Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

Dong Zhang, Mohamed Al-Hendy, Gloria Richard-Davis, Valerie Montgomery-Rice, Chakradhari Sharan, Veera Rajaratnam, Anjali Khurana, Ayman Al-Hendy

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)289.e1-289.e9
JournalAmerican Journal of Obstetrics and Gynecology
Volume202
Issue number3
DOIs
StatePublished - Jan 1 2010

Fingerprint

Leiomyoma
Tea
Nude Mice
Cyclin-Dependent Kinase 4
Nuclear Antigens
Cell Proliferation
Tumor Burden
Proteins
epigallocatechin gallate
In Vitro Techniques
Drinking Water
Apoptosis

Keywords

  • apoptosis
  • epigallocatechin gallate (EGCG)
  • green tea extract
  • inhibitory effect
  • uterine leiomyoma

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Zhang, D., Al-Hendy, M., Richard-Davis, G., Montgomery-Rice, V., Sharan, C., Rajaratnam, V., ... Al-Hendy, A. (2010). Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. American Journal of Obstetrics and Gynecology, 202(3), 289.e1-289.e9. https://doi.org/10.1016/j.ajog.2009.10.885

Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. / Zhang, Dong; Al-Hendy, Mohamed; Richard-Davis, Gloria; Montgomery-Rice, Valerie; Sharan, Chakradhari; Rajaratnam, Veera; Khurana, Anjali; Al-Hendy, Ayman.

In: American Journal of Obstetrics and Gynecology, Vol. 202, No. 3, 01.01.2010, p. 289.e1-289.e9.

Research output: Contribution to journalArticle

Zhang, D, Al-Hendy, M, Richard-Davis, G, Montgomery-Rice, V, Sharan, C, Rajaratnam, V, Khurana, A & Al-Hendy, A 2010, 'Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice', American Journal of Obstetrics and Gynecology, vol. 202, no. 3, pp. 289.e1-289.e9. https://doi.org/10.1016/j.ajog.2009.10.885
Zhang D, Al-Hendy M, Richard-Davis G, Montgomery-Rice V, Sharan C, Rajaratnam V et al. Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. American Journal of Obstetrics and Gynecology. 2010 Jan 1;202(3):289.e1-289.e9. https://doi.org/10.1016/j.ajog.2009.10.885
Zhang, Dong ; Al-Hendy, Mohamed ; Richard-Davis, Gloria ; Montgomery-Rice, Valerie ; Sharan, Chakradhari ; Rajaratnam, Veera ; Khurana, Anjali ; Al-Hendy, Ayman. / Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. In: American Journal of Obstetrics and Gynecology. 2010 ; Vol. 202, No. 3. pp. 289.e1-289.e9.
@article{9f56a46343064393b16b2fa3c55f75a1,
title = "Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice",
abstract = "Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.",
keywords = "apoptosis, epigallocatechin gallate (EGCG), green tea extract, inhibitory effect, uterine leiomyoma",
author = "Dong Zhang and Mohamed Al-Hendy and Gloria Richard-Davis and Valerie Montgomery-Rice and Chakradhari Sharan and Veera Rajaratnam and Anjali Khurana and Ayman Al-Hendy",
year = "2010",
month = "1",
day = "1",
doi = "10.1016/j.ajog.2009.10.885",
language = "English (US)",
volume = "202",
pages = "289.e1--289.e9",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

AU - Zhang, Dong

AU - Al-Hendy, Mohamed

AU - Richard-Davis, Gloria

AU - Montgomery-Rice, Valerie

AU - Sharan, Chakradhari

AU - Rajaratnam, Veera

AU - Khurana, Anjali

AU - Al-Hendy, Ayman

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

AB - Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

KW - apoptosis

KW - epigallocatechin gallate (EGCG)

KW - green tea extract

KW - inhibitory effect

KW - uterine leiomyoma

UR - http://www.scopus.com/inward/record.url?scp=77649190601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649190601&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2009.10.885

DO - 10.1016/j.ajog.2009.10.885

M3 - Article

VL - 202

SP - 289.e1-289.e9

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 3

ER -