Abstract
Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryoticcell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [1] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 195-202 |
| Number of pages | 8 |
| Journal | Journal of Cellular Biochemistry |
| Volume | 31 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1986 |
| Externally published | Yes |
Keywords
- Tetrahymena
- ciliates
- protein secretion
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
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Maihle, N. J., & Satir, B. H. (1986). Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila. Journal of Cellular Biochemistry, 31(3), 195-202. https://doi.org/10.1002/jcb.240310302