Genotypic analysis of the protease and reverse transcriptase of HIV type 1 subtype C isolates from antiretroviral drug-naive adults in Malawi

Leslie Anne Petch Lee, I. F. Hoffman, C. S. Jere, P. N. Kazembe, F. E. Martinson, D. Chilongozi, S. A. Fiscus, M. S. Cohen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The protease (PR) and reverse transcriptase (RT) regions of HIV-1 isolates from 21 antiretroviral (ARV)-naive Malawian adults were sequenced and analyzed to determine the prevalence of drug resistance-associated mutations in this population. Phylogenetic analysis confirmed that all isolates grouped with HIV-1 subtype C, the predominant subtype in Malawi. No major mutations associated with resistance to PR inhibitors (PIs), nucleoside RT inhibitors (NRTIs), or nonnucleoside RT inhibitors (NNRTIs) were found. In contrast, accessory mutations were found in the protease region at positions 10, 20, 36, 63, 77, and 93, and in the RT region at positions 118, 211, and 214. Further studies will be needed to determine the clinical impact of these polymorphisms on viral susceptibility to existing antiretroviral drugs.

Original languageEnglish (US)
Pages (from-to)799-805
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume21
Issue number9
DOIs
StatePublished - Sep 1 2005

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Malawi
Reverse Transcriptase Inhibitors
Peptide Hydrolases
Mutation
Pharmaceutical Preparations
RNA-Directed DNA Polymerase
Protease Inhibitors
Nucleosides
Drug Resistance
HIV-1
Population
Human immunodeficiency virus 1 reverse transcriptase

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Genotypic analysis of the protease and reverse transcriptase of HIV type 1 subtype C isolates from antiretroviral drug-naive adults in Malawi. / Petch Lee, Leslie Anne; Hoffman, I. F.; Jere, C. S.; Kazembe, P. N.; Martinson, F. E.; Chilongozi, D.; Fiscus, S. A.; Cohen, M. S.

In: AIDS Research and Human Retroviruses, Vol. 21, No. 9, 01.09.2005, p. 799-805.

Research output: Contribution to journalArticle

Petch Lee, LA, Hoffman, IF, Jere, CS, Kazembe, PN, Martinson, FE, Chilongozi, D, Fiscus, SA & Cohen, MS 2005, 'Genotypic analysis of the protease and reverse transcriptase of HIV type 1 subtype C isolates from antiretroviral drug-naive adults in Malawi', AIDS Research and Human Retroviruses, vol. 21, no. 9, pp. 799-805. https://doi.org/10.1089/aid.2005.21.799
Petch Lee, Leslie Anne ; Hoffman, I. F. ; Jere, C. S. ; Kazembe, P. N. ; Martinson, F. E. ; Chilongozi, D. ; Fiscus, S. A. ; Cohen, M. S. / Genotypic analysis of the protease and reverse transcriptase of HIV type 1 subtype C isolates from antiretroviral drug-naive adults in Malawi. In: AIDS Research and Human Retroviruses. 2005 ; Vol. 21, No. 9. pp. 799-805.
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