Immune suppression attenuates hypertension and renal disease in the Dahl salt-sensitive rat

David L. Mattson, Leilani James, Elizabeth A. Berdan, Carla J. Meister

Research output: Contribution to journalArticle

Abstract

Experiments were performed to determine the importance of activation or infiltration of immune cells in the kidney during the development of hypertension and renal disease in Dahl salt-sensitive rats (SS/Mcw) fed a 4.0% NaCl diet. Compared with vehicle-treated rats, chronic administration of mycophenolate mofetil ([MMF] 30 mg/kg per day, IP), an immunosuppressive agent that has cytostatic effects on T and B cells, decreased cell-specific markers of T and B cells by 50% to 60% in the kidneys of SS/Mcw rats (n=5 per group). Further studies were performed on Dahl SS/Mcw rats, which were instrumented with chronic indwelling catheters and studied after 3 weeks on the 4.0% NaCl diet. Rats were administered MMF or 5% dextrose vehicle daily during the 3-week period of high NaCl intake. Mean arterial blood pressure in the rats administered MMF (122±2 mm Hg; n=11) was significantly decreased compared with vehicle-treated rats (139±4 mm Hg; n=9). Furthermore, the rate of protein (112±13 mg per day) and albumin excretion (15±3 mg per day) in the MMF-treated rats was significantly lower than the protein and albumin excretion rate in vehicle-treated rats (167±25 and 31±7 mg per day, respectively). Creatinine clearance and body weight were not different between the groups, averaging 0.52±0.08 mL/min per gram kidney weight and 322±10 g, respectively, in the MMF-treated group. These experiments indicate that the activation of the immune system or renal infiltration of immune cells plays an important role in the development of hypertension and renal disease in Dahl SS/Mcw rats consuming an elevated NaCl diet.

Original languageEnglish (US)
Pages (from-to)149-156
Number of pages8
JournalHypertension
Volume48
Issue number1
DOIs
StatePublished - Jul 1 2006

Fingerprint

Inbred Dahl Rats
Renal Hypertension
Mycophenolic Acid
Kidney
Diet
Albumins
Arterial Pressure
B-Lymphocytes
T-Lymphocytes
Indwelling Catheters
Cytostatic Agents
Immunosuppressive Agents
Immune System
Creatinine
Proteins
Body Weight
Weights and Measures
Glucose

Keywords

  • Blood pressure
  • Hypertension
  • Rats
  • Sodium-dependent

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Immune suppression attenuates hypertension and renal disease in the Dahl salt-sensitive rat. / Mattson, David L.; James, Leilani; Berdan, Elizabeth A.; Meister, Carla J.

In: Hypertension, Vol. 48, No. 1, 01.07.2006, p. 149-156.

Research output: Contribution to journalArticle

Mattson, David L. ; James, Leilani ; Berdan, Elizabeth A. ; Meister, Carla J. / Immune suppression attenuates hypertension and renal disease in the Dahl salt-sensitive rat. In: Hypertension. 2006 ; Vol. 48, No. 1. pp. 149-156.
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