Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila

Nita Jane Maihle, Birgit H. Satir

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Abstract

Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryoticcell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [1] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.

Original languageEnglish (US)
Pages (from-to)195-202
Number of pages8
JournalJournal of Cellular Biochemistry
Volume31
Issue number3
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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Keywords

  • Tetrahymena
  • ciliates
  • protein secretion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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