Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila

Nita Jane Maihle, Birgit H. Satir

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryoticcell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [1] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.

Original languageEnglish (US)
Pages (from-to)195-202
Number of pages8
JournalJournal of Cellular Biochemistry
Volume31
Issue number3
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Tetrahymena thermophila
Secretory Pathway
Biomarkers
Tetrahymena
Peptides
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Optical microscopy
Immune Sera
Carrier Proteins
Proteins
Research Personnel
Light

Keywords

  • Tetrahymena
  • ciliates
  • protein secretion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila. / Maihle, Nita Jane; Satir, Birgit H.

In: Journal of Cellular Biochemistry, Vol. 31, No. 3, 01.01.1986, p. 195-202.

Research output: Contribution to journalArticle

Maihle, Nita Jane ; Satir, Birgit H. / Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila. In: Journal of Cellular Biochemistry. 1986 ; Vol. 31, No. 3. pp. 195-202.
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