Human‐papillomavirus DNA in cholesteatomas

Krister Bergmann, Florian Hoppe, Yukai He, Jan Helms, Hans‐Konrad ‐K Müller‐Hermelink, Armin Stremlau, Ethel‐Michele ‐M De Villiers

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cholesteatoma of the middle ear is a relatively common disorder, often with severe consequences. Histologically, the aggressively growing, bone‐destructing form shows papillary growth and koilocytosis, which are characteristic of papillomavirus‐induced lesions. A PCR (polymerase chain reaction) method using degenerate primers for the detection of any known or as yet unknown HPV (human papillomavirus) type was applied in screening 51 biopsies from 42 patients. A resulting 36% (16/45) of the cholesteatomas were found to contain papillomavirus DNA, which hybridized under stringent conditions with an HPV‐II DNA probe. In 3 cases the presence of HPV‐II DNA could be confirmed by sequencing the PCR products. The mere presence of this HPV DNA does not prove an etiological role of this group of viruses in the induction of cholesteatomas. It does, however, identify another group of human proliferative lesions putatively linked to papillomavirus infections. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)463-466
Number of pages4
JournalInternational Journal of Cancer
Volume59
Issue number4
DOIs
StatePublished - Jan 1 1994

Fingerprint

Cholesteatoma
DNA
Middle Ear Cholesteatoma
Virus Activation
Polymerase Chain Reaction
Papillomavirus Infections
DNA Probes
Biopsy
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Bergmann, K., Hoppe, F., He, Y., Helms, J., Müller‐Hermelink, HK. K., Stremlau, A., & De Villiers, EM. M. (1994). Human‐papillomavirus DNA in cholesteatomas. International Journal of Cancer, 59(4), 463-466. https://doi.org/10.1002/ijc.2910590405

Human‐papillomavirus DNA in cholesteatomas. / Bergmann, Krister; Hoppe, Florian; He, Yukai; Helms, Jan; Müller‐Hermelink, Hans‐Konrad ‐K; Stremlau, Armin; De Villiers, Ethel‐Michele ‐M.

In: International Journal of Cancer, Vol. 59, No. 4, 01.01.1994, p. 463-466.

Research output: Contribution to journalArticle

Bergmann, K, Hoppe, F, He, Y, Helms, J, Müller‐Hermelink, HKK, Stremlau, A & De Villiers, EMM 1994, 'Human‐papillomavirus DNA in cholesteatomas', International Journal of Cancer, vol. 59, no. 4, pp. 463-466. https://doi.org/10.1002/ijc.2910590405
Bergmann K, Hoppe F, He Y, Helms J, Müller‐Hermelink HKK, Stremlau A et al. Human‐papillomavirus DNA in cholesteatomas. International Journal of Cancer. 1994 Jan 1;59(4):463-466. https://doi.org/10.1002/ijc.2910590405
Bergmann, Krister ; Hoppe, Florian ; He, Yukai ; Helms, Jan ; Müller‐Hermelink, Hans‐Konrad ‐K ; Stremlau, Armin ; De Villiers, Ethel‐Michele ‐M. / Human‐papillomavirus DNA in cholesteatomas. In: International Journal of Cancer. 1994 ; Vol. 59, No. 4. pp. 463-466.
@article{6a2753c9b57e477a9a2709362214ecbf,
title = "Human‐papillomavirus DNA in cholesteatomas",
abstract = "Cholesteatoma of the middle ear is a relatively common disorder, often with severe consequences. Histologically, the aggressively growing, bone‐destructing form shows papillary growth and koilocytosis, which are characteristic of papillomavirus‐induced lesions. A PCR (polymerase chain reaction) method using degenerate primers for the detection of any known or as yet unknown HPV (human papillomavirus) type was applied in screening 51 biopsies from 42 patients. A resulting 36{\%} (16/45) of the cholesteatomas were found to contain papillomavirus DNA, which hybridized under stringent conditions with an HPV‐II DNA probe. In 3 cases the presence of HPV‐II DNA could be confirmed by sequencing the PCR products. The mere presence of this HPV DNA does not prove an etiological role of this group of viruses in the induction of cholesteatomas. It does, however, identify another group of human proliferative lesions putatively linked to papillomavirus infections. {\circledC} 1994 Wiley‐Liss, Inc.",
author = "Krister Bergmann and Florian Hoppe and Yukai He and Jan Helms and M{\"u}ller‐Hermelink, {Hans‐Konrad ‐K} and Armin Stremlau and {De Villiers}, {Ethel‐Michele ‐M}",
year = "1994",
month = "1",
day = "1",
doi = "10.1002/ijc.2910590405",
language = "English (US)",
volume = "59",
pages = "463--466",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Human‐papillomavirus DNA in cholesteatomas

AU - Bergmann, Krister

AU - Hoppe, Florian

AU - He, Yukai

AU - Helms, Jan

AU - Müller‐Hermelink, Hans‐Konrad ‐K

AU - Stremlau, Armin

AU - De Villiers, Ethel‐Michele ‐M

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Cholesteatoma of the middle ear is a relatively common disorder, often with severe consequences. Histologically, the aggressively growing, bone‐destructing form shows papillary growth and koilocytosis, which are characteristic of papillomavirus‐induced lesions. A PCR (polymerase chain reaction) method using degenerate primers for the detection of any known or as yet unknown HPV (human papillomavirus) type was applied in screening 51 biopsies from 42 patients. A resulting 36% (16/45) of the cholesteatomas were found to contain papillomavirus DNA, which hybridized under stringent conditions with an HPV‐II DNA probe. In 3 cases the presence of HPV‐II DNA could be confirmed by sequencing the PCR products. The mere presence of this HPV DNA does not prove an etiological role of this group of viruses in the induction of cholesteatomas. It does, however, identify another group of human proliferative lesions putatively linked to papillomavirus infections. © 1994 Wiley‐Liss, Inc.

AB - Cholesteatoma of the middle ear is a relatively common disorder, often with severe consequences. Histologically, the aggressively growing, bone‐destructing form shows papillary growth and koilocytosis, which are characteristic of papillomavirus‐induced lesions. A PCR (polymerase chain reaction) method using degenerate primers for the detection of any known or as yet unknown HPV (human papillomavirus) type was applied in screening 51 biopsies from 42 patients. A resulting 36% (16/45) of the cholesteatomas were found to contain papillomavirus DNA, which hybridized under stringent conditions with an HPV‐II DNA probe. In 3 cases the presence of HPV‐II DNA could be confirmed by sequencing the PCR products. The mere presence of this HPV DNA does not prove an etiological role of this group of viruses in the induction of cholesteatomas. It does, however, identify another group of human proliferative lesions putatively linked to papillomavirus infections. © 1994 Wiley‐Liss, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0028127248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028127248&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910590405

DO - 10.1002/ijc.2910590405

M3 - Article

C2 - 7960213

AN - SCOPUS:0028127248

VL - 59

SP - 463

EP - 466

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -