How insulin binds: The B-chain α-helix contacts the L1 β-helix of the insulin receptor

Kun Huang, Bin Xu, Shi Quan Hu, Ying Chi Chu, Qing Xin Hua, Yan Qu, Biaoru Li, Shuhua Wang, Run Ying Wang, Satoe H. Nakagawa, Anne Mette Theede, Jonathan Whittaker, Pierre De Meyts, Panayotis G. Katsoyannis, Michael A. Weiss

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Binding of insulin to the insulin receptor plays a central role in the hormonal control of metabolism. Here, we investigate possible contact sites between the receptor and the conserved non-polar surface of the B-chain. Evidence is presented that two contiguous sites in an α-helix, Val B12 and TyrB16, contact the receptor. Chemical synthesis is exploited to obtain non-standard substitutions in an engineered monomer (DKP-insulin). Substitution of TyrB16 by an isosteric photo-activatable derivative (para-azido-phenylalanine) enables efficient cross-linking to the receptor. Such cross-linking is specific and maps to the L1 β-helix of the α-subunit. Because substitution of ValB12 by larger side-chains markedly impairs receptor binding, cross-linking studies at B12 were not undertaken. Structure-function relationships are instead probed by side-chains of similar or smaller volume: respective substitution of Val B12 by alanine, threonine, and α-aminobutyric acid leads to activities of 1(±0.1)%, 13(±6)%, and 14(±5)% (relative to DKP-insulin) without disproportionate changes in negative cooperativity. NMR structures are essentially identical with native insulin. The absence of transmitted structural changes suggests that the low activities of B12 analogues reflect local perturbation of a "high-affinity" hormone-receptor contact. By contrast, because position B16 tolerates alanine substitution (relative activity 34(±10)%), the contribution of this neighboring interaction is smaller. Together, our results support a model in which the B-chain α-helix, functioning as an essential recognition element, docks against the L1 β-helix of the insulin receptor.

Original languageEnglish (US)
Pages (from-to)529-550
Number of pages22
JournalJournal of Molecular Biology
Volume341
Issue number2
DOIs
StatePublished - Aug 6 2004

Keywords

  • Aba, α-aminobutyric acid
  • CD, circular dichroism
  • CHO, Chinese hamster ovary
  • CR, cysteine-rich domain of receptor α-subunit
  • DG, distance geometry
  • NMR spectroscopy
  • diabetes mellitus
  • metabolism
  • non-standard mutagenesis
  • polypeptide hormone

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'How insulin binds: The B-chain α-helix contacts the L1 β-helix of the insulin receptor'. Together they form a unique fingerprint.

  • Cite this

    Huang, K., Xu, B., Hu, S. Q., Chu, Y. C., Hua, Q. X., Qu, Y., Li, B., Wang, S., Wang, R. Y., Nakagawa, S. H., Theede, A. M., Whittaker, J., De Meyts, P., Katsoyannis, P. G., & Weiss, M. A. (2004). How insulin binds: The B-chain α-helix contacts the L1 β-helix of the insulin receptor. Journal of Molecular Biology, 341(2), 529-550. https://doi.org/10.1016/j.jmb.2004.05.023