Hippocampal brain-derived neurotrophic factor determines recruitment of anatomically connected networks after stress in diabetic mice

Marlena Wosiski-Kuhn, Mihail Bota, Christina A. Snider, Steven P. Wilson, Kannan U. Venkataraju, Pavel Osten, Alexis Michelle Stranahan

Research output: Contribution to journalArticle

Abstract

Diabetes increases adrenal steroids in humans and animal models, but potential interactions with psychological stress remain poorly understood. Diabetic rodents exhibit anxiety and reductions in hippocampal brain-derived neurotrophic factor (BDNF) expression, and these studies investigated whether loss of BDNF-driven hippocampal activity promotes anxiety and disinhibits the HPA axis. Mice with genetic obesity and diabetes (db/db) received intrahippocampal injections of lentivirus for BDNF overexpression (db/db-BDNFOE), and Wt mice received lentiviral constructs for BDNF knockdown (Wt-BDNFKD). Behavioral anxiety and glucocorticoid responses to acute restraint were compared with mice that received a fluorescent reporter (Wt-GFP, db/db-GFP). These experiments revealed that changes in hippocampal BDNF were necessary and sufficient for behavioral anxiety and HPA axis disinhibition. To examine patterns of stress-induced regional activity, we used algorithmic detection of cFos and automated segmentation of forebrain regions to generate maps of functional covariance, which were subsequently aligned with anatomical connectivity weights from the Brain Architecture Management database. db/db-GFP mice exhibited reduced activation of the hippocampal ventral subiculum (vSub) and anterior bed nucleus of stria terminalis (aBNST), and increases in the paraventricular hypothalamus (PVH), relative to Wt-GFP. BDNFKD recapitulated this pattern in Wt mice, and BDNFOE normalized activation of the vSub > aBNST > PVH pathway in db/db mice. Analysis of forebrain activation revealed largely overlapping patterns of network disruption in db/db-GFP and Wt-BDNFKD mice, implicating BDNF-driven hippocampal activity as a determinant of stress vulnerability in both the intact and diabetic brain.

Original languageEnglish (US)
Pages (from-to)900-912
Number of pages13
JournalHippocampus
Volume28
Issue number12
DOIs
StatePublished - Dec 1 2018

Fingerprint

Brain-Derived Neurotrophic Factor
Anxiety
Septal Nuclei
Prosencephalon
Hypothalamus
Hippocampus
Activation Analysis
Lentivirus
Brain
Psychological Stress
Glucocorticoids
Rodentia
Animal Models
Obesity
Steroids
Databases
Weights and Measures
Injections

Keywords

  • BDNF
  • BNST
  • HPA axis
  • anxiety
  • iDISCO

ASJC Scopus subject areas

  • Cognitive Neuroscience

Cite this

Hippocampal brain-derived neurotrophic factor determines recruitment of anatomically connected networks after stress in diabetic mice. / Wosiski-Kuhn, Marlena; Bota, Mihail; Snider, Christina A.; Wilson, Steven P.; Venkataraju, Kannan U.; Osten, Pavel; Stranahan, Alexis Michelle.

In: Hippocampus, Vol. 28, No. 12, 01.12.2018, p. 900-912.

Research output: Contribution to journalArticle

Wosiski-Kuhn, Marlena ; Bota, Mihail ; Snider, Christina A. ; Wilson, Steven P. ; Venkataraju, Kannan U. ; Osten, Pavel ; Stranahan, Alexis Michelle. / Hippocampal brain-derived neurotrophic factor determines recruitment of anatomically connected networks after stress in diabetic mice. In: Hippocampus. 2018 ; Vol. 28, No. 12. pp. 900-912.
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