Hormonal and nutritional regulation of muscle carnitine palmitoyltransferase I gene expression in vivo

HongYan Liu, Guolu Zheng, Hongfa Zhu, Gebre Woldegiorgis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Transgenic mice carrying the human heart muscle carnitine palmitoyltransferase I (M-CPTI) gene fused to a CAT reporter gene were generated to study the regulation of M-CPTI gene expression. When the mice were fasted for 48 h, CAT activity and mRNA levels increased by more than 2-fold in heart and skeletal muscle, but not liver or kidney. In the diabetic transgenic mice, there was a 2- to 3-fold increase in CAT activity and CAT mRNA levels in heart and skeletal muscle which upon insulin administration reverted to that observed with the control insulin sufficient transgenic mice. Feeding a high fat diet increased CAT activity and mRNA levels by 2- to 4-fold in heart and skeletal muscle of the transgenic mice compared to the control transgenic mice on regular diet. Overall, the M-CPTI promoter was found to be necessary for the tissue-specific hormonal and dietary regulation of the gene expression.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume465
Issue number2
DOIs
StatePublished - Sep 15 2007

Fingerprint

Carnitine O-Palmitoyltransferase
Gene expression
Transgenic Mice
Muscle
Myocardium
Gene Expression
Muscles
Skeletal Muscle
Messenger RNA
Nutrition
Insulin
Genes
Gene Expression Regulation
High Fat Diet
Reporter Genes
Liver
Diet
Kidney
Fats
Tissue

Keywords

  • CAT
  • Dietary
  • Gene expression
  • Hormonal
  • M-CPTI
  • Promoter
  • Regulation
  • Transgenic mice

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Hormonal and nutritional regulation of muscle carnitine palmitoyltransferase I gene expression in vivo. / Liu, HongYan; Zheng, Guolu; Zhu, Hongfa; Woldegiorgis, Gebre.

In: Archives of Biochemistry and Biophysics, Vol. 465, No. 2, 15.09.2007, p. 437-442.

Research output: Contribution to journalArticle

Liu, HongYan ; Zheng, Guolu ; Zhu, Hongfa ; Woldegiorgis, Gebre. / Hormonal and nutritional regulation of muscle carnitine palmitoyltransferase I gene expression in vivo. In: Archives of Biochemistry and Biophysics. 2007 ; Vol. 465, No. 2. pp. 437-442.
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