Immune-mediated nephropathy and systemic autoimmunity in mice does not require receptor interacting protein Kinase 3 (RIPK3)

Chelsea Corradetti, Neelakshi R. Jog, Stefania Gallucci, Michael P. Madaio, Siddharth Balachandran, Roberto Caricchio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Immune mediated nephropathy is one of the most serious manifestations of lupus and is characterized by severe inflammation and necrosis that, if untreated, eventually leads to renal failure. Although lupus has a higher incidence in women, both sexes can develop lupus glomerulonephritis; nephritis in men develops earlier and is more severe than in women. It is therefore important to understand the cellular and molecular mechanisms mediating nephritis in each sex. Previous work by our lab found that the absence or pharmacological inhibition of Poly [ADP-ribose] polymerase 1 (PARP-1), an enzyme involved in DNA repair and necrotic cell death, affects only male mice and results in milder nephritis, with less in situ inflammation, and diminished incidence of necrotic lesions, allowing for higher survival rates. A second pathway mediating necrosis involves Receptor-Interacting Serine-Threonine Kinase 3 (RIPK3); in this study we sought to investigate the impact of RIPK3 on the development of lupus and nephritis in both sexes. To this end, we used two inducible murine models of lupus: chronic graft versus host disease (cGvHD) and pristaneinduced lupus; and nephrotoxic serum (NTS)-induced nephritis as a model of immune mediated nephropathy. We found that the absence of RIPK3 has neither positive nor negative impact on the disease development or progression of lupus and nephritis in all three models, and in both male and female mice. We conclude that RIPK3 is dispensable for the pathogenesis of lupus and immune mediated nephropathy as to accelerate, worsen or ameliorate the disease.

Original languageEnglish (US)
Article numbere0163611
JournalPloS one
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Receptor-Interacting Protein Serine-Threonine Kinases
nephritis
autoimmunity
Nephritis
kidney diseases
Autoimmunity
protein kinases
Protein Kinases
Lupus Nephritis
receptors
mice
Necrosis
Inflammation
Poly(ADP-ribose) Polymerases
gender
Protein-Serine-Threonine Kinases
Incidence
necrosis
Graft vs Host Disease
Cell death

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Corradetti, C., Jog, N. R., Gallucci, S., Madaio, M. P., Balachandran, S., & Caricchio, R. (2016). Immune-mediated nephropathy and systemic autoimmunity in mice does not require receptor interacting protein Kinase 3 (RIPK3). PloS one, 11(9), [e0163611]. https://doi.org/10.1371/journal.pone.0163611

Immune-mediated nephropathy and systemic autoimmunity in mice does not require receptor interacting protein Kinase 3 (RIPK3). / Corradetti, Chelsea; Jog, Neelakshi R.; Gallucci, Stefania; Madaio, Michael P.; Balachandran, Siddharth; Caricchio, Roberto.

In: PloS one, Vol. 11, No. 9, e0163611, 01.09.2016.

Research output: Contribution to journalArticle

Corradetti, C, Jog, NR, Gallucci, S, Madaio, MP, Balachandran, S & Caricchio, R 2016, 'Immune-mediated nephropathy and systemic autoimmunity in mice does not require receptor interacting protein Kinase 3 (RIPK3)', PloS one, vol. 11, no. 9, e0163611. https://doi.org/10.1371/journal.pone.0163611
Corradetti, Chelsea ; Jog, Neelakshi R. ; Gallucci, Stefania ; Madaio, Michael P. ; Balachandran, Siddharth ; Caricchio, Roberto. / Immune-mediated nephropathy and systemic autoimmunity in mice does not require receptor interacting protein Kinase 3 (RIPK3). In: PloS one. 2016 ; Vol. 11, No. 9.
@article{3f264f9236204210bf3ecf72542bb3bc,
title = "Immune-mediated nephropathy and systemic autoimmunity in mice does not require receptor interacting protein Kinase 3 (RIPK3)",
abstract = "Immune mediated nephropathy is one of the most serious manifestations of lupus and is characterized by severe inflammation and necrosis that, if untreated, eventually leads to renal failure. Although lupus has a higher incidence in women, both sexes can develop lupus glomerulonephritis; nephritis in men develops earlier and is more severe than in women. It is therefore important to understand the cellular and molecular mechanisms mediating nephritis in each sex. Previous work by our lab found that the absence or pharmacological inhibition of Poly [ADP-ribose] polymerase 1 (PARP-1), an enzyme involved in DNA repair and necrotic cell death, affects only male mice and results in milder nephritis, with less in situ inflammation, and diminished incidence of necrotic lesions, allowing for higher survival rates. A second pathway mediating necrosis involves Receptor-Interacting Serine-Threonine Kinase 3 (RIPK3); in this study we sought to investigate the impact of RIPK3 on the development of lupus and nephritis in both sexes. To this end, we used two inducible murine models of lupus: chronic graft versus host disease (cGvHD) and pristaneinduced lupus; and nephrotoxic serum (NTS)-induced nephritis as a model of immune mediated nephropathy. We found that the absence of RIPK3 has neither positive nor negative impact on the disease development or progression of lupus and nephritis in all three models, and in both male and female mice. We conclude that RIPK3 is dispensable for the pathogenesis of lupus and immune mediated nephropathy as to accelerate, worsen or ameliorate the disease.",
author = "Chelsea Corradetti and Jog, {Neelakshi R.} and Stefania Gallucci and Madaio, {Michael P.} and Siddharth Balachandran and Roberto Caricchio",
year = "2016",
month = "9",
day = "1",
doi = "10.1371/journal.pone.0163611",
language = "English (US)",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - Immune-mediated nephropathy and systemic autoimmunity in mice does not require receptor interacting protein Kinase 3 (RIPK3)

