Glomerular β-galactosidase expression following transduction with microsphere-adenoviral complexes

Udayan Y. Bhatt, Thomas J. Sferra, Amy Johnson, Caroline Williams, Kristen Shirey, Tammy Venema, Gerard J. Nuovo, Norris Stanley Nahman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aortic injection of adenoviral-microsphere complexes is a useful technique for in vivo gene transfer (transduction) to the glomerulus. In this approach, the appearance of the foreign transprotein in the glomerulus may result from glomerular cell gene transfer and local synthesis or hepatic cell transduction followed by synthesis, secretion, and deposition in the glomerulus. We postulated that glomerular expression of the foreign transgene was the result of glomerular cell transduction. To test this question, male SD rats underwent aortic injections with adenovirus containing the LacZ expression cassette [expressing β-galactosidase (βgal)] coupled to 16 μm diameter microspheres. After 48 hours, histologic staining confirmed glomerular expression of the βgal transprotein and reverse transcription in situ polymerase chain reaction demonstrated the presence of the βgal transgene in the glomerulus. In addition, hepatic expression of the βgal transprotein was minimal and substantially less than that observed in the glomeruli. These data support the contention that adenoviral-microsphere complexes result in glomerular cell transduction with the desired transgene, followed by local transprotein synthesis. This approach may prove useful for facilitating glomerular gene transfer in the development of gene therapy for glomerulonephritis.

Original languageEnglish (US)
JournalKidney International
Volume61
Issue numberSUPPL. 1
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Galactosidases
Microspheres
Transgenes
Genes
Injections
Glomerulonephritis
Adenoviridae
Genetic Therapy
Reverse Transcription
Hepatocytes
Staining and Labeling
Polymerase Chain Reaction
Liver

Keywords

  • Adenovectors
  • Gene therapy
  • Glomerulonephritis
  • In situ PCR
  • Transduction
  • β-galactosidase

ASJC Scopus subject areas

  • Nephrology

Cite this

Glomerular β-galactosidase expression following transduction with microsphere-adenoviral complexes. / Bhatt, Udayan Y.; Sferra, Thomas J.; Johnson, Amy; Williams, Caroline; Shirey, Kristen; Venema, Tammy; Nuovo, Gerard J.; Nahman, Norris Stanley.

In: Kidney International, Vol. 61, No. SUPPL. 1, 01.01.2002.

Research output: Contribution to journalArticle

Bhatt, Udayan Y. ; Sferra, Thomas J. ; Johnson, Amy ; Williams, Caroline ; Shirey, Kristen ; Venema, Tammy ; Nuovo, Gerard J. ; Nahman, Norris Stanley. / Glomerular β-galactosidase expression following transduction with microsphere-adenoviral complexes. In: Kidney International. 2002 ; Vol. 61, No. SUPPL. 1.
@article{10fd8a3726534a2089522ce49cb59fba,
title = "Glomerular β-galactosidase expression following transduction with microsphere-adenoviral complexes",
abstract = "The aortic injection of adenoviral-microsphere complexes is a useful technique for in vivo gene transfer (transduction) to the glomerulus. In this approach, the appearance of the foreign transprotein in the glomerulus may result from glomerular cell gene transfer and local synthesis or hepatic cell transduction followed by synthesis, secretion, and deposition in the glomerulus. We postulated that glomerular expression of the foreign transgene was the result of glomerular cell transduction. To test this question, male SD rats underwent aortic injections with adenovirus containing the LacZ expression cassette [expressing β-galactosidase (βgal)] coupled to 16 μm diameter microspheres. After 48 hours, histologic staining confirmed glomerular expression of the βgal transprotein and reverse transcription in situ polymerase chain reaction demonstrated the presence of the βgal transgene in the glomerulus. In addition, hepatic expression of the βgal transprotein was minimal and substantially less than that observed in the glomeruli. These data support the contention that adenoviral-microsphere complexes result in glomerular cell transduction with the desired transgene, followed by local transprotein synthesis. This approach may prove useful for facilitating glomerular gene transfer in the development of gene therapy for glomerulonephritis.",
keywords = "Adenovectors, Gene therapy, Glomerulonephritis, In situ PCR, Transduction, β-galactosidase",
author = "Bhatt, {Udayan Y.} and Sferra, {Thomas J.} and Amy Johnson and Caroline Williams and Kristen Shirey and Tammy Venema and Nuovo, {Gerard J.} and Nahman, {Norris Stanley}",
year = "2002",
month = "1",
day = "1",
doi = "10.1046/j.1523-1755.2002.0610s1068.x",
language = "English (US)",
volume = "61",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Glomerular β-galactosidase expression following transduction with microsphere-adenoviral complexes

