Functional recovery following nerve injury and repair by silicon tubulization: Comparison of laminin-fibronectin, dialyzed plasma, collagen gel, and phosphate buffered solution

David J Terris, Elbert T. Cheng, David S. Utley, Derjung M. Tarn, Pei Ran Ho, A. Neil Verity

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Purpose: This study was designed to investigate the potential for enhancement of peripheral nerve regeneration by the manipulation of the neural microenvironment with laminin-fibronectin solution (LF), dialyzed plasma (DP), collagen gel (CG), or phosphate buffered saline (PBS) in a silicon tubulization repair model. Method: A rat sciatic nerve model of injury and repair was used to study the effects of exogenous matrix precursors (contained in LF or DP), CG or PBS on nerve regeneration. A total of 50 Sprague-Dawley rats underwent left sciatic nerve transection and repair by silicon tubulization. The silicon tubules were either left empty (E), or filled with solutions of LF, DP, CG, or PBS. Nerve function was assessed preoperatively and then postoperatively, every 10 days for 90 days using sciatic functional indexes (SFI). On postoperative day 90, the sciatic nerves were harvested for histologic analysis and the posterior compartment muscles of each animal were harvested and weighed. Molecular analysis for two proteins associated with neural regeneration was performed on the nerve segments. Results: All five animal groups demonstrated equivalent functional recovery. Comparison of the rate of recovery and mean maximal recovery between each group revealed no statistically significant differences, with P- values ranging from 0.30 to 0.95. Posterior compartment muscle masses were similar in all groups except for LF, whose animals had muscle masses 8-9% lower than CG, PBS, or E (P<0.05). Conclusion: Alteration of the regenerating neural microenvironment with exogenous matrix precursors (LF, DP), CG or PBS failed to improve sciatic functional recovery after nerve transection and silicon tubulization in this model. From this study, we conclude that LF, DP, CG, and PBS do not enhance the rate or degree of recovery of peripheral nerve function across a narrow gap when nerves are repaired by silicon tubulization.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalAuris Nasus Larynx
Volume26
Issue number2
DOIs
StatePublished - Apr 1 1999
Externally publishedYes

Fingerprint

Laminin
Silicon
Fibronectins
Collagen
Gels
Phosphates
Wounds and Injuries
Sciatic Nerve
Nerve Regeneration
Peripheral Nerves
Muscles
Sprague Dawley Rats
Regeneration
Proteins

Keywords

  • Peripheral nerve regeneration
  • Sciatic functional indexes
  • Silicon tubulization repair model

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

Functional recovery following nerve injury and repair by silicon tubulization : Comparison of laminin-fibronectin, dialyzed plasma, collagen gel, and phosphate buffered solution. / Terris, David J; Cheng, Elbert T.; Utley, David S.; Tarn, Derjung M.; Ho, Pei Ran; Verity, A. Neil.

In: Auris Nasus Larynx, Vol. 26, No. 2, 01.04.1999, p. 117-122.

Research output: Contribution to journalArticle

@article{b380976a9ea1414fb7582c77b6fe2098,
title = "Functional recovery following nerve injury and repair by silicon tubulization: Comparison of laminin-fibronectin, dialyzed plasma, collagen gel, and phosphate buffered solution",
abstract = "Purpose: This study was designed to investigate the potential for enhancement of peripheral nerve regeneration by the manipulation of the neural microenvironment with laminin-fibronectin solution (LF), dialyzed plasma (DP), collagen gel (CG), or phosphate buffered saline (PBS) in a silicon tubulization repair model. Method: A rat sciatic nerve model of injury and repair was used to study the effects of exogenous matrix precursors (contained in LF or DP), CG or PBS on nerve regeneration. A total of 50 Sprague-Dawley rats underwent left sciatic nerve transection and repair by silicon tubulization. The silicon tubules were either left empty (E), or filled with solutions of LF, DP, CG, or PBS. Nerve function was assessed preoperatively and then postoperatively, every 10 days for 90 days using sciatic functional indexes (SFI). On postoperative day 90, the sciatic nerves were harvested for histologic analysis and the posterior compartment muscles of each animal were harvested and weighed. Molecular analysis for two proteins associated with neural regeneration was performed on the nerve segments. Results: All five animal groups demonstrated equivalent functional recovery. Comparison of the rate of recovery and mean maximal recovery between each group revealed no statistically significant differences, with P- values ranging from 0.30 to 0.95. Posterior compartment muscle masses were similar in all groups except for LF, whose animals had muscle masses 8-9{\%} lower than CG, PBS, or E (P<0.05). Conclusion: Alteration of the regenerating neural microenvironment with exogenous matrix precursors (LF, DP), CG or PBS failed to improve sciatic functional recovery after nerve transection and silicon tubulization in this model. From this study, we conclude that LF, DP, CG, and PBS do not enhance the rate or degree of recovery of peripheral nerve function across a narrow gap when nerves are repaired by silicon tubulization.",
keywords = "Peripheral nerve regeneration, Sciatic functional indexes, Silicon tubulization repair model",
author = "Terris, {David J} and Cheng, {Elbert T.} and Utley, {David S.} and Tarn, {Derjung M.} and Ho, {Pei Ran} and Verity, {A. Neil}",
year = "1999",
month = "4",
day = "1",
doi = "10.1016/S0385-8146(98)00067-4",
language = "English (US)",
volume = "26",
pages = "117--122",
journal = "Auris Nasus Larynx",
issn = "0385-8146",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Functional recovery following nerve injury and repair by silicon tubulization

