Immunohistochemical analysis and Epstein-Barr virus in the tonsils of transplant recipients and healthy controls

Sarah E. Mowry, Ali M. Strocker, Jessica Chan, Christopher Takehana, Nader Kalantar, Sunita Bhuta, Nina L. Shapiro

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: To compare lymphocyte immunohistochemical markers and staining for Epstein-Barr virus (EBV) in tonsillectomy specimens from healthy children and pediatric transplant recipients. Design: Analysis of pathology specimens. Setting: Tertiary care medical center. Patients: Consecutive sample of tonsillectomy specimens from 60 pediatric solid organ transplant recipients and 60 healthy children. Intervention: Immunohistochemical staining of tonsillectomy specimens for κ and λ light chains, B and T lymphocytes, EBV-encoded small nuclear RNA (EBV-EBER), and EBV-encoded latent membrane protein (EBV-LMP). Main Outcome Measure: Detection of a difference in EBV activity in transplant recipients vs healthy controls. Results: There was 1 case of posttransplantation lymphoproliferative disorder (PTLD). All other tonsillectomy specimens from both groups demonstrated follicular hyperplasia. Tonsillectomy specimens from both groups were polyclonal, expressing κ and λ light-chain activity, including the case of PTLD. The number of specimens staining positive for CD3 activity, a marker of T lymphocytes, was reduced in the transplant group (85%), compared with 100% in the control group (P<.01). EBV-EBER is a nuclear stain indicating active EBV infection, whereas EBV-LMP staining denotes latent infection. Twenty-seven of 60 transplant specimens (45%) demonstrated EBV-EBER activity compared with 0 of 60 control specimens (P<.001). EBV-LMP activity was equal in both groups. Conclusions: Adenotonsillar hypertrophy in transplant recipients with no prior exposure to EBV may be a sign of active EBV infection. A high incidence of EBV-EBER was found in the tonsils of transplant recipients. Active adenotonsillar EBV infection in the setting of T-lymphocyte suppression in transplant recipients may be a potential early precursor of PTLD.

Original languageEnglish (US)
Pages (from-to)936-939
Number of pages4
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume134
Issue number9
DOIs
StatePublished - Sep 1 2008

Fingerprint

Palatine Tonsil
Human Herpesvirus 4
Tonsillectomy
Epstein-Barr Virus Infections
Lymphoproliferative Disorders
Staining and Labeling
T-Lymphocytes
Transplants
Transplant Recipients
Pediatrics
Small Nuclear RNA
Light
Tertiary Care Centers
Hypertrophy
Hyperplasia
Membrane Proteins
B-Lymphocytes
Coloring Agents
Outcome Assessment (Health Care)
Lymphocytes

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Immunohistochemical analysis and Epstein-Barr virus in the tonsils of transplant recipients and healthy controls. / Mowry, Sarah E.; Strocker, Ali M.; Chan, Jessica; Takehana, Christopher; Kalantar, Nader; Bhuta, Sunita; Shapiro, Nina L.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 134, No. 9, 01.09.2008, p. 936-939.

Research output: Contribution to journalArticle

Mowry, Sarah E. ; Strocker, Ali M. ; Chan, Jessica ; Takehana, Christopher ; Kalantar, Nader ; Bhuta, Sunita ; Shapiro, Nina L. / Immunohistochemical analysis and Epstein-Barr virus in the tonsils of transplant recipients and healthy controls. In: Archives of Otolaryngology - Head and Neck Surgery. 2008 ; Vol. 134, No. 9. pp. 936-939.
@article{17b6e9ae37df4ceb81322d9449e7c3c7,
title = "Immunohistochemical analysis and Epstein-Barr virus in the tonsils of transplant recipients and healthy controls",
abstract = "Objective: To compare lymphocyte immunohistochemical markers and staining for Epstein-Barr virus (EBV) in tonsillectomy specimens from healthy children and pediatric transplant recipients. Design: Analysis of pathology specimens. Setting: Tertiary care medical center. Patients: Consecutive sample of tonsillectomy specimens from 60 pediatric solid organ transplant recipients and 60 healthy children. Intervention: Immunohistochemical staining of tonsillectomy specimens for κ and λ light chains, B and T lymphocytes, EBV-encoded small nuclear RNA (EBV-EBER), and EBV-encoded latent membrane protein (EBV-LMP). Main Outcome Measure: Detection of a difference in EBV activity in transplant recipients vs healthy controls. Results: There was 1 case of posttransplantation lymphoproliferative disorder (PTLD). All other tonsillectomy specimens from both groups demonstrated follicular hyperplasia. Tonsillectomy specimens from both groups were polyclonal, expressing κ and λ light-chain activity, including the case of PTLD. The number of specimens staining positive for CD3 activity, a marker of T lymphocytes, was reduced in the transplant group (85{\%}), compared with 100{\%} in the control group (P<.01). EBV-EBER is a nuclear stain indicating active EBV infection, whereas EBV-LMP staining denotes latent infection. Twenty-seven of 60 transplant specimens (45{\%}) demonstrated EBV-EBER activity compared with 0 of 60 control specimens (P<.001). EBV-LMP activity was equal in both groups. Conclusions: Adenotonsillar hypertrophy in transplant recipients with no prior exposure to EBV may be a sign of active EBV infection. A high incidence of EBV-EBER was found in the tonsils of transplant recipients. Active adenotonsillar EBV infection in the setting of T-lymphocyte suppression in transplant recipients may be a potential early precursor of PTLD.",
author = "Mowry, {Sarah E.} and Strocker, {Ali M.} and Jessica Chan and Christopher Takehana and Nader Kalantar and Sunita Bhuta and Shapiro, {Nina L.}",
year = "2008",
month = "9",
day = "1",
doi = "10.1001/archotol.134.9.936",
language = "English (US)",
volume = "134",
pages = "936--939",
journal = "JAMA Otolaryngology - Head and Neck Surgery",
issn = "2168-6181",
publisher = "American Medical Association",
number = "9",

