Fatty acid and long‐chain base composition of gangliosides isolated from adult human brain

S. Ando, Robert K Yu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophlic composition. Hematosides, GM3 and GD3, each showing double bands on thin‐layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity of their ceramide moieties. The upper band fractions of GM3 and GD3 were found to contain relatively high amounts of longer‐chain fatty acids (C20C26), whereas the lower band fractions contained high amounts of shorterchain fatty acids (C16C18). Compared to other gangliosides which contain hexosamine, the two hematosides contained a smaller proportion of stearic acid. The proportion of 4‐eicosasphingenine was found to increase with increasing sialic acid content in gangliosides. These findings suggest that, in the biosynthesis of various gangliosides, N‐acetylhexosamine is preferentially transferred to hematosides rich in stearic acid, and sialic acid residues are preferentially transfered to 4‐eicosasphingenine‐containing species.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalJournal of Neuroscience Research
Volume12
Issue number2-3
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Gangliosides
G(M3) Ganglioside
Base Composition
Fatty Acids
Brain
N-Acetylneuraminic Acid
Hexosamines
Ceramides
stearic acid

Keywords

  • fatty acids
  • gangliosides
  • human brain
  • long‐chain bases

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Fatty acid and long‐chain base composition of gangliosides isolated from adult human brain. / Ando, S.; Yu, Robert K.

In: Journal of Neuroscience Research, Vol. 12, No. 2-3, 01.01.1984, p. 205-211.

Research output: Contribution to journalArticle

@article{0712067771e74bd58d7a7228c2b86a99,
title = "Fatty acid and long‐chain base composition of gangliosides isolated from adult human brain",
abstract = "A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophlic composition. Hematosides, GM3 and GD3, each showing double bands on thin‐layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity of their ceramide moieties. The upper band fractions of GM3 and GD3 were found to contain relatively high amounts of longer‐chain fatty acids (C20C26), whereas the lower band fractions contained high amounts of shorterchain fatty acids (C16C18). Compared to other gangliosides which contain hexosamine, the two hematosides contained a smaller proportion of stearic acid. The proportion of 4‐eicosasphingenine was found to increase with increasing sialic acid content in gangliosides. These findings suggest that, in the biosynthesis of various gangliosides, N‐acetylhexosamine is preferentially transferred to hematosides rich in stearic acid, and sialic acid residues are preferentially transfered to 4‐eicosasphingenine‐containing species.",
keywords = "fatty acids, gangliosides, human brain, long‐chain bases",
author = "S. Ando and Yu, {Robert K}",
year = "1984",
month = "1",
day = "1",
doi = "10.1002/jnr.490120208",
language = "English (US)",
volume = "12",
pages = "205--211",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "2-3",

}

TY - JOUR

T1 - Fatty acid and long‐chain base composition of gangliosides isolated from adult human brain

AU - Ando, S.

AU - Yu, Robert K

PY - 1984/1/1

Y1 - 1984/1/1

N2 - A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophlic composition. Hematosides, GM3 and GD3, each showing double bands on thin‐layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity of their ceramide moieties. The upper band fractions of GM3 and GD3 were found to contain relatively high amounts of longer‐chain fatty acids (C20C26), whereas the lower band fractions contained high amounts of shorterchain fatty acids (C16C18). Compared to other gangliosides which contain hexosamine, the two hematosides contained a smaller proportion of stearic acid. The proportion of 4‐eicosasphingenine was found to increase with increasing sialic acid content in gangliosides. These findings suggest that, in the biosynthesis of various gangliosides, N‐acetylhexosamine is preferentially transferred to hematosides rich in stearic acid, and sialic acid residues are preferentially transfered to 4‐eicosasphingenine‐containing species.

AB - A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophlic composition. Hematosides, GM3 and GD3, each showing double bands on thin‐layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity of their ceramide moieties. The upper band fractions of GM3 and GD3 were found to contain relatively high amounts of longer‐chain fatty acids (C20C26), whereas the lower band fractions contained high amounts of shorterchain fatty acids (C16C18). Compared to other gangliosides which contain hexosamine, the two hematosides contained a smaller proportion of stearic acid. The proportion of 4‐eicosasphingenine was found to increase with increasing sialic acid content in gangliosides. These findings suggest that, in the biosynthesis of various gangliosides, N‐acetylhexosamine is preferentially transferred to hematosides rich in stearic acid, and sialic acid residues are preferentially transfered to 4‐eicosasphingenine‐containing species.

KW - fatty acids

KW - gangliosides

KW - human brain

KW - long‐chain bases

UR - http://www.scopus.com/inward/record.url?scp=0021150289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021150289&partnerID=8YFLogxK

U2 - 10.1002/jnr.490120208

DO - 10.1002/jnr.490120208

M3 - Article

VL - 12

SP - 205

EP - 211

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 2-3

ER -