Global O-GlcNAc levels modulate transcription of the adipocyte secretome during chronic insulin resistance

Edith E. Wollaston-Hayden, Ruth Babette Harris, Bingqiang Liu, Robert Bridger, Ying Xu, Lance Wells

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Increased flux through the hexosamine biosynthetic pathway and the corresponding increase in intracellular glycosylation of proteins via O-linked ß-N-acetylglucosamine (O-GlcNAc) is sufficient to induce insulin resistance (IR) in multiple systems. Previously, our group used shotgun proteomics to identify multiple rodent adipocytokines and secreted proteins whose levels are modulated upon the induction of IR by indirectly and directly modulating O-GlcNAc levels. We have validated the relative levels of several of these factors using immunoblotting. Since adipocytokines levels are regulated primarily at the level of transcription and O-GlcNAc alters the function of many transcription factors, we hypothesized that elevated O-GlcNAc levels on key transcription factors are modulating secreted protein expression. Here, we show that upon the elevation of O-GlcNAc levels and the induction of IR in mature 3T3-F442a adipocytes, the transcript levels of multiple secreted proteins reflect the modulation observed at the protein level. We validate the transcript levels in male mouse models of diabetes. Using inguinal fat pads from the severely IR db/db mouse model and the mildly IR diet-induced mouse model, we have confirmed that the secreted proteins regulated by O-GlcNAc modulation in cell culture are likewise modulated in the whole animal upon a shift to IR. By comparing the promoters of similarly regulated genes, we determine that Sp1 is a common cis-acting element. Furthermore, we show that the LPL and SPARC promoters are enriched for Sp1 and O-GlcNAc modified proteins during insulin resistance in adipocytes. Thus, the O-GlcNAc modification of proteins bound to promoters, including Sp1, is linked to adipocytokine transcription during insulin resistance.

Original languageEnglish (US)
Article number223
JournalFrontiers in Endocrinology
Volume5
Issue numberDEC
DOIs
StatePublished - 2014

Keywords

  • Adipocytokines
  • Adipose tissue
  • Insulin resistance
  • O-GlcNAc
  • Transcription

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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