GD3 ganglioside is a glycolipid characteristic of immature neuroectodermal cells

James E. Goldman, Michio Hirano, Robert K Yu, Thomas N. Seyfried

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Biochemical studies have indicated that the disialoganglioside, GD3, is a major glycolipid component of the immature vertebrate CNS, but a minor element within the mature CNS. We have investigated its cellular localization in rat CNS by immunofluorescence using a mouse monoclonal antibody that recognizes GD3. In tissue sections of postnatal CNS, the antibody bound to cells in several areas known to contain immature neuroectodermal populations: the subventricular zone beneath the lateral ventricle, the external germinative layer of the cerebellar cortex, and the dentate gyrus of the hippocampus. GD3-containing cells were also found in developing white matter of the forebrain and cerebellar folia. Using a double-label immunofluorescence method, we found that the GD3-positive white matter cells did not express the astrocytic marker, glial fibrillary acidic protein. In adult rat CNS, we could not detect antibody binding to neurons or glia. Scattered GD3-positive cells were apparent in the adult subventricular zone. Our results indicate that GD3 ganglioside is a membrane component characteristically expressed in the rat CNS by neuroectodermal stem cells, both neuronal and glial precursors.

Original languageEnglish (US)
Pages (from-to)179-192
Number of pages14
JournalJournal of Neuroimmunology
Volume7
Issue numberC
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Glycolipids
Lateral Ventricles
Neuroglia
Fluorescent Antibody Technique
Parahippocampal Gyrus
Cerebellar Cortex
Antibodies
Glial Fibrillary Acidic Protein
Dentate Gyrus
Prosencephalon
Vertebrates
Stem Cells
Monoclonal Antibodies
Neurons
Membranes
GD3 ganglioside
Population
White Matter

Keywords

  • Central nervous system
  • Disialoganglioside
  • Neuroectodermal cells
  • Rat
  • glycolipid

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

GD3 ganglioside is a glycolipid characteristic of immature neuroectodermal cells. / Goldman, James E.; Hirano, Michio; Yu, Robert K; Seyfried, Thomas N.

In: Journal of Neuroimmunology, Vol. 7, No. C, 01.01.1984, p. 179-192.

Research output: Contribution to journalArticle

Goldman, James E. ; Hirano, Michio ; Yu, Robert K ; Seyfried, Thomas N. / GD3 ganglioside is a glycolipid characteristic of immature neuroectodermal cells. In: Journal of Neuroimmunology. 1984 ; Vol. 7, No. C. pp. 179-192.
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