Identification of a rabies virus T cell epitope on the basis of its similarity with a hepatitis B surface antigen peptide presented to T cells by the same MHC molecule (HLA-DPw4)

Esteban Celis, J. Larson, L. Otvos, W. H. Wunner

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19 Citations (Scopus)

Abstract

The antigenic determinant recognized by a HLA-DPw4-restricted human T cell clone specific for rabies virus was identified by using a vaccinia-rabies nonstructural phosphoprotein recombinant virus and synthetic peptides of the sequence of rabies nonstructural Ag. These peptides were selected on the basis of three models that predict T cell epitopes. The antigenic determinant recognized by the rabies virus-specific T cell clone contained a five-amino acid segment highly homologous to a sequence found in a hepatitis B surface Ag epitope that stimulates human T cells in the context of the HLA-DPw4. A preliminary model of DPw4-restricted T cell determinants is elaborated based on a hypothesis of how the 2 α-helical peptides may bind to this MHC molecule. Results are further discussed in the context of the usefulness in identifying DPw4-restricted T cell epitopes for the production of synthetic vaccines because this MHC class II molecule is found with high frequency in the population.

Original languageEnglish (US)
Pages (from-to)305-310
Number of pages6
JournalJournal of Immunology
Volume145
Issue number1
StatePublished - Jan 1 1990
Externally publishedYes

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Rabies virus
T-Lymphocyte Epitopes
Hepatitis B Surface Antigens
T-Lymphocytes
Epitopes
Peptides
Rabies
Clone Cells
Vaccinia
Synthetic Vaccines
Phosphoproteins
Hepatitis B
Viruses
Amino Acids
HLA-DPw4 antigen
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

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abstract = "The antigenic determinant recognized by a HLA-DPw4-restricted human T cell clone specific for rabies virus was identified by using a vaccinia-rabies nonstructural phosphoprotein recombinant virus and synthetic peptides of the sequence of rabies nonstructural Ag. These peptides were selected on the basis of three models that predict T cell epitopes. The antigenic determinant recognized by the rabies virus-specific T cell clone contained a five-amino acid segment highly homologous to a sequence found in a hepatitis B surface Ag epitope that stimulates human T cells in the context of the HLA-DPw4. A preliminary model of DPw4-restricted T cell determinants is elaborated based on a hypothesis of how the 2 α-helical peptides may bind to this MHC molecule. Results are further discussed in the context of the usefulness in identifying DPw4-restricted T cell epitopes for the production of synthetic vaccines because this MHC class II molecule is found with high frequency in the population.",
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