AU - Bhatt, Udayan Y.

AU - Sferra, Thomas J.

AU - Johnson, Amy

AU - Williams, Caroline

AU - Shirey, Kristen

AU - Venema, Tammy

AU - Nuovo, Gerard J.

AU - Nahman, Norris Stanley

PY - 2002/1/1

Y1 - 2002/1/1

N2 - The aortic injection of adenoviral-microsphere complexes is a useful technique for in vivo gene transfer (transduction) to the glomerulus. In this approach, the appearance of the foreign transprotein in the glomerulus may result from glomerular cell gene transfer and local synthesis or hepatic cell transduction followed by synthesis, secretion, and deposition in the glomerulus. We postulated that glomerular expression of the foreign transgene was the result of glomerular cell transduction. To test this question, male SD rats underwent aortic injections with adenovirus containing the LacZ expression cassette [expressing β-galactosidase (βgal)] coupled to 16 μm diameter microspheres. After 48 hours, histologic staining confirmed glomerular expression of the βgal transprotein and reverse transcription in situ polymerase chain reaction demonstrated the presence of the βgal transgene in the glomerulus. In addition, hepatic expression of the βgal transprotein was minimal and substantially less than that observed in the glomeruli. These data support the contention that adenoviral-microsphere complexes result in glomerular cell transduction with the desired transgene, followed by local transprotein synthesis. This approach may prove useful for facilitating glomerular gene transfer in the development of gene therapy for glomerulonephritis.

AB - The aortic injection of adenoviral-microsphere complexes is a useful technique for in vivo gene transfer (transduction) to the glomerulus. In this approach, the appearance of the foreign transprotein in the glomerulus may result from glomerular cell gene transfer and local synthesis or hepatic cell transduction followed by synthesis, secretion, and deposition in the glomerulus. We postulated that glomerular expression of the foreign transgene was the result of glomerular cell transduction. To test this question, male SD rats underwent aortic injections with adenovirus containing the LacZ expression cassette [expressing β-galactosidase (βgal)] coupled to 16 μm diameter microspheres. After 48 hours, histologic staining confirmed glomerular expression of the βgal transprotein and reverse transcription in situ polymerase chain reaction demonstrated the presence of the βgal transgene in the glomerulus. In addition, hepatic expression of the βgal transprotein was minimal and substantially less than that observed in the glomeruli. These data support the contention that adenoviral-microsphere complexes result in glomerular cell transduction with the desired transgene, followed by local transprotein synthesis. This approach may prove useful for facilitating glomerular gene transfer in the development of gene therapy for glomerulonephritis.

KW - Adenovectors

KW - Gene therapy

KW - Glomerulonephritis

KW - In situ PCR

KW - Transduction

KW - β-galactosidase

UR - http://www.scopus.com/inward/record.url?scp=0036175763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036175763&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.2002.0610s1068.x

DO - 10.1046/j.1523-1755.2002.0610s1068.x

M3 - Article

C2 - 11841616

AN - SCOPUS:0036175763

VL - 61

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - SUPPL. 1

ER -