Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells

Xiangwei Wang, Liqun Yang, Jeong Hyeon Choi, Eiko Kitamura, Chang Sheng Chang, Jane Ding, Eun J. Lee, Hongjuan Cui, Han Fei Ding

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Induction of differentiation is a therapeutic strategy in neuroblastoma, a common pediatric cancer of the sympathetic nervous system. The homeobox protein HOXC9 is a key regulator of neuroblastoma differentiation. To gain a molecular understanding of the function of HOXC9 in promoting differentiation of neuroblastoma cells, we conducted a genome-wide analysis of the HOXC9-induced differentiation program by microarray gene expression profiling and chromatin immunoprecipitation in combination with massively parallel sequencing (ChIP-seq). Here we describe in detail the experimental system, methods, and quality control for the generation of the microarray and ChIP-seq data associated with our recent publication [1].

Original languageEnglish (US)
Pages (from-to)50-52
Number of pages3
JournalGenomics Data
Volume2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Microarrays
Neuroblastoma
Cell Differentiation
Genes
Genome
Homeodomain Proteins
Pediatrics
Neurology
Gene expression
Chromatin
Quality control
High-Throughput Nucleotide Sequencing
Chromatin Immunoprecipitation
Sympathetic Nervous System
Gene Expression Profiling
Quality Control
Neoplasms
Therapeutics

Keywords

  • Differentiation
  • HOXC9
  • Neuroblastoma

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Genetics

Cite this

Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells. / Wang, Xiangwei; Yang, Liqun; Choi, Jeong Hyeon; Kitamura, Eiko; Chang, Chang Sheng; Ding, Jane; Lee, Eun J.; Cui, Hongjuan; Ding, Han Fei.

In: Genomics Data, Vol. 2, 01.01.2014, p. 50-52.

Research output: Contribution to journalArticle

Wang, Xiangwei ; Yang, Liqun ; Choi, Jeong Hyeon ; Kitamura, Eiko ; Chang, Chang Sheng ; Ding, Jane ; Lee, Eun J. ; Cui, Hongjuan ; Ding, Han Fei. / Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells. In: Genomics Data. 2014 ; Vol. 2. pp. 50-52.
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