Guided tissue remineralisation of partially demineralised human dentine

Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

Biomineralisation is a well-regulated process mediated by extracellular matrix proteins. Biomimetic remineralisation strategies should reproduce the dimension and structural hierarchy of apatite deposits within a demineralised collagen matrix. Interfibrillar and intrafibrillar remineralisation of phosphoric acid-etched human dentine was demonstrated in this study using a Portland cement/phosphate-containing fluid system in the presence of polyacrylic acid and polyvinylphosphonic acid as respective calcium phosphate- and collagen-binding matrix protein analogues. Metastable amorphous calcium phosphate nanoprecursors were generated when polyacrylic acid was included in the phosphate-containing fluid. When both polyvinylphosphonic acid and polyacrylic acid were included, these nanoprecursors were attracted to the acid-demineralised collagen matrix and transformed into polyelectrolyte-stabilised apatite nanocrystals that assembled along the microfibrils (intrafibrillar remineralisation) and surface of the collagen fibrils (interfibrillar remineralisation). Transition from nanocrystals to larger apatite platelets probably occurred via the formation of mesocrystal intermediates. Guided tissue remineralisation is potentially useful in the remineralisation of acid-etched dentine that is incompletely infiltrated by dentine adhesives, as well as partially demineralised caries-affected dentine.

Original languageEnglish (US)
Pages (from-to)1127-1137
Number of pages11
JournalBiomaterials
Volume29
Issue number8
DOIs
StatePublished - Mar 1 2008

Fingerprint

carbopol 940
Dentin
Apatites
Collagen
Tissue
Acids
Apatite
Nanoparticles
Nanocrystals
Phosphates
Microfibrils
Biomineralization
Calcium phosphate
Biomimetics
Fluids
Extracellular Matrix Proteins
Portland cement
Platelets
Polyelectrolytes
Adhesives

Keywords

  • Bioactivity
  • Biomimetic material
  • Biomineralisation
  • Dentine
  • Portland cement

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Guided tissue remineralisation of partially demineralised human dentine. / Tay, Franklin Chi Meng; Pashley, David Henry.

In: Biomaterials, Vol. 29, No. 8, 01.03.2008, p. 1127-1137.

Research output: Contribution to journalArticle

Tay, Franklin Chi Meng ; Pashley, David Henry. / Guided tissue remineralisation of partially demineralised human dentine. In: Biomaterials. 2008 ; Vol. 29, No. 8. pp. 1127-1137.
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