AU - Corradetti, Chelsea

AU - Jog, Neelakshi R.

AU - Gallucci, Stefania

AU - Madaio, Michael P.

AU - Balachandran, Siddharth

AU - Caricchio, Roberto

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Immune mediated nephropathy is one of the most serious manifestations of lupus and is characterized by severe inflammation and necrosis that, if untreated, eventually leads to renal failure. Although lupus has a higher incidence in women, both sexes can develop lupus glomerulonephritis; nephritis in men develops earlier and is more severe than in women. It is therefore important to understand the cellular and molecular mechanisms mediating nephritis in each sex. Previous work by our lab found that the absence or pharmacological inhibition of Poly [ADP-ribose] polymerase 1 (PARP-1), an enzyme involved in DNA repair and necrotic cell death, affects only male mice and results in milder nephritis, with less in situ inflammation, and diminished incidence of necrotic lesions, allowing for higher survival rates. A second pathway mediating necrosis involves Receptor-Interacting Serine-Threonine Kinase 3 (RIPK3); in this study we sought to investigate the impact of RIPK3 on the development of lupus and nephritis in both sexes. To this end, we used two inducible murine models of lupus: chronic graft versus host disease (cGvHD) and pristaneinduced lupus; and nephrotoxic serum (NTS)-induced nephritis as a model of immune mediated nephropathy. We found that the absence of RIPK3 has neither positive nor negative impact on the disease development or progression of lupus and nephritis in all three models, and in both male and female mice. We conclude that RIPK3 is dispensable for the pathogenesis of lupus and immune mediated nephropathy as to accelerate, worsen or ameliorate the disease.

AB - Immune mediated nephropathy is one of the most serious manifestations of lupus and is characterized by severe inflammation and necrosis that, if untreated, eventually leads to renal failure. Although lupus has a higher incidence in women, both sexes can develop lupus glomerulonephritis; nephritis in men develops earlier and is more severe than in women. It is therefore important to understand the cellular and molecular mechanisms mediating nephritis in each sex. Previous work by our lab found that the absence or pharmacological inhibition of Poly [ADP-ribose] polymerase 1 (PARP-1), an enzyme involved in DNA repair and necrotic cell death, affects only male mice and results in milder nephritis, with less in situ inflammation, and diminished incidence of necrotic lesions, allowing for higher survival rates. A second pathway mediating necrosis involves Receptor-Interacting Serine-Threonine Kinase 3 (RIPK3); in this study we sought to investigate the impact of RIPK3 on the development of lupus and nephritis in both sexes. To this end, we used two inducible murine models of lupus: chronic graft versus host disease (cGvHD) and pristaneinduced lupus; and nephrotoxic serum (NTS)-induced nephritis as a model of immune mediated nephropathy. We found that the absence of RIPK3 has neither positive nor negative impact on the disease development or progression of lupus and nephritis in all three models, and in both male and female mice. We conclude that RIPK3 is dispensable for the pathogenesis of lupus and immune mediated nephropathy as to accelerate, worsen or ameliorate the disease.

UR - http://www.scopus.com/inward/record.url?scp=84992179555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992179555&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0163611

DO - 10.1371/journal.pone.0163611

M3 - Article

AN - SCOPUS:84992179555

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e0163611

ER -