ICAM-1 expression on human brain microvascular endothelial cells

David C Hess, Tiku Bhutwala, James C. Sheppard, Wei Zhao, Joseph Smith

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

There is evidence that leukocytes play an important role in mediating tissue injury during acute ischemic stroke. Endothelial cell adhesive molecules such as ICAM-1 are required for the migration of leukocytes into the brain. Using an Elisa, we compared the expression of ICAM-1 by human brain microvascular endothelial cells with human umbilical vein endothelial cells. There was constitutive surface expression of ICAM-1 on both brain and umbilical vein endothelial cells. With cytokine (IL-1β or TNF) or lipopolysaccaride stimulation, ICAM-1 surface expression increased to a greater extent on brain than on umbilical vein endothelial cells. Dexamethasone at doses up to 100 μM had no effect on inhibiting cytokine-mediated upregulation of ICAM-1 on human brain microvascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)201-204
Number of pages4
JournalNeuroscience Letters
Volume168
Issue number1-2
DOIs
StatePublished - Feb 28 1994

Keywords

  • Brain
  • Cerebrovascular, cytokine
  • Endothelial
  • ICAM-1

ASJC Scopus subject areas

  • Neuroscience(all)

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