Human Photoreactivating Enzyme

Action Spectrum and Safelight Conditions

John C. Sutherland, B. M. Sutherland

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The action spectrum for photoreactivation by enzymes from human leukocytes and fibroblasts extends from 300 to approximately 600 nm with a maximum near 400 nm. The ability of the human enzymes to utilize light of wavelengths greater than 500 nm suggested that yellow or gold lights conventionally used as safelights for photoreactivation might serve as sources of photoreactivating light for these enzymes. Experiments using lights with a range of spectral outputs confirm that the standard yellow “safe” lights do produce photoreactivation by the human but not the Escherichia coli enzyme.

Original languageEnglish (US)
Pages (from-to)435-440
Number of pages6
JournalBiophysical Journal
Volume15
Issue number5
DOIs
StatePublished - Jan 1 1975

Fingerprint

Deoxyribodipyrimidine Photo-Lyase
Light
Enzymes
Gold
Leukocytes
Fibroblasts
Action Spectrum
Escherichia coli

ASJC Scopus subject areas

  • Biophysics

Cite this

Human Photoreactivating Enzyme : Action Spectrum and Safelight Conditions. / Sutherland, John C.; Sutherland, B. M.

In: Biophysical Journal, Vol. 15, No. 5, 01.01.1975, p. 435-440.

Research output: Contribution to journalArticle

@article{baee39ed5fe747d48e07dbda49f7a8c0,
title = "Human Photoreactivating Enzyme: Action Spectrum and Safelight Conditions",
abstract = "The action spectrum for photoreactivation by enzymes from human leukocytes and fibroblasts extends from 300 to approximately 600 nm with a maximum near 400 nm. The ability of the human enzymes to utilize light of wavelengths greater than 500 nm suggested that yellow or gold lights conventionally used as safelights for photoreactivation might serve as sources of photoreactivating light for these enzymes. Experiments using lights with a range of spectral outputs confirm that the standard yellow “safe” lights do produce photoreactivation by the human but not the Escherichia coli enzyme.",
author = "Sutherland, {John C.} and Sutherland, {B. M.}",
year = "1975",
month = "1",
day = "1",
doi = "10.1016/S0006-3495(75)85828-0",
language = "English (US)",
volume = "15",
pages = "435--440",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "5",

}

TY - JOUR

T1 - Human Photoreactivating Enzyme

T2 - Action Spectrum and Safelight Conditions

AU - Sutherland, John C.

AU - Sutherland, B. M.

PY - 1975/1/1

Y1 - 1975/1/1

N2 - The action spectrum for photoreactivation by enzymes from human leukocytes and fibroblasts extends from 300 to approximately 600 nm with a maximum near 400 nm. The ability of the human enzymes to utilize light of wavelengths greater than 500 nm suggested that yellow or gold lights conventionally used as safelights for photoreactivation might serve as sources of photoreactivating light for these enzymes. Experiments using lights with a range of spectral outputs confirm that the standard yellow “safe” lights do produce photoreactivation by the human but not the Escherichia coli enzyme.

AB - The action spectrum for photoreactivation by enzymes from human leukocytes and fibroblasts extends from 300 to approximately 600 nm with a maximum near 400 nm. The ability of the human enzymes to utilize light of wavelengths greater than 500 nm suggested that yellow or gold lights conventionally used as safelights for photoreactivation might serve as sources of photoreactivating light for these enzymes. Experiments using lights with a range of spectral outputs confirm that the standard yellow “safe” lights do produce photoreactivation by the human but not the Escherichia coli enzyme.

UR - http://www.scopus.com/inward/record.url?scp=0016704343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016704343&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(75)85828-0

DO - 10.1016/S0006-3495(75)85828-0

M3 - Article

VL - 15

SP - 435

EP - 440

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 5

ER -