Identification of CD15 as a Marker for Tumor-Propagating Cells in a Mouse Model of Medulloblastoma

Tracy Ann Read; Marie P. Fogarty; Shirley L. Markant; Roger E. McLendon; Zhengzheng Wei; David W. Ellison; Phillip G. Febbo; Robert J. Wechsler-Reya

doi:10.1016/j.ccr.2008.12.016

Identification of CD15 as a Marker for Tumor-Propagating Cells in a Mouse Model of Medulloblastoma

Tracy Ann Read, Marie P. Fogarty, Shirley L. Markant, Roger E. McLendon, Zhengzheng Wei, David W. Ellison, Phillip G. Febbo, Robert J. Wechsler-Reya

Research output: Contribution to journal › Article › peer-review

315 Scopus citations

Abstract

The growth of many cancers depends on self-renewing cells called cancer stem cells or tumor-propagating cells (TPCs). In human brain tumors, cells expressing the stem cell marker CD133 have been implicated as TPCs. Here we show that tumors from a model of medulloblastoma, the Patched mutant mouse, are propagated not by CD133⁺ cells but by cells expressing the progenitor markers Math1 and CD15/SSEA-1. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastomas, and tumors expressing genes similar to those found in murine CD15⁺ cells have a poorer prognosis. Thus, CD15 may represent an important marker for TPCs in medulloblastoma.

Original language	English (US)
Pages (from-to)	135-147
Number of pages	13
Journal	Cancer Cell
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/j.ccr.2008.12.016
State	Published - Feb 3 2009
Externally published	Yes

Keywords

CELLCYCLE
STEMCELL

ASJC Scopus subject areas

Oncology
Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ccr.2008.12.016

Cite this

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title = "Identification of CD15 as a Marker for Tumor-Propagating Cells in a Mouse Model of Medulloblastoma",

abstract = "The growth of many cancers depends on self-renewing cells called cancer stem cells or tumor-propagating cells (TPCs). In human brain tumors, cells expressing the stem cell marker CD133 have been implicated as TPCs. Here we show that tumors from a model of medulloblastoma, the Patched mutant mouse, are propagated not by CD133+ cells but by cells expressing the progenitor markers Math1 and CD15/SSEA-1. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastomas, and tumors expressing genes similar to those found in murine CD15+ cells have a poorer prognosis. Thus, CD15 may represent an important marker for TPCs in medulloblastoma.",

keywords = "CELLCYCLE, STEMCELL",

author = "Read, {Tracy Ann} and Fogarty, {Marie P.} and Markant, {Shirley L.} and McLendon, {Roger E.} and Zhengzheng Wei and Ellison, {David W.} and Febbo, {Phillip G.} and Wechsler-Reya, {Robert J.}",

note = "Funding Information: The authors thank J. Dutton for assistance with animal colony maintenance, B. Harvat for help with flow cytometry, T. Reya for helpful discussions, and J. Rich for critical review of the manuscript. We are also grateful to T. Contente-Cuomo and E. Salomon at the NIH Neuroscience Microarray Consortium at the Translational Genomics Research Institute for microarray processing and analysis. This work was supported by a fellowship from the University of Bergen (T.-A.R.) and by funds from the Duke Stem Cell Research Program, the Pediatric Brain Tumor Foundation, Golfers Against Cancer, and National Institute of Neurological Disorders and Stroke grant number NS052323-01 (R.J.W.-R.). ",

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T1 - Identification of CD15 as a Marker for Tumor-Propagating Cells in a Mouse Model of Medulloblastoma

AU - Read, Tracy Ann

AU - Fogarty, Marie P.

AU - Markant, Shirley L.

AU - McLendon, Roger E.

AU - Wei, Zhengzheng

AU - Ellison, David W.

AU - Febbo, Phillip G.

AU - Wechsler-Reya, Robert J.

N1 - Funding Information: The authors thank J. Dutton for assistance with animal colony maintenance, B. Harvat for help with flow cytometry, T. Reya for helpful discussions, and J. Rich for critical review of the manuscript. We are also grateful to T. Contente-Cuomo and E. Salomon at the NIH Neuroscience Microarray Consortium at the Translational Genomics Research Institute for microarray processing and analysis. This work was supported by a fellowship from the University of Bergen (T.-A.R.) and by funds from the Duke Stem Cell Research Program, the Pediatric Brain Tumor Foundation, Golfers Against Cancer, and National Institute of Neurological Disorders and Stroke grant number NS052323-01 (R.J.W.-R.).

PY - 2009/2/3

Y1 - 2009/2/3

N2 - The growth of many cancers depends on self-renewing cells called cancer stem cells or tumor-propagating cells (TPCs). In human brain tumors, cells expressing the stem cell marker CD133 have been implicated as TPCs. Here we show that tumors from a model of medulloblastoma, the Patched mutant mouse, are propagated not by CD133+ cells but by cells expressing the progenitor markers Math1 and CD15/SSEA-1. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastomas, and tumors expressing genes similar to those found in murine CD15+ cells have a poorer prognosis. Thus, CD15 may represent an important marker for TPCs in medulloblastoma.

AB - The growth of many cancers depends on self-renewing cells called cancer stem cells or tumor-propagating cells (TPCs). In human brain tumors, cells expressing the stem cell marker CD133 have been implicated as TPCs. Here we show that tumors from a model of medulloblastoma, the Patched mutant mouse, are propagated not by CD133+ cells but by cells expressing the progenitor markers Math1 and CD15/SSEA-1. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastomas, and tumors expressing genes similar to those found in murine CD15+ cells have a poorer prognosis. Thus, CD15 may represent an important marker for TPCs in medulloblastoma.

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Identification of CD15 as a Marker for Tumor-Propagating Cells in a Mouse Model of Medulloblastoma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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