ICAM-1 expression on human brain microvascular endothelial cells

David C. Hess, Tiku Bhutwala, James C. Sheppard, Wei Zhao, Joseph Smith

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

There is evidence that leukocytes play an important role in mediating tissue injury during acute ischemic stroke. Endothelial cell adhesive molecules such as ICAM-1 are required for the migration of leukocytes into the brain. Using an Elisa, we compared the expression of ICAM-1 by human brain microvascular endothelial cells with human umbilical vein endothelial cells. There was constitutive surface expression of ICAM-1 on both brain and umbilical vein endothelial cells. With cytokine (IL-1β or TNF) or lipopolysaccaride stimulation, ICAM-1 surface expression increased to a greater extent on brain than on umbilical vein endothelial cells. Dexamethasone at doses up to 100 μM had no effect on inhibiting cytokine-mediated upregulation of ICAM-1 on human brain microvascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)201-204
Number of pages4
JournalNeuroscience Letters
Volume168
Issue number1-2
DOIs
StatePublished - Feb 28 1994

Keywords

  • Brain
  • Cerebrovascular, cytokine
  • Endothelial
  • ICAM-1

ASJC Scopus subject areas

  • Neuroscience(all)

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