}

TY - JOUR

T1 - Immunohistochemical analysis and Epstein-Barr virus in the tonsils of transplant recipients and healthy controls

AU - Mowry, Sarah E.

AU - Strocker, Ali M.

AU - Chan, Jessica

AU - Takehana, Christopher

AU - Kalantar, Nader

AU - Bhuta, Sunita

AU - Shapiro, Nina L.

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Objective: To compare lymphocyte immunohistochemical markers and staining for Epstein-Barr virus (EBV) in tonsillectomy specimens from healthy children and pediatric transplant recipients. Design: Analysis of pathology specimens. Setting: Tertiary care medical center. Patients: Consecutive sample of tonsillectomy specimens from 60 pediatric solid organ transplant recipients and 60 healthy children. Intervention: Immunohistochemical staining of tonsillectomy specimens for κ and λ light chains, B and T lymphocytes, EBV-encoded small nuclear RNA (EBV-EBER), and EBV-encoded latent membrane protein (EBV-LMP). Main Outcome Measure: Detection of a difference in EBV activity in transplant recipients vs healthy controls. Results: There was 1 case of posttransplantation lymphoproliferative disorder (PTLD). All other tonsillectomy specimens from both groups demonstrated follicular hyperplasia. Tonsillectomy specimens from both groups were polyclonal, expressing κ and λ light-chain activity, including the case of PTLD. The number of specimens staining positive for CD3 activity, a marker of T lymphocytes, was reduced in the transplant group (85%), compared with 100% in the control group (P<.01). EBV-EBER is a nuclear stain indicating active EBV infection, whereas EBV-LMP staining denotes latent infection. Twenty-seven of 60 transplant specimens (45%) demonstrated EBV-EBER activity compared with 0 of 60 control specimens (P<.001). EBV-LMP activity was equal in both groups. Conclusions: Adenotonsillar hypertrophy in transplant recipients with no prior exposure to EBV may be a sign of active EBV infection. A high incidence of EBV-EBER was found in the tonsils of transplant recipients. Active adenotonsillar EBV infection in the setting of T-lymphocyte suppression in transplant recipients may be a potential early precursor of PTLD.

AB - Objective: To compare lymphocyte immunohistochemical markers and staining for Epstein-Barr virus (EBV) in tonsillectomy specimens from healthy children and pediatric transplant recipients. Design: Analysis of pathology specimens. Setting: Tertiary care medical center. Patients: Consecutive sample of tonsillectomy specimens from 60 pediatric solid organ transplant recipients and 60 healthy children. Intervention: Immunohistochemical staining of tonsillectomy specimens for κ and λ light chains, B and T lymphocytes, EBV-encoded small nuclear RNA (EBV-EBER), and EBV-encoded latent membrane protein (EBV-LMP). Main Outcome Measure: Detection of a difference in EBV activity in transplant recipients vs healthy controls. Results: There was 1 case of posttransplantation lymphoproliferative disorder (PTLD). All other tonsillectomy specimens from both groups demonstrated follicular hyperplasia. Tonsillectomy specimens from both groups were polyclonal, expressing κ and λ light-chain activity, including the case of PTLD. The number of specimens staining positive for CD3 activity, a marker of T lymphocytes, was reduced in the transplant group (85%), compared with 100% in the control group (P<.01). EBV-EBER is a nuclear stain indicating active EBV infection, whereas EBV-LMP staining denotes latent infection. Twenty-seven of 60 transplant specimens (45%) demonstrated EBV-EBER activity compared with 0 of 60 control specimens (P<.001). EBV-LMP activity was equal in both groups. Conclusions: Adenotonsillar hypertrophy in transplant recipients with no prior exposure to EBV may be a sign of active EBV infection. A high incidence of EBV-EBER was found in the tonsils of transplant recipients. Active adenotonsillar EBV infection in the setting of T-lymphocyte suppression in transplant recipients may be a potential early precursor of PTLD.

UR - http://www.scopus.com/inward/record.url?scp=52249089450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52249089450&partnerID=8YFLogxK

U2 - 10.1001/archotol.134.9.936

DO - 10.1001/archotol.134.9.936

M3 - Article

C2 - 18794437

AN - SCOPUS:52249089450

VL - 134

SP - 936

EP - 939

JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

SN - 2168-6181

IS - 9

ER -