Abstract
Biochemical studies have indicated that the disialoganglioside, GD3, is a major glycolipid component of the immature vertebrate CNS, but a minor element within the mature CNS. We have investigated its cellular localization in rat CNS by immunofluorescence using a mouse monoclonal antibody that recognizes GD3. In tissue sections of postnatal CNS, the antibody bound to cells in several areas known to contain immature neuroectodermal populations: the subventricular zone beneath the lateral ventricle, the external germinative layer of the cerebellar cortex, and the dentate gyrus of the hippocampus. GD3-containing cells were also found in developing white matter of the forebrain and cerebellar folia. Using a double-label immunofluorescence method, we found that the GD3-positive white matter cells did not express the astrocytic marker, glial fibrillary acidic protein. In adult rat CNS, we could not detect antibody binding to neurons or glia. Scattered GD3-positive cells were apparent in the adult subventricular zone. Our results indicate that GD3 ganglioside is a membrane component characteristically expressed in the rat CNS by neuroectodermal stem cells, both neuronal and glial precursors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 179-192 |
| Number of pages | 14 |
| Journal | Journal of Neuroimmunology |
| Volume | 7 |
| Issue number | C |
| DOIs | |
| State | Published - Jan 1 1984 |
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Keywords
- Central nervous system
- Disialoganglioside
- Neuroectodermal cells
- Rat
- glycolipid
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology
Cite this
GD3 ganglioside is a glycolipid characteristic of immature neuroectodermal cells. / Goldman, James E.; Hirano, Michio; Yu, Robert K.; Seyfried, Thomas N.
In: Journal of Neuroimmunology, Vol. 7, No. C, 01.01.1984, p. 179-192.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - GD3 ganglioside is a glycolipid characteristic of immature neuroectodermal cells
AU - Goldman, James E.
AU - Hirano, Michio
AU - Yu, Robert K.
AU - Seyfried, Thomas N.
PY - 1984/1/1
Y1 - 1984/1/1
N2 - Biochemical studies have indicated that the disialoganglioside, GD3, is a major glycolipid component of the immature vertebrate CNS, but a minor element within the mature CNS. We have investigated its cellular localization in rat CNS by immunofluorescence using a mouse monoclonal antibody that recognizes GD3. In tissue sections of postnatal CNS, the antibody bound to cells in several areas known to contain immature neuroectodermal populations: the subventricular zone beneath the lateral ventricle, the external germinative layer of the cerebellar cortex, and the dentate gyrus of the hippocampus. GD3-containing cells were also found in developing white matter of the forebrain and cerebellar folia. Using a double-label immunofluorescence method, we found that the GD3-positive white matter cells did not express the astrocytic marker, glial fibrillary acidic protein. In adult rat CNS, we could not detect antibody binding to neurons or glia. Scattered GD3-positive cells were apparent in the adult subventricular zone. Our results indicate that GD3 ganglioside is a membrane component characteristically expressed in the rat CNS by neuroectodermal stem cells, both neuronal and glial precursors.
AB - Biochemical studies have indicated that the disialoganglioside, GD3, is a major glycolipid component of the immature vertebrate CNS, but a minor element within the mature CNS. We have investigated its cellular localization in rat CNS by immunofluorescence using a mouse monoclonal antibody that recognizes GD3. In tissue sections of postnatal CNS, the antibody bound to cells in several areas known to contain immature neuroectodermal populations: the subventricular zone beneath the lateral ventricle, the external germinative layer of the cerebellar cortex, and the dentate gyrus of the hippocampus. GD3-containing cells were also found in developing white matter of the forebrain and cerebellar folia. Using a double-label immunofluorescence method, we found that the GD3-positive white matter cells did not express the astrocytic marker, glial fibrillary acidic protein. In adult rat CNS, we could not detect antibody binding to neurons or glia. Scattered GD3-positive cells were apparent in the adult subventricular zone. Our results indicate that GD3 ganglioside is a membrane component characteristically expressed in the rat CNS by neuroectodermal stem cells, both neuronal and glial precursors.
KW - Central nervous system
KW - Disialoganglioside
KW - Neuroectodermal cells
KW - Rat
KW - glycolipid
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UR - http://www.scopus.com/inward/citedby.url?scp=0021661151&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(84)80017-X
DO - 10.1016/S0165-5728(84)80017-X
M3 - Article
C2 - 6392333
AN - SCOPUS:0021661151
VL - 7
SP - 179
EP - 192
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - C
ER -