Fatty acid and long‐chain base composition of gangliosides isolated from adult human brain

S. Ando, Robert K Yu

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophlic composition. Hematosides, GM3 and GD3, each showing double bands on thin‐layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity of their ceramide moieties. The upper band fractions of GM3 and GD3 were found to contain relatively high amounts of longer‐chain fatty acids (C20C26), whereas the lower band fractions contained high amounts of shorterchain fatty acids (C16C18). Compared to other gangliosides which contain hexosamine, the two hematosides contained a smaller proportion of stearic acid. The proportion of 4‐eicosasphingenine was found to increase with increasing sialic acid content in gangliosides. These findings suggest that, in the biosynthesis of various gangliosides, N‐acetylhexosamine is preferentially transferred to hematosides rich in stearic acid, and sialic acid residues are preferentially transfered to 4‐eicosasphingenine‐containing species.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalJournal of Neuroscience Research
Volume12
Issue number2-3
DOIs
Publication statusPublished - 1984
Externally publishedYes

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Keywords

  • fatty acids
  • gangliosides
  • human brain
  • long‐chain bases

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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