Fluorescence detected magnetic circular dichroism of fluorescent and nonfluorescent molecules

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Abstract

We have modified a spectrometer for the measurement of fluorescence detected magnetic (and natural) circular dichroism (FDMCD). The instrument can be operated either in a direct mode in wich the average and polarization induced differential fluorescent intensities are recorded separately or in a mode which records their ratio. We have measured the FDMCD of tryptophan, which is itself fluorescent. With the aid of an added fluorescent molecule we also measured the FDMCD of Fe (III) cytochrome c, which is nonfluorescent. In both cases, the FDMCD agreed with the conventional transmission detected magnetic circular dichroism within experimental uncertainty. The FDMCD of both fluorescent molecules is potentially a useful technique for investigating optically dense materials and biological molecules in their native environments and systems in which there is inter or intramolecular energy transfer.

Original languageEnglish (US)
Pages (from-to)276-280
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number2
DOIs
StatePublished - Jan 1 1976

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Circular Dichroism
Fluorescence
Energy Transfer
Cytochromes c
Tryptophan
Uncertainty

ASJC Scopus subject areas

  • General

Cite this

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title = "Fluorescence detected magnetic circular dichroism of fluorescent and nonfluorescent molecules",
abstract = "We have modified a spectrometer for the measurement of fluorescence detected magnetic (and natural) circular dichroism (FDMCD). The instrument can be operated either in a direct mode in wich the average and polarization induced differential fluorescent intensities are recorded separately or in a mode which records their ratio. We have measured the FDMCD of tryptophan, which is itself fluorescent. With the aid of an added fluorescent molecule we also measured the FDMCD of Fe (III) cytochrome c, which is nonfluorescent. In both cases, the FDMCD agreed with the conventional transmission detected magnetic circular dichroism within experimental uncertainty. The FDMCD of both fluorescent molecules is potentially a useful technique for investigating optically dense materials and biological molecules in their native environments and systems in which there is inter or intramolecular energy transfer.",
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AB - We have modified a spectrometer for the measurement of fluorescence detected magnetic (and natural) circular dichroism (FDMCD). The instrument can be operated either in a direct mode in wich the average and polarization induced differential fluorescent intensities are recorded separately or in a mode which records their ratio. We have measured the FDMCD of tryptophan, which is itself fluorescent. With the aid of an added fluorescent molecule we also measured the FDMCD of Fe (III) cytochrome c, which is nonfluorescent. In both cases, the FDMCD agreed with the conventional transmission detected magnetic circular dichroism within experimental uncertainty. The FDMCD of both fluorescent molecules is potentially a useful technique for investigating optically dense materials and biological molecules in their native environments and systems in which there is inter or intramolecular energy transfer.

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