Hexavalent uranium supports growth of Anaeromyxobacter dehalogenans and Geobacter spp. with lower than predicted biomass yields

Robert A. Sanford, Qingzhong Wu, Youlboong Sung, Sara H. Thomas, Benjamin K. Amos, Emily Katherine Prince, Frank E. Löffler

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The stimulation of bacteria capable of reducing soluble U(VI) to sparingly soluble U(IV) is a promising approach for containing U(VI) plumes. Anaeromyxobacter dehalogenans is capable of mediating this activity; however, its ability to couple U(VI) reduction to growth has not been established. Monitoring the increase in 16S rRNA gene copy numbers using quantitative real-time PCR (qPCR) in cultures provided with U(VI) as an electron acceptor demonstrated growth, and 7.7-8.6 × 106 cells were produced per μmole of U(VI) reduced. This biomass yield was lower than predicted based on the theoretical free energy changes associated with U(VI)-to-U(IV) reduction. Lower than predicted growth yields with U(VI) as electron acceptor were also determined in cultures of Geobacter lovleyi and Geobacter sulfurreducens suggesting that U(VI) reduction is inefficient or imposes an additional cost to growing cells. These findings have implications for U(VI) bioremediation because Anaeromyxobacter spp. and Geobacter spp. contribute to radionuclide immobilization in contaminated subsurface environments.

Original languageEnglish (US)
Pages (from-to)2885-2893
Number of pages9
JournalEnvironmental Microbiology
Volume9
Issue number11
DOIs
StatePublished - Nov 1 2007

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Anaeromyxobacter dehalogenans
Geobacter
Uranium
uranium
Biomass
Anaeromyxobacter
Geobacter sulfurreducens
electrons
gene dosage
biomass
bioremediation
radionuclides
Growth
Electrons
electron
quantitative polymerase chain reaction
Environmental Biodegradation
Gene Dosage
ribosomal RNA
cells

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Hexavalent uranium supports growth of Anaeromyxobacter dehalogenans and Geobacter spp. with lower than predicted biomass yields. / Sanford, Robert A.; Wu, Qingzhong; Sung, Youlboong; Thomas, Sara H.; Amos, Benjamin K.; Prince, Emily Katherine; Löffler, Frank E.

In: Environmental Microbiology, Vol. 9, No. 11, 01.11.2007, p. 2885-2893.

Research output: Contribution to journalArticle

Sanford, Robert A. ; Wu, Qingzhong ; Sung, Youlboong ; Thomas, Sara H. ; Amos, Benjamin K. ; Prince, Emily Katherine ; Löffler, Frank E. / Hexavalent uranium supports growth of Anaeromyxobacter dehalogenans and Geobacter spp. with lower than predicted biomass yields. In: Environmental Microbiology. 2007 ; Vol. 9, No. 11. pp. 2885-2893.
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