Histologic Response of Human Pulp and Periapical Tissues to Tricalcium Silicate–based Materials: A Series of Successfully Treated Cases

Domenico Ricucci, Nicola Maria Grande, Gianluca Plotino, Franklin R. Tay

Research output: Contribution to journalArticle

1 Scopus citations


Pulpal/periapical responses of human teeth that are treated successfully with tricalcium silicate–based materials are extremely difficult to obtain because of the typical unavailability of these teeth for histologic examination. The present case series reports histologic and histobacteriologic findings of 3 human teeth that had undergone pulpotomy, orthograde retreatment, and apicoectomy/root-end filling using tricalcium silicate–based endodontic materials. The teeth were extracted after 34 days, 7 weeks, and 20 months, respectively, because of unusual circumstances. The extracted teeth were processed, paraffin embedded, sectioned, stained with hematoxylin-eosin or the modified Brown and Brenn technique, and examined with light microscopy. The recurrent observation for the 3 cases presented was the absence of inflammatory or foreign body reactions of the host tissues in contact with tricalcium silicate–based materials after different observation periods despite the identification of bacteria in dentinal tubules close to the site of operation. Wound healing was rapid with repair/regeneration of lost tissues with cementum and new bone trabeculae. Although the level of evidence for a case series is low because of the anecdotal nature of the reported episodes, the histologic results reported in the present case series illustrate the highly biocompatible and bioactive nature of the tricalcium silicate–based materials used in treating these cases.

Original languageEnglish (US)
Pages (from-to)307-317
Number of pages11
JournalJournal of endodontics
Issue number2
StatePublished - Feb 2020



  • Apical surgery
  • EndoSequence
  • orthograde retreatment
  • partial pulpotomy
  • tricalcium silicate–based material

ASJC Scopus subject areas

  • Dentistry(all)

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