Human placental sodium-dependent vitamin C transporter (SVCT2): Molecular cloning and transport function

D. Prasanna Rajan, Wei Huang, Binita Dutta, Lawrence D Devoe, Frederich H. Leibach, Vadivel Ganapathy, Puttur D Prasad

Research output: Contribution to journalArticle

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Abstract

We report here on the cloning and functional characterization of human SVCT2, a sodium-dependent vitamin C (ascorbate) transporter. The hSVCT2 cDNA obtained from a human placental choriocarcinoma cell cDNA library, codes for a protein of 650 amino acids with a predicted molecular mass of 70 kDa. At the level of amino acid sequence, the human SVCT2 exhibits 95% identity to its rat homolog. When functionally expressed in mammalian cells, hSVCT2 induces the transport of ascorbic acid. The transport process induced by hSVCT2 is Na+-dependent and is specific for ascorbate. The Michaelis-Menton constant (K(t)) for the transport of ascorbate in cDNA-transfected cells is 69 ± 5 μM. The relationship between the cDNA-specific uptake rate of ascorbate and Na+ concentration is sigmoidal with a Na+:ascorbate stoichiometry of 2:1. Northern blot analysis shows that SVCT2-specific transcripts are present in heart, brain, placenta, and liver and is absent in lung and skeletal muscle. The size of the principal transcript is ~ 7.5 kb.

Original languageEnglish (US)
Pages (from-to)762-768
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume262
Issue number3
DOIs
StatePublished - Sep 7 1999

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Sodium-Coupled Vitamin C Transporters
Cloning
Molecular Cloning
Complementary DNA
Ascorbic Acid
Amino Acids
Choriocarcinoma
Molecular mass
Gene Library
Stoichiometry
Northern Blotting
Liver
Placenta
Muscle
Rats
Organism Cloning
Amino Acid Sequence
Brain
Skeletal Muscle
Sodium

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Human placental sodium-dependent vitamin C transporter (SVCT2) : Molecular cloning and transport function. / Rajan, D. Prasanna; Huang, Wei; Dutta, Binita; Devoe, Lawrence D; Leibach, Frederich H.; Ganapathy, Vadivel; Prasad, Puttur D.

In: Biochemical and Biophysical Research Communications, Vol. 262, No. 3, 07.09.1999, p. 762-768.

Research output: Contribution to journalArticle

Rajan, D. Prasanna ; Huang, Wei ; Dutta, Binita ; Devoe, Lawrence D ; Leibach, Frederich H. ; Ganapathy, Vadivel ; Prasad, Puttur D. / Human placental sodium-dependent vitamin C transporter (SVCT2) : Molecular cloning and transport function. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 262, No. 3. pp. 762-768.
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