial growth factor and B-cell lymphoma 2 protein expression.

AB - Objective: To investigate the role of catechol-O-methyl transferase (COMT) in the regulation of estrogen metabolism in human endometrium. Design: Laboratory study. Setting: Academic research laboratory. Intervention(s): Immunohistochemistry was used to localize COMT protein in human endometrial tissues. Catechol-O-methyl transferase promoter-luciferace reporter gene transactivation assay was used to assess COMT promoter activity in response to estrogen and progesterone treatment in primary human endometrial stroma (pHES) cells. Catechol-O-methyl transferase protein and mRNA expression were determined by Western blot and/or real-time polymerase chain reaction. The effect of 2-methoxy estrogen treatment on DNA proliferation, B-cell lymphoma 2, and vascular epithelial growth factor protein expression were assessed by Hoechst and Western blot analyses, respectively. Main Outcome Measure(s): Catechol-O-methyl transferase protein and mRNA subcellular localization and expression in human endometrial tissues and pHES cells. Result(s): Catechol-O-methyl transferase protein expression in human endometrial tissues was up-regulated in the proliferative phase and down-regulated in the midsecretory phase of the menstrual cycle. Estrogen induced a dose-dependent increase in COMT proximal promotor-luciferace transactivation in pHES cells whereas progesterone inhibited it. Estrogen up-regulated soluble COMT protein isoform expression whereas the addition of progesterone down-regulated it in pHES cells. High doses of 2-methoxy estrogen inhibited endometrial stroma cell proliferation, and down-regulated B-cell lymphoma 2 and vascular epithelial growth factor protein expression. Conclusion(s): Catechol-O-methyl transferase expression is hormonally regulated in human endometrial stroma. Catechol-O-methyl transferase product, 2-methoxy estrogen, inhibited endometrial stroma cell proliferation and decreased vascular epithelial growth factor and B-cell lymphoma 2 protein expression.

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