Glycolipid Changes in Murine Myelogenous Leukemias

Neutral Glycolipids as Markers for Specific Populations of Leukemias

Toshio Ariga, Kazuko Yoshida, Kumie Nemoto, Masatoshi Seki, Nobuyuki Miyatani, Robert K Yu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have studied the glycolipid composition of six different murine myelogenous leukemias as well as that of T-cell leukemias and normal spleen cells. Neutral and acidic lipid fractions were isolated by column chromatography on DEAE-Sephadex and analyzed by high-performance thin-layer chromatography (HPTLC) and an HPTLC overlay method. Murine myelogenous leukemias were found to contain globo- and ganglio-series neutral glycolipids, e.g., glucosylceramide (Glc-cer), lactosylceramide (Lac-cer), globotriaosylceramide (Gb3), globoside (Gb4), Forssman glycolipid (Gb5), and asialo-GM1 (GA1). Monoblastic leukemia cells contained increased proportions of Gb3, Gb4, Gb5, and GA1. Monocytic and myelomonocytic leukemia cells contained increased proportions of Glc-cer and Lac-cer. Especially, Glc-cer accounted for approximately 60% of the total neutral glycolipids in monocytic leukemia cells. Gb3 was the major neutral glycolipid in reticulum cell neoplasm type A, and it accounted for approximately 75% of the neutral glycolipids. GA1 was the major neutral glycolipid in myeloblastic and granulocytic leukemia cells as well as T-cell leukemias. Especially, granulocytic leukemia cells contained predominantly GA1, and it accounted for approximately 80% of the total neutral glycolipids. The pattern of gangliosides in myelogenous leukemias was more complex when compared with that of the neutral glycolipids; murine myelogenous leukemias contained at least 13 gangliosides, including such major gangliosides as GM1, GM1b containing N-acetyl neuraminic acid and N-glycolyl neuraminic acid, and GalNAc-GM1b. Alterations of glycolipid composition in murine myeloid leukemias may be associated with cellular differentiation and maturation, and therefore these characteristic glycolipid species may be regarded as markers for specific populations of leukemia cells.

Original languageEnglish (US)
Pages (from-to)7953-7961
Number of pages9
JournalBiochemistry
Volume30
Issue number32
DOIs
StatePublished - Aug 1 1991
Externally publishedYes

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Myeloid Leukemia
Glycolipids
Leukemia
Population
Glucosylceramides
Gangliosides
Neuraminic Acids
T-Cell Leukemia
Thin layer chromatography
T-cells
Thin Layer Chromatography
G(M1) Ganglioside
DEAE-Dextran
Reticulum
Column chromatography
Chemical analysis
Chromatography
Spleen
Lipids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glycolipid Changes in Murine Myelogenous Leukemias : Neutral Glycolipids as Markers for Specific Populations of Leukemias. / Ariga, Toshio; Yoshida, Kazuko; Nemoto, Kumie; Seki, Masatoshi; Miyatani, Nobuyuki; Yu, Robert K.

In: Biochemistry, Vol. 30, No. 32, 01.08.1991, p. 7953-7961.

Research output: Contribution to journalArticle

Ariga, Toshio ; Yoshida, Kazuko ; Nemoto, Kumie ; Seki, Masatoshi ; Miyatani, Nobuyuki ; Yu, Robert K. / Glycolipid Changes in Murine Myelogenous Leukemias : Neutral Glycolipids as Markers for Specific Populations of Leukemias. In: Biochemistry. 1991 ; Vol. 30, No. 32. pp. 7953-7961.
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