Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family

J. E. Bowman, R. Bloom, S. S. Chen, B. B. Webber, J. B. Wilson, F. Kutlar, A. Kutlar, T. B.J. Huisman

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Hb Chicago is a newly discovered hemoglobin variant which was present in a Black newborn baby and her father. The leucine residue at α136, which normally participates in the contact with the heme group, is replaced by a methionine residue. The two heterozygotes were clinically well with normal hematological data. Isolation of the αx and αA chains by reverse phase high performance liquid chromatography and hydrolysis of these chains with dilute formic acid at 110°C for 24 hours, followed by separation of the resulting peptides by reverse phase high performance liquid chromatography, greatly facilitated the final identification of the abnormality. The baby and both parents had a-Gγ-Gγ-globin gene arrangement on one chromosome (normal:-Gγ-Aγ which explains the high a Gγ values in the Hb F of these three persons.

Original languageEnglish (US)
Pages (from-to)495-505
Number of pages11
Issue number5
StatePublished - 1986

ASJC Scopus subject areas

  • Hematology
  • Clinical Biochemistry
  • Genetics(clinical)
  • Biochemistry, medical


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