Abstract
Biomineralisation is a well-regulated process mediated by extracellular matrix proteins. Biomimetic remineralisation strategies should reproduce the dimension and structural hierarchy of apatite deposits within a demineralised collagen matrix. Interfibrillar and intrafibrillar remineralisation of phosphoric acid-etched human dentine was demonstrated in this study using a Portland cement/phosphate-containing fluid system in the presence of polyacrylic acid and polyvinylphosphonic acid as respective calcium phosphate- and collagen-binding matrix protein analogues. Metastable amorphous calcium phosphate nanoprecursors were generated when polyacrylic acid was included in the phosphate-containing fluid. When both polyvinylphosphonic acid and polyacrylic acid were included, these nanoprecursors were attracted to the acid-demineralised collagen matrix and transformed into polyelectrolyte-stabilised apatite nanocrystals that assembled along the microfibrils (intrafibrillar remineralisation) and surface of the collagen fibrils (interfibrillar remineralisation). Transition from nanocrystals to larger apatite platelets probably occurred via the formation of mesocrystal intermediates. Guided tissue remineralisation is potentially useful in the remineralisation of acid-etched dentine that is incompletely infiltrated by dentine adhesives, as well as partially demineralised caries-affected dentine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1127-1137 |
| Number of pages | 11 |
| Journal | Biomaterials |
| Volume | 29 |
| Issue number | 8 |
| DOIs | |
| State | Published - Mar 1 2008 |
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Keywords
- Bioactivity
- Biomimetic material
- Biomineralisation
- Dentine
- Portland cement
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Guided tissue remineralisation of partially demineralised human dentine. / Tay, Franklin Chi Meng; Pashley, David Henry.
In: Biomaterials, Vol. 29, No. 8, 01.03.2008, p. 1127-1137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Guided tissue remineralisation of partially demineralised human dentine
AU - Tay, Franklin Chi Meng
AU - Pashley, David Henry
PY - 2008/3/1
Y1 - 2008/3/1
N2 - Biomineralisation is a well-regulated process mediated by extracellular matrix proteins. Biomimetic remineralisation strategies should reproduce the dimension and structural hierarchy of apatite deposits within a demineralised collagen matrix. Interfibrillar and intrafibrillar remineralisation of phosphoric acid-etched human dentine was demonstrated in this study using a Portland cement/phosphate-containing fluid system in the presence of polyacrylic acid and polyvinylphosphonic acid as respective calcium phosphate- and collagen-binding matrix protein analogues. Metastable amorphous calcium phosphate nanoprecursors were generated when polyacrylic acid was included in the phosphate-containing fluid. When both polyvinylphosphonic acid and polyacrylic acid were included, these nanoprecursors were attracted to the acid-demineralised collagen matrix and transformed into polyelectrolyte-stabilised apatite nanocrystals that assembled along the microfibrils (intrafibrillar remineralisation) and surface of the collagen fibrils (interfibrillar remineralisation). Transition from nanocrystals to larger apatite platelets probably occurred via the formation of mesocrystal intermediates. Guided tissue remineralisation is potentially useful in the remineralisation of acid-etched dentine that is incompletely infiltrated by dentine adhesives, as well as partially demineralised caries-affected dentine.
AB - Biomineralisation is a well-regulated process mediated by extracellular matrix proteins. Biomimetic remineralisation strategies should reproduce the dimension and structural hierarchy of apatite deposits within a demineralised collagen matrix. Interfibrillar and intrafibrillar remineralisation of phosphoric acid-etched human dentine was demonstrated in this study using a Portland cement/phosphate-containing fluid system in the presence of polyacrylic acid and polyvinylphosphonic acid as respective calcium phosphate- and collagen-binding matrix protein analogues. Metastable amorphous calcium phosphate nanoprecursors were generated when polyacrylic acid was included in the phosphate-containing fluid. When both polyvinylphosphonic acid and polyacrylic acid were included, these nanoprecursors were attracted to the acid-demineralised collagen matrix and transformed into polyelectrolyte-stabilised apatite nanocrystals that assembled along the microfibrils (intrafibrillar remineralisation) and surface of the collagen fibrils (interfibrillar remineralisation). Transition from nanocrystals to larger apatite platelets probably occurred via the formation of mesocrystal intermediates. Guided tissue remineralisation is potentially useful in the remineralisation of acid-etched dentine that is incompletely infiltrated by dentine adhesives, as well as partially demineralised caries-affected dentine.
KW - Bioactivity
KW - Biomimetic material
KW - Biomineralisation
KW - Dentine
KW - Portland cement
UR - http://www.scopus.com/inward/record.url?scp=37349095070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37349095070&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2007.11.001
DO - 10.1016/j.biomaterials.2007.11.001
M3 - Article
C2 - 18022228
AN - SCOPUS:37349095070
VL - 29
SP - 1127
EP - 1137
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 8
ER -