Genomic profiling of myeloid sarcoma by array comparative genomic hybridization

George Deeb, Maria R. Baer, Daniel P. Gaile, Sheila N. Jani Sait, Maurice Barcos, Meir Wetzler, Jeffrey M. Conroy, Norma J. Nowak, John Kenneth Cowell, Richard T. Cheney

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Myeloid sarcoma (MS) is a tumor mass of myeloblasts or immature myeloid cells occurring in an extramedullary site. In this study, seven cases of MS [stomach (1), testis (1), skin (2), and lymph node (3)] and 3 synchronous and I follow-up bone marrow (BM) samples were studied for genomic abnormalities using array comparative genomic hybridization (array-CGH). Array-CGH construction used ∼5,400 bacterial artificial chromosome clones from the RPCI-11 library, spanning the human genome. Data were analyzed using the DNAcopy software and custom heuristics. All MS cases had genomic abnormalities detected by array-CGH. Unbalanced genomic abnormalities in five MS cases were confirmed by conventional cytogenetics (CC) and/or fluorescence in situ hybridization (FISH); these abnormalities included loss of 4q32.1-q35.2, 6q16.1-q21, and 12p12.2-p13.2 and gain of 8q21.2-q24.3, 8, 11q21-q25, 13q21.32-q34, 19, and 21. Array-CGH was also invaluable in identifying possible deletions, partner translocations, and breakpoints that were questionable by CC. The remaining two MS cases had genomic aberrations detected by array-CGH, but were not studied further by CC/FISH. Chromosome 8 was most commonly abnormal (3/7 cases). Identical genomic abnormalities were demonstrated in MS and in synchronous BM in two cases. These results demonstrate that array-CGH is a powerful tool to screen MS tissue for unbalanced genomic abnormalities, allowing identification of chromosome abnormalities when concurrent BM is nonanalyzable or nonleukemic.

Original languageEnglish (US)
Pages (from-to)373-383
Number of pages11
JournalGenes Chromosomes and Cancer
Volume44
Issue number4
DOIs
StatePublished - Dec 1 2005

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Myeloid Sarcoma
Comparative Genomic Hybridization
Cytogenetics
Bone Marrow
Fluorescence In Situ Hybridization
Bacterial Artificial Chromosomes
Granulocyte Precursor Cells
Chromosomes, Human, Pair 8
Human Genome
Myeloid Cells
Chromosome Aberrations
Libraries
Testis
Stomach
Software
Clone Cells
Lymph Nodes
Skin

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Deeb, G., Baer, M. R., Gaile, D. P., Jani Sait, S. N., Barcos, M., Wetzler, M., ... Cheney, R. T. (2005). Genomic profiling of myeloid sarcoma by array comparative genomic hybridization. Genes Chromosomes and Cancer, 44(4), 373-383. https://doi.org/10.1002/gcc.20239

Genomic profiling of myeloid sarcoma by array comparative genomic hybridization. / Deeb, George; Baer, Maria R.; Gaile, Daniel P.; Jani Sait, Sheila N.; Barcos, Maurice; Wetzler, Meir; Conroy, Jeffrey M.; Nowak, Norma J.; Cowell, John Kenneth; Cheney, Richard T.

In: Genes Chromosomes and Cancer, Vol. 44, No. 4, 01.12.2005, p. 373-383.

Research output: Contribution to journalArticle

Deeb, G, Baer, MR, Gaile, DP, Jani Sait, SN, Barcos, M, Wetzler, M, Conroy, JM, Nowak, NJ, Cowell, JK & Cheney, RT 2005, 'Genomic profiling of myeloid sarcoma by array comparative genomic hybridization', Genes Chromosomes and Cancer, vol. 44, no. 4, pp. 373-383. https://doi.org/10.1002/gcc.20239
Deeb G, Baer MR, Gaile DP, Jani Sait SN, Barcos M, Wetzler M et al. Genomic profiling of myeloid sarcoma by array comparative genomic hybridization. Genes Chromosomes and Cancer. 2005 Dec 1;44(4):373-383. https://doi.org/10.1002/gcc.20239
Deeb, George ; Baer, Maria R. ; Gaile, Daniel P. ; Jani Sait, Sheila N. ; Barcos, Maurice ; Wetzler, Meir ; Conroy, Jeffrey M. ; Nowak, Norma J. ; Cowell, John Kenneth ; Cheney, Richard T. / Genomic profiling of myeloid sarcoma by array comparative genomic hybridization. In: Genes Chromosomes and Cancer. 2005 ; Vol. 44, No. 4. pp. 373-383.
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