Identification and characterization of a Streptococcus pyogenes operon involved in binding of hemoproteins and acquisition of iron

Christopher S. Bates, Griselle E. Montañez, Charles R. Woods, Rebecca M. Vincent, Zehava Eichenbaum

Research output: Contribution to journalArticle

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Abstract

The hemolytic Streptococcus pyogenes can use a variety of heme compounds as an iron source. In this study, we investigate hemoprotein utilization by S. pyogenes. We demonstrate that surface proteins contribute to the binding of hemoproteins to S. pyogenes. We identify an ABC transporter from the iron complex family named sia for streptococcal iron acquisition, which consists of a lipoprotein (siaA), membrane permease (siaB), and ATPase (siaC). The sia transporter is part of a highly conserved, iron regulated, 10-gene operon. SiaA, which was localized to the cell membrane, could specifically bind hemoglobin. The operon's first gene encodes a novel bacterial protein that bound hemoglobin, myoglobin, heme-albumin, and hemoglobin-haptoglobin (but not apo-haptoglobin) and therefore was named Shr, for streptococcal hemoprotein receptor. PhoZ fusion and Western blot analysis showed that Shr has a leader peptide and is found in both membrane-bound and soluble forms. An M1 SF370 strain with a polar mutation in shr was more resistant to streptonigrin and hydrogen peroxide, suggesting decreased iron uptake. The addition of hemoglobin to the culture medium increased cell resistance to hydrogen peroxide in SF370 but not in the mutant, implying the sia operon may be involved in hemoglobin-dependent resistance to oxidative stress. The shr mutant demonstrated reduced hemoglobin binding, though cell growth in iron-depleted medium supplemented with hemoglobin, whole blood, or ferric citrate was not affected, suggesting additional systems are involved in hemoglobin utilization. SiaA and Shr are the first hemoprotein receptors identified in S. pyogenes; their possible role in iron capture is discussed.

Original languageEnglish (US)
Pages (from-to)1042-1055
Number of pages14
JournalInfection and Immunity
Volume71
Issue number3
DOIs
StatePublished - Mar 1 2003

Fingerprint

Streptococcus pyogenes
Operon
Hemoglobins
Iron
Haptoglobins
Heme
Hydrogen Peroxide
Streptonigrin
Iron Compounds
ATP-Binding Cassette Transporters
Bacterial Proteins
Membranes
Membrane Transport Proteins
Myoglobin
Protein Sorting Signals
Genes
Lipoproteins
Culture Media
Adenosine Triphosphatases
Albumins

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Identification and characterization of a Streptococcus pyogenes operon involved in binding of hemoproteins and acquisition of iron. / Bates, Christopher S.; Montañez, Griselle E.; Woods, Charles R.; Vincent, Rebecca M.; Eichenbaum, Zehava.

In: Infection and Immunity, Vol. 71, No. 3, 01.03.2003, p. 1042-1055.

Research output: Contribution to journalArticle

Bates, Christopher S. ; Montañez, Griselle E. ; Woods, Charles R. ; Vincent, Rebecca M. ; Eichenbaum, Zehava. / Identification and characterization of a Streptococcus pyogenes operon involved in binding of hemoproteins and acquisition of iron. In: Infection and Immunity. 2003 ; Vol. 71, No. 3. pp. 1042-1055.
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