TY - JOUR
T1 - Histologic Response of Human Pulp and Periapical Tissues to Tricalcium Silicate–based Materials
T2 - A Series of Successfully Treated Cases
AU - Ricucci, Domenico
AU - Grande, Nicola Maria
AU - Plotino, Gianluca
AU - Tay, Franklin R.
N1 - Publisher Copyright:
© 2019 American Association of Endodontists
PY - 2020/2
Y1 - 2020/2
N2 - Pulpal/periapical responses of human teeth that are treated successfully with tricalcium silicate–based materials are extremely difficult to obtain because of the typical unavailability of these teeth for histologic examination. The present case series reports histologic and histobacteriologic findings of 3 human teeth that had undergone pulpotomy, orthograde retreatment, and apicoectomy/root-end filling using tricalcium silicate–based endodontic materials. The teeth were extracted after 34 days, 7 weeks, and 20 months, respectively, because of unusual circumstances. The extracted teeth were processed, paraffin embedded, sectioned, stained with hematoxylin-eosin or the modified Brown and Brenn technique, and examined with light microscopy. The recurrent observation for the 3 cases presented was the absence of inflammatory or foreign body reactions of the host tissues in contact with tricalcium silicate–based materials after different observation periods despite the identification of bacteria in dentinal tubules close to the site of operation. Wound healing was rapid with repair/regeneration of lost tissues with cementum and new bone trabeculae. Although the level of evidence for a case series is low because of the anecdotal nature of the reported episodes, the histologic results reported in the present case series illustrate the highly biocompatible and bioactive nature of the tricalcium silicate–based materials used in treating these cases.
AB - Pulpal/periapical responses of human teeth that are treated successfully with tricalcium silicate–based materials are extremely difficult to obtain because of the typical unavailability of these teeth for histologic examination. The present case series reports histologic and histobacteriologic findings of 3 human teeth that had undergone pulpotomy, orthograde retreatment, and apicoectomy/root-end filling using tricalcium silicate–based endodontic materials. The teeth were extracted after 34 days, 7 weeks, and 20 months, respectively, because of unusual circumstances. The extracted teeth were processed, paraffin embedded, sectioned, stained with hematoxylin-eosin or the modified Brown and Brenn technique, and examined with light microscopy. The recurrent observation for the 3 cases presented was the absence of inflammatory or foreign body reactions of the host tissues in contact with tricalcium silicate–based materials after different observation periods despite the identification of bacteria in dentinal tubules close to the site of operation. Wound healing was rapid with repair/regeneration of lost tissues with cementum and new bone trabeculae. Although the level of evidence for a case series is low because of the anecdotal nature of the reported episodes, the histologic results reported in the present case series illustrate the highly biocompatible and bioactive nature of the tricalcium silicate–based materials used in treating these cases.
KW - Apical surgery
KW - EndoSequence
KW - orthograde retreatment
KW - partial pulpotomy
KW - tricalcium silicate–based material
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U2 - 10.1016/j.joen.2019.10.032
DO - 10.1016/j.joen.2019.10.032
M3 - Article
C2 - 31836137
AN - SCOPUS:85076475686
SN - 0099-2399
VL - 46
SP - 307
EP - 317
JO - Journal of endodontics
JF - Journal of endodontics
IS - 2
ER -