Immature myeloid cells directly contribute to skin tumor development by recruiting IL-17-producing CD4+ T cells

Myrna L. Ortiz, Vinit Kumar, Anna Martner, Sridevi Mony, Laxminarasimha Donthireddy, Thomas Condamine, John Seykora, Stella C. Knight, George Malietzis, Gui Han Lee, Morgan Moorghen, Brianna Lenox, Noreen Luetteke, Esteban Celis, Dmitry Gabrilovich

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Evidence links chronic inflammation with cancer, but cellular mechanisms involved in this process remain unclear. We have demonstrated that in humans, inflammatory conditions that predispose to development of skin and colon tumors are associated with accumulation in tissues of CD33+S100A9+ cells, the phenotype typical for myeloid-derived suppressor cells in cancer or immature myeloid cells (IMCs) in tumor-free hosts. To identify the direct role of these cells in tumor development, we used S100A9 transgenic mice to create the conditions for topical accumulation of these cells in the skin in the absence of infection or tissue damage. These mice demonstrated accumulation of granulocytic IMCs in the skin upon topical application of 12-O-tetradecanoylphorbol-13-acetate (TPA), resulting in a dramatic increase in the formation of papillomas during epidermal carcinogenesis. The effect of IMCs on tumorigenesis was not associated with immune suppression, but with CCL4 (chemokine [C-C motif] ligand 4)-mediated recruitment of IL-17-producing CD4+ T cells. This chemokine was released by activated IMCs. Elimination of CD4+ T cells or blockade of CCL4 or IL-17 abrogated the increase in tumor formation caused by myeloid cells. Thus, this study implicates accumulation of IMCs as an initial step in facilitation of tumor formation, followed by the recruitment of CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)351-367
Number of pages17
JournalJournal of Experimental Medicine
Volume212
Issue number3
DOIs
StatePublished - Jan 1 2015

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Interleukin-17
Myeloid Cells
T-Lymphocytes
Skin
Neoplasms
CC Chemokines
Carcinogenesis
Ligands
Papilloma
Tetradecanoylphorbol Acetate
Chemokines
Transgenic Mice
Colon
Inflammation
Phenotype
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ortiz, M. L., Kumar, V., Martner, A., Mony, S., Donthireddy, L., Condamine, T., ... Gabrilovich, D. (2015). Immature myeloid cells directly contribute to skin tumor development by recruiting IL-17-producing CD4+ T cells. Journal of Experimental Medicine, 212(3), 351-367. https://doi.org/10.1084/jem.20140835

Immature myeloid cells directly contribute to skin tumor development by recruiting IL-17-producing CD4+ T cells. / Ortiz, Myrna L.; Kumar, Vinit; Martner, Anna; Mony, Sridevi; Donthireddy, Laxminarasimha; Condamine, Thomas; Seykora, John; Knight, Stella C.; Malietzis, George; Lee, Gui Han; Moorghen, Morgan; Lenox, Brianna; Luetteke, Noreen; Celis, Esteban; Gabrilovich, Dmitry.

In: Journal of Experimental Medicine, Vol. 212, No. 3, 01.01.2015, p. 351-367.

Research output: Contribution to journalArticle

Ortiz, ML, Kumar, V, Martner, A, Mony, S, Donthireddy, L, Condamine, T, Seykora, J, Knight, SC, Malietzis, G, Lee, GH, Moorghen, M, Lenox, B, Luetteke, N, Celis, E & Gabrilovich, D 2015, 'Immature myeloid cells directly contribute to skin tumor development by recruiting IL-17-producing CD4+ T cells', Journal of Experimental Medicine, vol. 212, no. 3, pp. 351-367. https://doi.org/10.1084/jem.20140835
Ortiz, Myrna L. ; Kumar, Vinit ; Martner, Anna ; Mony, Sridevi ; Donthireddy, Laxminarasimha ; Condamine, Thomas ; Seykora, John ; Knight, Stella C. ; Malietzis, George ; Lee, Gui Han ; Moorghen, Morgan ; Lenox, Brianna ; Luetteke, Noreen ; Celis, Esteban ; Gabrilovich, Dmitry. / Immature myeloid cells directly contribute to skin tumor development by recruiting IL-17-producing CD4+ T cells. In: Journal of Experimental Medicine. 2015 ; Vol. 212, No. 3. pp. 351-367.
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