Abstract
Hb Chicago is a newly discovered hemoglobin variant which was present in a Black newborn baby and her father. The leucine residue at α136, which normally participates in the contact with the heme group, is replaced by a methionine residue. The two heterozygotes were clinically well with normal hematological data. Isolation of the αx and αA chains by reverse phase high performance liquid chromatography and hydrolysis of these chains with dilute formic acid at 110°C for 24 hours, followed by separation of the resulting peptides by reverse phase high performance liquid chromatography, greatly facilitated the final identification of the abnormality. The baby and both parents had a-Gγ-Gγ-globin gene arrangement on one chromosome (normal:-Gγ-Aγ which explains the high a Gγ values in the Hb F of these three persons.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 495-505 |
| Number of pages | 11 |
| Journal | Hemoglobin |
| Volume | 10 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 1986 |
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ASJC Scopus subject areas
- Hematology
- Genetics(clinical)
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family. / Bowman, J. E.; Bloom, R.; Chen, S. S.; Webber, B. B.; Wilson, J. B.; Kutlar, Ferdane; Kutlar, Abdullah; Huisman, T. B.J.
In: Hemoglobin, Vol. 10, No. 5, 01.01.1986, p. 495-505.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hb chicago or α2136(h19)LEU+METβ2 and a-gγ-gGMglobin GWE arrangewznt in a black family
AU - Bowman, J. E.
AU - Bloom, R.
AU - Chen, S. S.
AU - Webber, B. B.
AU - Wilson, J. B.
AU - Kutlar, Ferdane
AU - Kutlar, Abdullah
AU - Huisman, T. B.J.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Hb Chicago is a newly discovered hemoglobin variant which was present in a Black newborn baby and her father. The leucine residue at α136, which normally participates in the contact with the heme group, is replaced by a methionine residue. The two heterozygotes were clinically well with normal hematological data. Isolation of the αx and αA chains by reverse phase high performance liquid chromatography and hydrolysis of these chains with dilute formic acid at 110°C for 24 hours, followed by separation of the resulting peptides by reverse phase high performance liquid chromatography, greatly facilitated the final identification of the abnormality. The baby and both parents had a-Gγ-Gγ-globin gene arrangement on one chromosome (normal:-Gγ-Aγ which explains the high a Gγ values in the Hb F of these three persons.
AB - Hb Chicago is a newly discovered hemoglobin variant which was present in a Black newborn baby and her father. The leucine residue at α136, which normally participates in the contact with the heme group, is replaced by a methionine residue. The two heterozygotes were clinically well with normal hematological data. Isolation of the αx and αA chains by reverse phase high performance liquid chromatography and hydrolysis of these chains with dilute formic acid at 110°C for 24 hours, followed by separation of the resulting peptides by reverse phase high performance liquid chromatography, greatly facilitated the final identification of the abnormality. The baby and both parents had a-Gγ-Gγ-globin gene arrangement on one chromosome (normal:-Gγ-Aγ which explains the high a Gγ values in the Hb F of these three persons.
UR - http://www.scopus.com/inward/record.url?scp=0023035772&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023035772&partnerID=8YFLogxK
U2 - 10.3109/03630268609014134
DO - 10.3109/03630268609014134
M3 - Article
VL - 10
SP - 495
EP - 505
JO - Hemoglobin
JF - Hemoglobin
SN - 0363-0269
IS - 5
ER -