Helicobacter priori does not require Lewis X or Lewis Y expression to colonize C3H/HeJ mice

Tohru Takata, Emad El-Omar, Margarita Camorlinga, Stuart A. Thompson, Yutaka Minohara, Peter B. Ernst, Martin J. Blaser

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Helicobacter pylori strains frequently express Lewis X (Lex) and/or Ley on their cell surfaces as constituents of the O antigens of their lipopolysaccharide molecules. To assess the effect of Lex and Ley expression on the ability of H. pylori to colonize the mouse stomach and to adhere to epithelial cells, isogenic mutants were created in which fucT1 alone or fucT1 and fucT2, which encode the fucosyl transferases necessary for Lex and Ley expression, were deleted. C3H/HeJ mice were experimentally challenged with either wild-type 26695 H. pylori or its isogenic mutants. All strains, whether passaged in the laboratory or recovered after mouse passage, colonized the mice well and without consistent differences. During colonization by the mutants, there was no reversion to wild type. Similarly, adherence to AGS and KatoIII cells was unaffected by the mutations. Together, these findings indicate that Le expression is not necessary for mouse gastric colonization or for H. pylori adherence to epithelial cells.

Original languageEnglish (US)
Pages (from-to)3073-3079
Number of pages7
JournalInfection and Immunity
Volume70
Issue number6
DOIs
StatePublished - Jan 1 2002

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Helicobacter
Inbred C3H Mouse
Helicobacter pylori
Stomach
Epithelial Cells
O Antigens
Transferases
Lipopolysaccharides
Mutation

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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Helicobacter priori does not require Lewis X or Lewis Y expression to colonize C3H/HeJ mice. / Takata, Tohru; El-Omar, Emad; Camorlinga, Margarita; Thompson, Stuart A.; Minohara, Yutaka; Ernst, Peter B.; Blaser, Martin J.

In: Infection and Immunity, Vol. 70, No. 6, 01.01.2002, p. 3073-3079.

Research output: Contribution to journalArticle

Takata, T, El-Omar, E, Camorlinga, M, Thompson, SA, Minohara, Y, Ernst, PB & Blaser, MJ 2002, 'Helicobacter priori does not require Lewis X or Lewis Y expression to colonize C3H/HeJ mice', Infection and Immunity, vol. 70, no. 6, pp. 3073-3079. https://doi.org/10.1128/IAI.70.6.3073-3079.2002
Takata, Tohru ; El-Omar, Emad ; Camorlinga, Margarita ; Thompson, Stuart A. ; Minohara, Yutaka ; Ernst, Peter B. ; Blaser, Martin J. / Helicobacter priori does not require Lewis X or Lewis Y expression to colonize C3H/HeJ mice. In: Infection and Immunity. 2002 ; Vol. 70, No. 6. pp. 3073-3079.
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