Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family

J. E. Bowman, R. Bloom, S. S. Chen, B. B. Webber, J. B. Wilson, Ferdane Kutlar, Abdullah Kutlar, T. B.J. Huisman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Hb Chicago is a newly discovered hemoglobin variant which was present in a Black newborn baby and her father. The leucine residue at α136, which normally participates in the contact with the heme group, is replaced by a methionine residue. The two heterozygotes were clinically well with normal hematological data. Isolation of the αx and αA chains by reverse phase high performance liquid chromatography and hydrolysis of these chains with dilute formic acid at 110°C for 24 hours, followed by separation of the resulting peptides by reverse phase high performance liquid chromatography, greatly facilitated the final identification of the abnormality. The baby and both parents had a-Gγ-Gγ-globin gene arrangement on one chromosome (normal:-Gγ-Aγ which explains the high a Gγ values in the Hb F of these three persons.

Original languageEnglish (US)
Pages (from-to)495-505
Number of pages11
JournalHemoglobin
Volume10
Issue number5
DOIs
StatePublished - Jan 1 1986

Fingerprint

formic acid
High performance liquid chromatography
Reverse-Phase Chromatography
Chromosomes, Human, 21-22 and Y
High Pressure Liquid Chromatography
Gene Order
Globins
Heterozygote
Chromosomes
Heme
Leucine
Fathers
Methionine
Hydrolysis
Hemoglobins
Genes
Parents
Newborn Infant
Peptides
hemoglobin Chicago

ASJC Scopus subject areas

  • Hematology
  • Genetics(clinical)
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Bowman, J. E., Bloom, R., Chen, S. S., Webber, B. B., Wilson, J. B., Kutlar, F., ... Huisman, T. B. J. (1986). Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family. Hemoglobin, 10(5), 495-505. https://doi.org/10.3109/03630268609014134

Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family. / Bowman, J. E.; Bloom, R.; Chen, S. S.; Webber, B. B.; Wilson, J. B.; Kutlar, Ferdane; Kutlar, Abdullah; Huisman, T. B.J.

In: Hemoglobin, Vol. 10, No. 5, 01.01.1986, p. 495-505.

Research output: Contribution to journalArticle

Bowman, JE, Bloom, R, Chen, SS, Webber, BB, Wilson, JB, Kutlar, F, Kutlar, A & Huisman, TBJ 1986, 'Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family', Hemoglobin, vol. 10, no. 5, pp. 495-505. https://doi.org/10.3109/03630268609014134
Bowman JE, Bloom R, Chen SS, Webber BB, Wilson JB, Kutlar F et al. Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family. Hemoglobin. 1986 Jan 1;10(5):495-505. https://doi.org/10.3109/03630268609014134
Bowman, J. E. ; Bloom, R. ; Chen, S. S. ; Webber, B. B. ; Wilson, J. B. ; Kutlar, Ferdane ; Kutlar, Abdullah ; Huisman, T. B.J. / Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family. In: Hemoglobin. 1986 ; Vol. 10, No. 5. pp. 495-505.
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AU - Chen, S. S.

AU - Webber, B. B.

AU - Wilson, J. B.

AU - Kutlar, Ferdane

AU - Kutlar, Abdullah

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AB - Hb Chicago is a newly discovered hemoglobin variant which was present in a Black newborn baby and her father. The leucine residue at α136, which normally participates in the contact with the heme group, is replaced by a methionine residue. The two heterozygotes were clinically well with normal hematological data. Isolation of the αx and αA chains by reverse phase high performance liquid chromatography and hydrolysis of these chains with dilute formic acid at 110°C for 24 hours, followed by separation of the resulting peptides by reverse phase high performance liquid chromatography, greatly facilitated the final identification of the abnormality. The baby and both parents had a-Gγ-Gγ-globin gene arrangement on one chromosome (normal:-Gγ-Aγ which explains the high a Gγ values in the Hb F of these three persons.

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