Expression and purification of ZNF191(243-368) in three expression systems

Dong Xin Zhao, Xin Cheng Teng, Zhi Chun Ding, Zhong Xian Huang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

ZNF191(243-368), a new human zinc finger protein, probably relates to some hereditary diseases and cancers. To obtain adequate amount of ZNF191(243-368) for the study of its property, structure and function, three different expression systems of inclusion-body, glutathione S-transferase (GST), and hexahistidine (6 X His) were used and compared. Among these systems, the expression level of ZNF191(243-368) was increased in inclusion body system under a higher isopropylthio-β-D-galactoside (IPTG) concentration, but the non-target proteins were also increased more, which made its purification more difficult and the yield lower. The expression of His-tag fusion protein was almost not affected by IPTG concentration, temperature and inducing time. At a high IPTG concentration the highest expression yield for GST fusion protein was obtained. And the fusion proteins can be partially purified by a single affinity chromatography step. The fusion protein systems show advantages for expression of these proteins.

Original languageEnglish (US)
Pages (from-to)1739-1742
Number of pages4
JournalChinese Journal of Chemistry
Volume25
Issue number11
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

Keywords

  • Affinity chromatography
  • Fusion protein
  • Isopropylthio-β-D-galactoside
  • Protein expression

ASJC Scopus subject areas

  • Chemistry(all)

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