Hormonal and nutritional regulation of muscle carnitine palmitoyltransferase I gene expression in vivo

Hong Yan Liu, Guolu Zheng, Hongfa Zhu, Gebre Woldegiorgis

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Transgenic mice carrying the human heart muscle carnitine palmitoyltransferase I (M-CPTI) gene fused to a CAT reporter gene were generated to study the regulation of M-CPTI gene expression. When the mice were fasted for 48 h, CAT activity and mRNA levels increased by more than 2-fold in heart and skeletal muscle, but not liver or kidney. In the diabetic transgenic mice, there was a 2- to 3-fold increase in CAT activity and CAT mRNA levels in heart and skeletal muscle which upon insulin administration reverted to that observed with the control insulin sufficient transgenic mice. Feeding a high fat diet increased CAT activity and mRNA levels by 2- to 4-fold in heart and skeletal muscle of the transgenic mice compared to the control transgenic mice on regular diet. Overall, the M-CPTI promoter was found to be necessary for the tissue-specific hormonal and dietary regulation of the gene expression.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume465
Issue number2
DOIs
StatePublished - Sep 15 2007

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Keywords

  • CAT
  • Dietary
  • Gene expression
  • Hormonal
  • M-CPTI
  • Promoter
  • Regulation
  • Transgenic mice

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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