Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

Dong Zhang; Mohamed Al-Hendy; Gloria Richard-Davis; Valerie Montgomery-Rice; Chakradhari Sharan; Veera Rajaratnam; Anjali Khurana; Ayman Al-Hendy

doi:10.1016/j.ajog.2009.10.885

Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

Dong Zhang, Mohamed Al-Hendy, Gloria Richard-Davis, Valerie Montgomery-Rice, Chakradhari Sharan, Veera Rajaratnam, Anjali Khurana, Ayman Al-Hendy

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

Original language	English (US)
Pages (from-to)	289.e1-289.e9
Journal	American journal of obstetrics and gynecology
Volume	202
Issue number	3
DOIs	https://doi.org/10.1016/j.ajog.2009.10.885
State	Published - Mar 2010
Externally published	Yes

Keywords

apoptosis
epigallocatechin gallate (EGCG)
green tea extract
inhibitory effect
uterine leiomyoma

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/j.ajog.2009.10.885

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@article{9f56a46343064393b16b2fa3c55f75a1,

title = "Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice",

abstract = "Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.",

keywords = "apoptosis, epigallocatechin gallate (EGCG), green tea extract, inhibitory effect, uterine leiomyoma",

author = "Dong Zhang and Mohamed Al-Hendy and Gloria Richard-Davis and Valerie Montgomery-Rice and Chakradhari Sharan and Veera Rajaratnam and Anjali Khurana and Ayman Al-Hendy",

note = "Funding Information: This study was supported by Research Center for Minority Institutes (RCMI) Grant G12 RR03032 and National Institutes of Health/National Institute of Child Health and Human Development 1 R01 HD046228 (A.A.). ",

year = "2010",

month = mar,

doi = "10.1016/j.ajog.2009.10.885",

language = "English (US)",

volume = "202",

pages = "289.e1--289.e9",

journal = "American journal of obstetrics and gynecology",

issn = "0002-9378",

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number = "3",

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T1 - Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

AU - Zhang, Dong

AU - Al-Hendy, Mohamed

AU - Richard-Davis, Gloria

AU - Montgomery-Rice, Valerie

AU - Sharan, Chakradhari

AU - Rajaratnam, Veera

AU - Khurana, Anjali

AU - Al-Hendy, Ayman

N1 - Funding Information: This study was supported by Research Center for Minority Institutes (RCMI) Grant G12 RR03032 and National Institutes of Health/National Institute of Child Health and Human Development 1 R01 HD046228 (A.A.).

PY - 2010/3

Y1 - 2010/3

N2 - Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

AB - Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

KW - apoptosis

KW - epigallocatechin gallate (EGCG)

KW - green tea extract

KW - inhibitory effect

KW - uterine leiomyoma

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ER -

Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

Abstract

Keywords

ASJC Scopus subject areas

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