GD3 ganglioside is a glycolipid characteristic of immature neuroectodermal cells

James E. Goldman, Michio Hirano, Robert K. Yu, Thomas N. Seyfried

Research output: Contribution to journalArticle

159 Scopus citations

Abstract

Biochemical studies have indicated that the disialoganglioside, GD3, is a major glycolipid component of the immature vertebrate CNS, but a minor element within the mature CNS. We have investigated its cellular localization in rat CNS by immunofluorescence using a mouse monoclonal antibody that recognizes GD3. In tissue sections of postnatal CNS, the antibody bound to cells in several areas known to contain immature neuroectodermal populations: the subventricular zone beneath the lateral ventricle, the external germinative layer of the cerebellar cortex, and the dentate gyrus of the hippocampus. GD3-containing cells were also found in developing white matter of the forebrain and cerebellar folia. Using a double-label immunofluorescence method, we found that the GD3-positive white matter cells did not express the astrocytic marker, glial fibrillary acidic protein. In adult rat CNS, we could not detect antibody binding to neurons or glia. Scattered GD3-positive cells were apparent in the adult subventricular zone. Our results indicate that GD3 ganglioside is a membrane component characteristically expressed in the rat CNS by neuroectodermal stem cells, both neuronal and glial precursors.

Original languageEnglish (US)
Pages (from-to)179-192
Number of pages14
JournalJournal of Neuroimmunology
Volume7
Issue numberC
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Keywords

  • Central nervous system
  • Disialoganglioside
  • Neuroectodermal cells
  • Rat
  • glycolipid

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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