Ganglioside–basic protein interaction: Protection of gangliosides against neuraminidase action

H. C. Yohe, Ronald I. Jacobson, Robert K Yu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The ability of acidic phospho‐ and sphingolipids to interact with basic proteins was studied by double diffusion analysis. The phospholipids, tri‐ and diphosphoinositide, and the sphingolipid, sulfatide, interacted with myelin basic protein as evidenced by precipitin line formation. Of the sialoglycosphingolipids (gangliosides) tested, only the myelin‐specific monosialoganglioside, GM4 , formed a precipitin line with myelin basic protein. In addition, myelin basic protein retarded the activity of Clostridium perfringens neuraminidase against GM4 and the disialoganglioside, GD1b. Examination of purified rat brain myelin suggested the presence of a neuraminidase activity intrinsic to myelin. This finding, in concert with ganglioside‐myelin basic protein complexes which selectively protect against neuraminidase, may provide a physiological explanation for the simplified ganglioside pattern found in myelin.

Original languageEnglish (US)
Pages (from-to)401-412
Number of pages12
JournalJournal of Neuroscience Research
Volume9
Issue number4
DOIs
StatePublished - Jan 1 1983

Fingerprint

Myelin Basic Protein
Gangliosides
Neuraminidase
Myelin Sheath
Precipitins
Sphingolipids
Sulfoglycosphingolipids
Clostridium perfringens
Proteins
Phospholipids
Brain
sialogangliosides

Keywords

  • acidic lipids
  • basic proteins
  • gangliosides
  • myelin
  • neuraminidase

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Ganglioside–basic protein interaction : Protection of gangliosides against neuraminidase action. / Yohe, H. C.; Jacobson, Ronald I.; Yu, Robert K.

In: Journal of Neuroscience Research, Vol. 9, No. 4, 01.01.1983, p. 401-412.

Research output: Contribution to journalArticle

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