Human interstitial retinoid-binding protein. Gene structure and primary sequence

Gregory I Liou, D. P. Ma, Y. W. Yang, L. Geng, C. Zhu, W. Baehr

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Interstitial retinoid-binding protein (IRBP) is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix and is known to bind retinoids and fatty acids. We have used cDNA clones encoding human IRBP to isolate a 15-kilobase genomic fragment that encompasses the complete human IRBP gene. The IRBP gene spans more than 11 kilobases and is interrupted by three introns, all of which are positioned near the 3'-end of the coding sequence. The 3741-base pair coding region of IRBP appears to have been generated by quadruplication of an approximately 900 base pair long ancestral gene. The deduced amino acid sequence predicts a mature protein of 1,230 residues (calculated molecular weight 133,000). The protein sequence can be aligned into four homologous segments, each consisting of about 300 residues. Sequence similarity between segments is as high as 60% when conservative substitutions are taken into account. Two putative N-linked glycosylation sites are located in highly conserved domains in the center of the first and second segment of IRBP. A domain consisting of 41 residues at the COOH-terminal end of the third segment has 15 matching residues (38%) with an intradiscal loop of rhodopsin, a retinal-binding protein in rod photoreceptors.

Original languageEnglish (US)
Pages (from-to)8200-8206
Number of pages7
JournalJournal of Biological Chemistry
Volume264
Issue number14
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Liou, G. I., Ma, D. P., Yang, Y. W., Geng, L., Zhu, C., & Baehr, W. (1989). Human interstitial retinoid-binding protein. Gene structure and primary sequence. Journal of Biological Chemistry, 264(14), 8200-8206.