Hexosaminidase: A marker for healing after ischemic gut injury

Thom E. Lobe; Eric E. Dobkin; David K. Rassin; William K. Gourley; Keith T. Oldham; Jatinder Bhatia

doi:10.1016/0022-4804(86)90200-3

Hexosaminidase: A marker for healing after ischemic gut injury

Thom E. Lobe, Eric E. Dobkin, David K. Rassin, William K. Gourley, Keith T. Oldham, Jatinder Bhatia

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Serum hexosaminidase activity (HEX) is elevated with ischemic gut injury. To determine if subsequent decreases in HEX correlate with gut healing, 97 weanling rats were subjected to laparotomy at which alternate vascular bundles were ligated along the base of the entire anterior mesenteric artery arcade. Fifteen rats served as preoperative controls. After recovery, rats were allowed ad lib food and water. Groups were then killed at intervals, blood was drawn for HEX determination, and samples of small bowel were taken for histological evaluation. Microscopically, focal ischemic necrosis began at 6 hr. Between 12 and 48 hr, cellular changes were consistent with progressive ischemic injury. Evidence of healing was apparent beginning at 5 days and these histological changes correlated with changes in HEX. Thus HEX proves useful as a marker for gut healing in this model.

Original language	English (US)
Pages (from-to)	362-367
Number of pages	6
Journal	Journal of Surgical Research
Volume	40
Issue number	4
DOIs	https://doi.org/10.1016/0022-4804(86)90200-3
State	Published - Apr 1986
Externally published	Yes

ASJC Scopus subject areas

Surgery

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title = "Hexosaminidase: A marker for healing after ischemic gut injury",

abstract = "Serum hexosaminidase activity (HEX) is elevated with ischemic gut injury. To determine if subsequent decreases in HEX correlate with gut healing, 97 weanling rats were subjected to laparotomy at which alternate vascular bundles were ligated along the base of the entire anterior mesenteric artery arcade. Fifteen rats served as preoperative controls. After recovery, rats were allowed ad lib food and water. Groups were then killed at intervals, blood was drawn for HEX determination, and samples of small bowel were taken for histological evaluation. Microscopically, focal ischemic necrosis began at 6 hr. Between 12 and 48 hr, cellular changes were consistent with progressive ischemic injury. Evidence of healing was apparent beginning at 5 days and these histological changes correlated with changes in HEX. Thus HEX proves useful as a marker for gut healing in this model.",

author = "Lobe, {Thom E.} and Dobkin, {Eric E.} and Rassin, {David K.} and Gourley, {William K.} and Oldham, {Keith T.} and Jatinder Bhatia",

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T1 - Hexosaminidase

T2 - A marker for healing after ischemic gut injury

AU - Lobe, Thom E.

AU - Dobkin, Eric E.

AU - Rassin, David K.

AU - Gourley, William K.

AU - Oldham, Keith T.

AU - Bhatia, Jatinder

PY - 1986/4

Y1 - 1986/4

N2 - Serum hexosaminidase activity (HEX) is elevated with ischemic gut injury. To determine if subsequent decreases in HEX correlate with gut healing, 97 weanling rats were subjected to laparotomy at which alternate vascular bundles were ligated along the base of the entire anterior mesenteric artery arcade. Fifteen rats served as preoperative controls. After recovery, rats were allowed ad lib food and water. Groups were then killed at intervals, blood was drawn for HEX determination, and samples of small bowel were taken for histological evaluation. Microscopically, focal ischemic necrosis began at 6 hr. Between 12 and 48 hr, cellular changes were consistent with progressive ischemic injury. Evidence of healing was apparent beginning at 5 days and these histological changes correlated with changes in HEX. Thus HEX proves useful as a marker for gut healing in this model.

AB - Serum hexosaminidase activity (HEX) is elevated with ischemic gut injury. To determine if subsequent decreases in HEX correlate with gut healing, 97 weanling rats were subjected to laparotomy at which alternate vascular bundles were ligated along the base of the entire anterior mesenteric artery arcade. Fifteen rats served as preoperative controls. After recovery, rats were allowed ad lib food and water. Groups were then killed at intervals, blood was drawn for HEX determination, and samples of small bowel were taken for histological evaluation. Microscopically, focal ischemic necrosis began at 6 hr. Between 12 and 48 hr, cellular changes were consistent with progressive ischemic injury. Evidence of healing was apparent beginning at 5 days and these histological changes correlated with changes in HEX. Thus HEX proves useful as a marker for gut healing in this model.

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Hexosaminidase: A marker for healing after ischemic gut injury

Abstract

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