Identification and Characterization of A T-Helper Peptide from Carcinoembryonic Antigen

Marta Ruiz, Hiroya Kobayashi, Juan José Lasarte, Jesús Prieto, Francisco Borrás-Cuesta, Esteban Celis, Pablo Sarobe

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Purpose: The purpose of this research was to identify promiscuous T-helper cell determinants (THd) from carcinoembryonic antigen (CEA) to be used to prime T-cell help for cancer therapy. CEA was selected because this antigen is expressed in an important variety of carcinomas. Experimental Design: Potential promiscuous THd from CEA were predicted using available computer algorithms. Predicted peptides were synthesized and tested in binding experiments to different HLA-DR molecules. Binder peptides were then used to prime T-cell responses both in vitro and in vivo. Results: Twenty 15-mer peptides from CEA were predicted to bind to different HLA-DR molecules. The promiscuous character of these peptides was demonstrated in binding experiments. Fifteen of 20 peptides tested were able to bind to HLA-DR4, but only CEA (625-639) was shown to be presented after processing of recombinant CEA. CEA (625-639) was also found to be presented by HLA-DR53. Moreover, immunization of HLA-DR4 transgenic mice with CEA (625-639) in conjunction with class I epitope OVA (257-264), induced a CTL response specific of OVA (257-264). Conclusions: CEA (625-639) might be a relevant promiscuous THd peptide for cancer therapy.

Original languageEnglish (US)
Pages (from-to)2860-2867
Number of pages8
JournalClinical Cancer Research
Volume10
Issue number8
DOIs
StatePublished - Apr 15 2004
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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