Abstract
Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryoticcell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [1] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 195-202 |
| Number of pages | 8 |
| Journal | Journal of Cellular Biochemistry |
| Volume | 31 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1986 |
| Externally published | Yes |
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Keywords
- Tetrahymena
- ciliates
- protein secretion
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila. / Maihle, Nita Jane; Satir, Birgit H.
In: Journal of Cellular Biochemistry, Vol. 31, No. 3, 01.01.1986, p. 195-202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila
AU - Maihle, Nita Jane
AU - Satir, Birgit H.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryoticcell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [1] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.
AB - Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryoticcell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [1] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.
KW - Tetrahymena
KW - ciliates
KW - protein secretion
UR - http://www.scopus.com/inward/record.url?scp=0022821735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022821735&partnerID=8YFLogxK
U2 - 10.1002/jcb.240310302
DO - 10.1002/jcb.240310302
M3 - Article
C2 - 3153454
AN - SCOPUS:0022821735
VL - 31
SP - 195
EP - 202
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 3
ER -