Global analysis of small non-coding RNA populations across tissues in the malaria vector, anopheles gambiae

William Bart Bryant, Savanna Ray, Mary Katherine Mills

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Malaria is a major global health problem, where the anautogenous female mosquito Anopheles gambiae serves as a major vector. In order to combat this devastating disease, understanding mosquito physiology is paramount. Numerous studies in the vector field demonstrate that small non-coding RNAs (ncRNAs) play essential roles in numerous aspects of mosquito physiology. While our previous miRNA annotation work demonstrated expression dynamics across differing tissues, miRNAs represented less than 20% of all small ncRNAs in our small RNA-Seq libraries. To this end, we systematically classified multiple small ncRNA groups across mosquito tissues. Here we (i) determined a new enriched-midgut miRNA, (ii) updated the piRNA annotation in ovaries with a genomic map of unique-mapping piRNAs, (iii) identified pan-tissue and tissue-enriched mRNA-derived small ncRNAs, and (iv) assessed AGO1-and AGO2-loading of candidate small ncRNAs. Continued research will broaden our view of small ncRNAs and greatly aide in our understanding on how these molecules contribute to mosquito physiology.

Original languageEnglish (US)
Article number406
Pages (from-to)1-14
Number of pages14
JournalInsects
Volume11
Issue number7
DOIs
StatePublished - Jul 2020
Externally publishedYes

Keywords

  • Mosquito reproduction
  • PiRNA
  • Small RNAs
  • Small non-coding RNA

ASJC Scopus subject areas

  • Insect Science

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