T2 - Comparison of laminin-fibronectin, dialyzed plasma, collagen gel, and phosphate buffered solution

AU - Terris, David J

AU - Cheng, Elbert T.

AU - Utley, David S.

AU - Tarn, Derjung M.

AU - Ho, Pei Ran

AU - Verity, A. Neil

PY - 1999/4/1

Y1 - 1999/4/1

N2 - Purpose: This study was designed to investigate the potential for enhancement of peripheral nerve regeneration by the manipulation of the neural microenvironment with laminin-fibronectin solution (LF), dialyzed plasma (DP), collagen gel (CG), or phosphate buffered saline (PBS) in a silicon tubulization repair model. Method: A rat sciatic nerve model of injury and repair was used to study the effects of exogenous matrix precursors (contained in LF or DP), CG or PBS on nerve regeneration. A total of 50 Sprague-Dawley rats underwent left sciatic nerve transection and repair by silicon tubulization. The silicon tubules were either left empty (E), or filled with solutions of LF, DP, CG, or PBS. Nerve function was assessed preoperatively and then postoperatively, every 10 days for 90 days using sciatic functional indexes (SFI). On postoperative day 90, the sciatic nerves were harvested for histologic analysis and the posterior compartment muscles of each animal were harvested and weighed. Molecular analysis for two proteins associated with neural regeneration was performed on the nerve segments. Results: All five animal groups demonstrated equivalent functional recovery. Comparison of the rate of recovery and mean maximal recovery between each group revealed no statistically significant differences, with P- values ranging from 0.30 to 0.95. Posterior compartment muscle masses were similar in all groups except for LF, whose animals had muscle masses 8-9% lower than CG, PBS, or E (P<0.05). Conclusion: Alteration of the regenerating neural microenvironment with exogenous matrix precursors (LF, DP), CG or PBS failed to improve sciatic functional recovery after nerve transection and silicon tubulization in this model. From this study, we conclude that LF, DP, CG, and PBS do not enhance the rate or degree of recovery of peripheral nerve function across a narrow gap when nerves are repaired by silicon tubulization.

AB - Purpose: This study was designed to investigate the potential for enhancement of peripheral nerve regeneration by the manipulation of the neural microenvironment with laminin-fibronectin solution (LF), dialyzed plasma (DP), collagen gel (CG), or phosphate buffered saline (PBS) in a silicon tubulization repair model. Method: A rat sciatic nerve model of injury and repair was used to study the effects of exogenous matrix precursors (contained in LF or DP), CG or PBS on nerve regeneration. A total of 50 Sprague-Dawley rats underwent left sciatic nerve transection and repair by silicon tubulization. The silicon tubules were either left empty (E), or filled with solutions of LF, DP, CG, or PBS. Nerve function was assessed preoperatively and then postoperatively, every 10 days for 90 days using sciatic functional indexes (SFI). On postoperative day 90, the sciatic nerves were harvested for histologic analysis and the posterior compartment muscles of each animal were harvested and weighed. Molecular analysis for two proteins associated with neural regeneration was performed on the nerve segments. Results: All five animal groups demonstrated equivalent functional recovery. Comparison of the rate of recovery and mean maximal recovery between each group revealed no statistically significant differences, with P- values ranging from 0.30 to 0.95. Posterior compartment muscle masses were similar in all groups except for LF, whose animals had muscle masses 8-9% lower than CG, PBS, or E (P<0.05). Conclusion: Alteration of the regenerating neural microenvironment with exogenous matrix precursors (LF, DP), CG or PBS failed to improve sciatic functional recovery after nerve transection and silicon tubulization in this model. From this study, we conclude that LF, DP, CG, and PBS do not enhance the rate or degree of recovery of peripheral nerve function across a narrow gap when nerves are repaired by silicon tubulization.

KW - Peripheral nerve regeneration

KW - Sciatic functional indexes

KW - Silicon tubulization repair model

UR - http://www.scopus.com/inward/record.url?scp=0033118211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033118211&partnerID=8YFLogxK

U2 - 10.1016/S0385-8146(98)00067-4

DO - 10.1016/S0385-8146(98)00067-4

M3 - Article

C2 - 10214888

AN - SCOPUS:0033118211

VL - 26

SP - 117

EP - 122

JO - Auris Nasus Larynx

JF - Auris Nasus Larynx

SN - 0385-8146

IS - 2

ER -