Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

Dong Zhang, Mohamed Al-Hendy, Gloria Richard-Davis, Valerie Montgomery-Rice, Chakradhari Sharan, Veera Rajaratnam, Anjali Khurana, Ayman Al-Hendy

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Objective: The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model. Study Design: ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment. Results: Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05). Conclusion: EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)289.e1-289.e9
JournalAmerican Journal of Obstetrics and Gynecology
Volume202
Issue number3
DOIs
StatePublished - Jan 1 2010

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Keywords

  • apoptosis
  • epigallocatechin gallate (EGCG)
  • green tea extract
  • inhibitory effect
  • uterine leiomyoma

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Zhang, D., Al-Hendy, M., Richard-Davis, G., Montgomery-Rice, V., Sharan, C., Rajaratnam, V., Khurana, A., & Al-Hendy, A. (2010). Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. American Journal of Obstetrics and Gynecology, 202(3), 289.e1-289.e9. https://doi.org/10.1016/j.ajog.2009.10.885