Human lymphocytes synthesize C-reactive protein

Tohru Ikuta, Hideo Okubo, Hiromi Ishibashi, Yuzo Okumura, Kazuhiro Hayashida

Research output: Contribution to journalArticle

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Abstract

We obtained evidence for the synthesis and secretion of C-reactive protein (CRP) by peripheral mononuclear cells in culture. Human mononuclear cells isolated from peripheral blood, after depletion of platelets, were cultured in giutamine-depleted RPMI 1640 supplemented with [3H]glutamine in the presence of 10-O-tetradecanoyl-phorbol-13-acetate (TPA). Anti-CRP antiserum was added to the culture medium, and the resultant immunoprecipitate was analyzed in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The immunoprecipitate consisted of CRP, heavy and light chains of IgG, and only the CRP protein band had radioactivity, indicating that CRP was synthesized by mononuclear cells. In the populations of mononuciear cells, T-cell preparations mainly synthesized CRP, under stimulation of a factor derived from activated monocytes. Studies using the inhibitors of phospholipid metabolism suggested that generation of the monocyte factor was relevant to metabolites of an arachidonate cascade.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalInflammation
Volume10
Issue number3
DOIs
StatePublished - Sep 1 1986
Externally publishedYes

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C-Reactive Protein
Lymphocytes
Monocytes
Glutamine
Radioactivity
Culture Media
Immune Sera
Polyacrylamide Gel Electrophoresis
Phospholipids
Acetates
Blood Platelets
Cell Culture Techniques
Immunoglobulin G
T-Lymphocytes
Light
Population
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ikuta, T., Okubo, H., Ishibashi, H., Okumura, Y., & Hayashida, K. (1986). Human lymphocytes synthesize C-reactive protein. Inflammation, 10(3), 223-232. https://doi.org/10.1007/BF00916118

Human lymphocytes synthesize C-reactive protein. / Ikuta, Tohru; Okubo, Hideo; Ishibashi, Hiromi; Okumura, Yuzo; Hayashida, Kazuhiro.

In: Inflammation, Vol. 10, No. 3, 01.09.1986, p. 223-232.

Research output: Contribution to journalArticle

Ikuta, T, Okubo, H, Ishibashi, H, Okumura, Y & Hayashida, K 1986, 'Human lymphocytes synthesize C-reactive protein', Inflammation, vol. 10, no. 3, pp. 223-232. https://doi.org/10.1007/BF00916118
Ikuta T, Okubo H, Ishibashi H, Okumura Y, Hayashida K. Human lymphocytes synthesize C-reactive protein. Inflammation. 1986 Sep 1;10(3):223-232. https://doi.org/10.1007/BF00916118
Ikuta, Tohru ; Okubo, Hideo ; Ishibashi, Hiromi ; Okumura, Yuzo ; Hayashida, Kazuhiro. / Human lymphocytes synthesize C-reactive protein. In: Inflammation. 1986 ; Vol. 10, No. 3. pp. 223-232.
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